Ultrafine Pd Nanoparticles Encapsulated in Microporous Co3O4Hollow Nanospheres for In Situ Molecular Detection of Living Cells

2015 ◽  
Vol 7 (9) ◽  
pp. 5583-5590 ◽  
Author(s):  
Jiangbo Xi ◽  
Yan Zhang ◽  
Ning Wang ◽  
Lin Wang ◽  
Zheye Zhang ◽  
...  
Keyword(s):  
Molecular Detection ◽  
Pd Nanoparticles ◽  
Living Cells

scholarly journals A sensitive and specific method for microRNA detection and in situ imaging based on a CRISPR–Cas9 modified catalytic hairpin assembly

RSC Advances ◽  
2020 ◽  
Vol 10 (47) ◽  
pp. 28037-28040 ◽  
Author(s):  
Yang Liu ◽  
Shihong Li ◽  
Likun Zhang ◽  
Qian Zhao ◽  
Nuo Li ◽  
...  
Keyword(s):  
Molecular Detection ◽  
Living Cells ◽  
Specific Method ◽  
Microrna Detection ◽  
Catalytic Hairpin Assembly ◽  
In Situ Imaging

We report here a method for the molecular detection of miRNAs in exosomes and imaging in living cells based on CRISPR–Cas9 and catalytic hairpin assembly.

DNA nanolantern-based split aptamer probes for in-situ ATP imaging in living cells and lighting up mitochondria

The Analyst ◽  
2021 ◽  
Author(s):  
Yaxin Wang ◽  
Dong-Xia Wang ◽  
Jia-Yi Ma ◽  
Jing Wang ◽  
Yichen Du ◽  
...  
Keyword(s):  
Adenosine Triphosphate ◽  
Cell Metabolism ◽  
Living Cells ◽  
Expression Level ◽  
Specific Analysis

Accurate and specific analysis of adenosine triphosphate (ATP) expression level in living cells can provide valuable information for understanding cell metabolism, physiological activities and pathologic mechanism. Herein, DNA nanolantern-based split...

In situ mercaptosilane-assisted confinement of Pd nanoparticles in Beta for high-efficient methane oxidation

2021 ◽  
Author(s):  
Liling Zhang ◽  
Junfei Chen ◽  
Haolin Yang ◽  
Xiaohan Wang ◽  
Zebao Rui
Keyword(s):  
Methane Oxidation ◽  
Pd Nanoparticles ◽  
High Efficient

scholarly journals Aptamer Nanoflares for Molecular Detection in Living Cells*

2020 ◽  
pp. 1727-1737
Author(s):  
Dan Zheng ◽  
Dwight S. Seferos ◽  
David A. Giljohann ◽  
Pinal C. Patel ◽  
Chad A. Mirkin
Keyword(s):  
Molecular Detection ◽  
Living Cells

scholarly journals Transport and localization of exogenous myelin basic protein mRNA microinjected into oligodendrocytes.

1993 ◽  
Vol 123 (2) ◽  
pp. 431-441 ◽  
Author(s):  
K Ainger ◽  
D Avossa ◽  
F Morgan ◽  
S J Hill ◽  
C Barry ◽  
...  
Keyword(s):  
Three Dimensional ◽  
Living Cells ◽  
Branch Points ◽  
Rna Granules ◽  
Intracellular Movement ◽  
Directional Movement ◽  
Ionic Detergent ◽  
Mrna Granules

We have studied transport and localization of MBP mRNA in oligodendrocytes in culture by microinjecting labeled mRNA into living cells and analyzing the intracellular distribution of the injected RNA by confocal microscopy. Injected mRNA initially appears dispersed in the perikaryon. Within minutes, the RNA forms granules which, in the case of MBP mRNA, are transported down the processes to the periphery of the cell where the distribution again becomes dispersed. In situ hybridization shows that endogenous MBP mRNA in oligodendrocytes also appears as granules in the perikaryon and processes and dispersed in the peripheral membranes. The granules are not released by extraction with non-ionic detergent, indicating that they are associated with the cytoskeletal matrix. Three dimensional visualization indicates that MBP mRNA granules are often aligned in tracks along microtubules traversing the cytoplasm and processes. Several distinct patterns of granule movement are observed. Granules in the processes undergo sustained directional movement with a velocity of approximately 0.2 micron/s. Granules at branch points undergo oscillatory motion with a mean displacement of 0.1 micron/s. Granules in the periphery of the cell circulate randomly with a mean displacement of approximately 1 micron/s. The results are discussed in terms of a multi-step pathway for transport and localization of MBP mRNA in oligodendrocytes. This work represents the first characterization of intracellular movement of mRNA in living cells, and the first description of the role of RNA granules in transport and localization of mRNA in cells.

In Situ Amplification‐Based Imaging of RNA in Living Cells

2019 ◽  
Vol 131 (34) ◽  
pp. 11698-11709 ◽  
Author(s):  
Zhihe Qing ◽  
Jingyuan Xu ◽  
Jinlei Hu ◽  
Jing Zheng ◽  
Lei He ◽  
...  
Keyword(s):  
Living Cells
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