One-Step Approach to Prepare Transparent Conductive Regenerated Silk Fibroin/PEDOT:PSS Films for Electroactive Cell Culture

2021 ◽  
Author(s):  
Ao Zhuang ◽  
Xiangyu Huang ◽  
Suna Fan ◽  
Xiang Yao ◽  
Bo Zhu ◽  
...  
Keyword(s):  
Cell Culture ◽  
Silk Fibroin ◽  
Regenerated Silk Fibroin ◽  
One Step

scholarly journals Is Dialdehyde Chitosan a Good Substance to Modify Physicochemical Properties of Biopolymeric Materials?

2021 ◽  
Vol 22 (7) ◽  
pp. 3391
Author(s):  
Sylwia Grabska-Zielińska ◽  
Alina Sionkowska ◽  
Ewa Olewnik-Kruszkowska ◽  
Katarzyna Reczyńska ◽  
Elżbieta Pamuła
Keyword(s):  
Mechanical Properties ◽  
Tissue Engineering ◽  
Physicochemical Properties ◽  
Silk Fibroin ◽  
Three Dimensional ◽  
Microscopy Imaging ◽  
Maximum Deformation ◽  
One Step ◽  
Chitosan Blends ◽  
Fourier Transfer Infrared Spectroscopy

The aim of this work was to compare physicochemical properties of three dimensional scaffolds based on silk fibroin, collagen and chitosan blends, cross-linked with dialdehyde starch (DAS) and dialdehyde chitosan (DAC). DAS was commercially available, while DAC was obtained by one-step synthesis. Structure and physicochemical properties of the materials were characterized using Fourier transfer infrared spectroscopy with attenuated total reflectance device (FTIR-ATR), swelling behavior and water content measurements, porosity and density observations, scanning electron microscopy imaging (SEM), mechanical properties evaluation and thermogravimetric analysis. Metabolic activity with AlamarBlue assay and live/dead fluorescence staining were performed to evaluate the cytocompatibility of the obtained materials with MG-63 osteoblast-like cells. The results showed that the properties of the scaffolds based on silk fibroin, collagen and chitosan can be modified by chemical cross-linking with DAS and DAC. It was found that DAS and DAC have different influence on the properties of biopolymeric scaffolds. Materials cross-linked with DAS were characterized by higher swelling ability (~4000% for DAS cross-linked materials; ~2500% for DAC cross-linked materials), they had lower density (Coll/CTS/30SF scaffold cross-linked with DAS: 21.8 ± 2.4 g/cm3; cross-linked with DAC: 14.6 ± 0.7 g/cm3) and lower mechanical properties (maximum deformation for DAC cross-linked scaffolds was about 69%; for DAS cross-linked scaffolds it was in the range of 12.67 ± 1.51% and 19.83 ± 1.30%) in comparison to materials cross-linked with DAC. Additionally, scaffolds cross-linked with DAS exhibited higher biocompatibility than those cross-linked with DAC. However, the obtained results showed that both types of scaffolds can provide the support required in regenerative medicine and tissue engineering. The scaffolds presented in the present work can be potentially used in bone tissue engineering to facilitate healing of small bone defects.

Silk-based hybrid microfibrous mats as guided bone regeneration membranes

2021 ◽  
Author(s):  
Mi Wu ◽  
Zhengyi Han ◽  
Wen Liu ◽  
Jinrong Yao ◽  
Bingjiao Zhao ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Cell Adhesion ◽  
Bone Regeneration ◽  
Silk Fibroin ◽  
Guided Bone Regeneration ◽  
Regenerated Silk Fibroin ◽  
Marrow Mesenchymal Stem Cells ◽  
Cell Adhesion And Proliferation

LAPONITE® (LAP) nanoplatelets were incorporated within a regenerated silk fibroin (RSF) microfibrous mat via electrospinning, which exhibited better cell adhesion and proliferation of bone marrow mesenchymal stem cells (BMSCs) than the pristine RSF ones.

Some comments on the penetration of water vapor into regenerated silk fibroin

Polymer ◽  
2001 ◽  
Vol 42 (12) ◽  
pp. 5495-5499 ◽  
Author(s):  
Y. Hirai ◽  
J. Ishikuro ◽  
T. Nakajima
Keyword(s):  
Water Vapor ◽  
Silk Fibroin ◽  
Regenerated Silk Fibroin

Rheology and microstructural studies of regenerated silk fibroin solutions

2013 ◽  
Vol 52 (10-12) ◽  
pp. 833-840 ◽  
Author(s):  
Anuya Nisal ◽  
Chirag Kalelkar ◽  
Jayesh Bellare ◽  
Ashish Lele
Keyword(s):  
Silk Fibroin ◽  
Regenerated Silk Fibroin ◽  
Microstructural Studies

SILK FIBROIN FILMS CRYSTALLIZED BY MULTIWALLED CARBON NANOTUBES

2008 ◽  
Vol 22 (09n11) ◽  
pp. 1807-1812 ◽  
Author(s):  
H.-S. KIM ◽  
W.-I. PARK ◽  
Y. KIM ◽  
H.-J. JIN
Keyword(s):  
Electron Microscopy ◽  
Carbon Nanotubes ◽  
Multiwalled Carbon Nanotubes ◽  
Silk Fibroin ◽  
Composite Films ◽  
Tensile Modulus ◽  
Multiwalled Carbon ◽  
Regenerated Silk Fibroin ◽  
Silk Films ◽  
Β Sheet

Silk films prepared from regenerated silk fibroin are normally stabilized by β-sheet formation through the use of solvents (methanol, water etc.). Herein, we report a new method of preparing water-stable films without a β-sheet conformation from regenerated silk fibroin solutions by incorporating a small amount (0.2 wt%) of multiwalled carbon nanotubes (MWCNTs). To extend the biomaterial utility of silk proteins, forming water-stable silk-based materials with enhanced mechanical properties is essential. Scanning electron microscopy and transmission electron microscopy were used to observe the morphology of the MWCNT-incorporated silk films. The wide-angle X-ray diffraction provided clear evidence of the crystallization of the silk fibroin induced by MWCNT in the composite films without any additional annealing processing. The tensile modulus and strength of the composite films were improved by 108% and 51%, respectively, by the incorporation of 0.2 wt% of MWCNTs, as compared with those of the pure silk films. The method described in this study will provide an alternative means of crystallizing silk fibroin films without using an organic solvent or blending with any other polymers, which may be important in biomedical applications.

Regenerated silk fibroin-based dressing modified with carnosine-bentonite nanosheets accelerates healing of second-degree burn wound

2020 ◽  
Vol 74 (10) ◽  
pp. 3243-3257
Author(s):  
Pegah Azimzadeh Asiabi ◽  
Ali Ramazani ◽  
Mehdi Khoobi ◽  
Mohsen Amin ◽  
Marzieh Shakoori ◽  
...  
Keyword(s):  
Silk Fibroin ◽  
Burn Wound ◽  
Degree Burn ◽  
Regenerated Silk Fibroin ◽  
Second Degree

scholarly journals Reactive Inkjet Printing of Regenerated Silk Fibroin Films for Use as Dental Barrier Membranes

Micromachines ◽  
2018 ◽  
Vol 9 (2) ◽  
pp. 46 ◽  
Author(s):  
Patrick Rider ◽  
Ian. Brook ◽  
Patrick Smith ◽  
Cheryl Miller
Keyword(s):  
Silk Fibroin ◽  
Inkjet Printing ◽  
Barrier Membranes ◽  
Regenerated Silk Fibroin
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