Design of Swollen Lipidic Cubic Phase to Increase Transcutaneous Penetration of Biomacromolecules

2021 ◽  
Author(s):  
Shuto Kozaka ◽  
Rie Wakabayashi ◽  
Noriho Kamiya ◽  
Masahiro Goto
Keyword(s):  
Cubic Phase ◽  
Lipidic Cubic Phase

scholarly journals Exploring the structural relationship between encapsulated antimicrobial peptides and the bilayer membrane mimetic lipidic cubic phase: studies with gramicidin A′

RSC Advances ◽  
2016 ◽  
Vol 6 (73) ◽  
pp. 68685-68694 ◽  
Author(s):  
Thomas G. Meikle ◽  
Charlotte E. Conn ◽  
Frances Separovic ◽  
Calum J. Drummond
Keyword(s):  
Antimicrobial Peptides ◽  
Low Cost ◽  
Bilayer Membrane ◽  
Structural Relationship ◽  
Gramicidin A ◽  
Cubic Phase ◽  
Membrane Peptides ◽  
Membrane Mimetic ◽  
Lipidic Cubic Phase ◽  
Bicontinuous Cubic

Lipid based bicontinuous cubic mesophases provide a low-cost, robust membrane mimetic nanomaterial which allows for the incorporation of membrane peptides and proteins.

scholarly journals The Lipidic Cubic Phase as a Membrane Mimetic

2011 ◽  
Vol 100 (8) ◽  
pp. 2075 ◽  
Author(s):  
Nicole Höfer ◽  
David Aragão ◽  
Martin Caffrey
Keyword(s):  
Cubic Phase ◽  
Membrane Mimetic ◽  
Lipidic Cubic Phase

Stability, flow alignment and a phase transition of the lipidic cubic phase during continuous flow injection

2021 ◽  
Author(s):  
Peter Berntsen ◽  
Connie Darmanin ◽  
Eugeniu Balaur ◽  
Leonie Flueckiger ◽  
Alex Kozlov ◽  
...  
Keyword(s):  
Phase Transition ◽  
Flow Injection ◽  
Continuous Flow ◽  
Cubic Phase ◽  
Lipidic Cubic Phase ◽  
Flow Alignment

SANS investigations of the lipidic cubic phase behaviour in course of bacteriorhodopsin crystallization

2005 ◽  
Vol 275 (1-2) ◽  
pp. e1453-e1459 ◽  
Author(s):  
R. Efremov ◽  
G. Shiryaeva ◽  
G. Bueldt ◽  
A. Islamov ◽  
A. Kuklin ◽  
...  
Keyword(s):  
Phase Behaviour ◽  
Cubic Phase ◽  
Lipidic Cubic Phase

scholarly journals Acoustic Methods to Monitor Protein Crystallization and to Detect Protein Crystals in Suspensions of Agarose and Lipidic Cubic Phase

2016 ◽  
Vol 21 (1) ◽  
pp. 107-114 ◽  
Author(s):  
Daniel L. Ericson ◽  
Xingyu Yin ◽  
Alexander Scalia ◽  
Yasmin N. Samara ◽  
Richard Stearns ◽  
...  
Keyword(s):  
Protein Crystallization ◽  
Protein Crystals ◽  
Cubic Phase ◽  
Acoustic Methods ◽  
Lipidic Cubic Phase

scholarly journals Microscope detection options for colorless protein crystals grown in lipidic cubic phases

2003 ◽  
Vol 36 (5) ◽  
pp. 1295-1296 ◽  
Author(s):  
Peter Nollert
Keyword(s):  
Membrane Proteins ◽  
Crystal Nucleation ◽  
Protein Crystal ◽  
Protein Crystals ◽  
Cubic Phase ◽  
Polarization Microscopy ◽  
Cubic Phases ◽  
Lipidic Cubic Phase ◽  
Surface Distortions ◽  
Microscopic Techniques

The use of lipidic cubic phases as crystal nucleation and growth matrices is becoming popular and has yielded crystals of soluble and membrane proteins. So far, all of the membrane proteins crystallized by this method have been colored. This feature has facilitated the detection of the often encountered microcrystals in initial screening rounds. Indeed, small colorless protein crystals have poor optical contrast as a result of the small differences in refractive index of the protein crystal and the surrounding lipidic cubic phase. While a perfect preparation of a lipidic cubic phase is transparent and optically isotropic, in a crystallization setup it frequently disguises crystals due to cracks, inclusions, surface distortions and phase boundaries. Here, several specialized microscopic techniques and illumination conditions are compared and it is found that sufficient contrast is generated by cross polarization microscopy and by Hoffman modulation contrast microscopy for the detection of colorless protein crystals.

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