Enzyme-Induced Transformable Peptide Nanocarriers with Enhanced Drug Permeability and Retention to Improve Tumor Nanotherapy Efficacy

2021 ◽  
Author(s):  
Zhongying Gong ◽  
Baolong Zhou ◽  
Xiaoying Liu ◽  
Juanjuan Cao ◽  
Zexin Hong ◽  
...  
Keyword(s):  
Drug Permeability

Drug permeability and mucoadhesion properties of thiolated trimethyl chitosan nanoparticles in oral insulin delivery

Biomaterials ◽  
2009 ◽  
Vol 30 (29) ◽  
pp. 5691-5700 ◽  
Author(s):  
Lichen Yin ◽  
Jieying Ding ◽  
Chunbai He ◽  
Liming Cui ◽  
Cui Tang ◽  
...  
Keyword(s):  
Chitosan Nanoparticles ◽  
Insulin Delivery ◽  
Oral Insulin ◽  
Drug Permeability ◽  
Trimethyl Chitosan

scholarly journals Human Induced Pluripotent Stem Cell-Based Skin for Assessing Transdermal Drug Permeability and Irritancy

2021 ◽  
Vol 44 (1) ◽  
pp. 140-143
Author(s):  
Chihiro Naito ◽  
Tomoko Yamaguchi ◽  
Hidemasa Katsumi ◽  
Suyo Kimura ◽  
Sachi Kamei ◽  
...  
Keyword(s):  
Stem Cell ◽  
Pluripotent Stem Cell ◽  
Induced Pluripotent Stem Cell ◽  
Drug Permeability ◽  
Induced Pluripotent

scholarly journals Ultrasound Microbubble Treatment Enhances Clathrin-Mediated Endocytosis and Fluid-Phase Uptake through Distinct Mechanisms

2021 ◽  
Author(s):  
Farnaz Fekri ◽  
Ralph Christian Delos Santos ◽  
Raffi Karshafian ◽  
Costin N. Antonescu
Keyword(s):  
Drug Delivery ◽  
Fluid Phase ◽  
Acid Sphingomyelinase ◽  
Coated Pits ◽  
Drug Permeability ◽  
Membrane Pores ◽  
Signaling Mechanisms ◽  
Sheer Stress ◽  
Cell Plasma ◽  
Fluid Phase Endocytosis

Drug delivery to tumors is limited by several factors, including drug permeability of the target cell plasma membrane. Ultrasound in combination with microbubbles (USMB) is a promising strategy to overcome these limitations. USMB treatment elicits enhanced cellular uptake of materials such as drugs, in part as a result of sheer stress and formation of transient membrane pores. Pores formed upon USMB treatment are rapidly resealed, suggesting that other processes such as enhanced endocytosis may contribute to the enhanced material uptake by cells upon USMB treatment. How USMB regulates endocytic processes remains incompletely understood. Cells constitutively utilize several distinct mechanisms of endocytosis, including clathrin-mediated endocytosis (CME) for the internalization of receptor-bound macromolecules such as Transferrin Receptor (TfR), and distinct mechanism(s) that mediate the majority of fluid-phase endocytosis. Tracking the abundance of TfR on the cell surface and the internalization of its ligand transferrin revealed that USMB acutely enhances the rate of CME. Total internal reflection fluorescence microscopy experiments revealed that USMB treatment altered the assembly of clathrin-coated pits, the basic structural units of CME. In addition, the rate of fluid-phase endocytosis was enhanced, but with delayed onset upon USMB treatment relative to the enhancement of CME, suggesting that the two processes are distinctly regulated by USMB. Indeed, vacuolin-1 or desipramine treatment prevented the enhancement of CME but not of fluid phase endocytosis upon USMB, suggesting that lysosome exocytosis and acid sphingomyelinase, respectively, are required for the regulation of CME but not fluid phase endocytosis upon USMB treatment. These results indicate that USMB enhances both CME and fluid phase endocytosis through distinct signaling mechanisms, and suggest that strategies for potentiating the enhancement of endocytosis upon USMB treatment may improve targeted drug delivery.

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