scholarly journals Biocompatible Polymer–Peptide Hybrid-Based DNA Nanoparticles for Gene Delivery

2015 ◽  
Vol 7 (19) ◽  
pp. 10446-10456 ◽  
Author(s):  
Dominik Witzigmann ◽  
Dalin Wu ◽  
Susanne H. Schenk ◽  
Vimalkumar Balasubramanian ◽  
Wolfgang Meier ◽  
...  
Keyword(s):  
Gene Delivery ◽  
Biocompatible Polymer ◽  
Dna Nanoparticles

Cationic amphiphilic calixarenes to compact DNA into small nanoparticles for gene delivery

2015 ◽  
Vol 39 (3) ◽  
pp. 1654-1664 ◽  
Author(s):  
Roman V. Rodik ◽  
Anne-Sophie Anthony ◽  
Vitaly I. Kalchenko ◽  
Yves Mély ◽  
Andrey S. Klymchenko
Keyword(s):  
Gene Delivery ◽  
Transfection Efficiency ◽  
Alkyl Chains ◽  
Dna Nanoparticles ◽  
Small Nanoparticles

Cationic amphiphilic calixarenes with longer alkyl chains are less cytotoxic and their virus-sized DNA nanoparticles exhibit higher transfection efficiency.

Surface Coating Approach to Overcome Mucosal Entrapment of DNA Nanoparticles for Oral Gene Delivery of Glucagon-like Peptide 1

2019 ◽  
Vol 11 (33) ◽  
pp. 29593-29603 ◽  
Author(s):  
Tianqi Nie ◽  
Zhiyu He ◽  
Yang Zhou ◽  
Jinchang Zhu ◽  
Kuntao Chen ◽  
...  
Keyword(s):  
Gene Delivery ◽  
Surface Coating ◽  
Dna Nanoparticles ◽  
Glucagon Like Peptide ◽  
Glucagon Like Peptide 1

Preparation and Analysis of PEGylated Poly-L-Lysine DNA Nanoparticles for Gene Delivery

2010 ◽  
Vol 2010 (5) ◽  
pp. pdb.prot5419-pdb.prot5419
Author(s):  
P. B. Davis ◽  
T. H. Kowalczyk
Keyword(s):  
Gene Delivery ◽  
Dna Nanoparticles

Dual-functionalized calcium carbonate based gene delivery system for efficient gene delivery

RSC Advances ◽  
2014 ◽  
Vol 4 (73) ◽  
pp. 38623-38629 ◽  
Author(s):  
Chao-Qun Wang ◽  
Meng-Qing Gong ◽  
Jin-Long Wu ◽  
Ren-Xi Zhuo ◽  
Si-Xue Cheng
Keyword(s):  
Calcium Carbonate ◽  
Gene Delivery ◽  
Delivery System ◽  
Gene Transfection ◽  
Cell Penetrating Peptide ◽  
Gene Delivery System ◽  
Dna Nanoparticles ◽  
Efficient Gene ◽  
Cell Penetrating ◽  
A Cell

Dual-functionalized KALA/PS/CaCO3/DNA nanoparticles containing a cell penetrating peptide (KALA) and protamine sulfate (PS) could effectively mediate gene transfection at a low DNA concentration.

scholarly journals Targeted cationic poly(D,L-lactic-co-glycolic acid) nanoparticles for gene delivery to cultured cells

2009 ◽  
Vol 14 (2) ◽  
Author(s):  
Sonsoles Díez ◽  
Itziar Miguéliz ◽  
Conchita Tros de Ilarduya
Keyword(s):  
Gene Delivery ◽  
Glycolic Acid ◽  
Cultured Cells ◽  
Interleukin 12 ◽  
High Concentration ◽  
Dna Nanoparticles ◽  
Potential System ◽  
Genes Encoding ◽  
Solvent Evaporation Process

AbstractWe developed a new targeted cationic nanoparticulate system composed of poly(D,L-lactic-co-glycolic acid) (PLGA), 1,2-dioleoyl-3-(trimethylammonium) propane (DOTAP) and asialofetuin (AF), and found it to be a highly effective formulation for gene delivery to liver tumor cells. The nanoparticles (NP) were prepared by a modified solvent evaporation process that used two protocols in order to encapsulate (NP1 particles) or adsorb (NP2 particles) plasmid DNA. The final particles are in the nanoscale range. pDNA loaded in PLGA/DOTAP/AF particles with high loading efficiency showed a positive surface charge. Targeted asialofetuin-nanoparticles (AF-NP) carrying genes encoding for luciferase and interleukin-12 (IL-12) resulted in increased transfection efficiencies compared to free DNA and to plain (non-targeted) systems, even in the presence of 60% fetal bovine serum (FBS). The results of transfections performed on HeLa cells, defective in asialoglycoprotein receptors (ASGPr-), confirmed the receptor-mediated endocytosis mechanism. In summary, this is the first time that asialoglycoprotein receptor targeting by PLGA/DOTAP/DNA nanoparticles carrying the therapeutic gene IL-12 has been shown to be efficient in gene delivery to liver cancer cells in the presence of a very high concentration of serum, and this could be a potential system for in vivo application.

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