Dual-Modality Poly-l-histidine Nanoparticles to Deliver Peptide Nucleic Acids and Paclitaxel for In Vivo Cancer Therapy

2021 ◽  
Author(s):  
Aniket Wahane ◽  
Shipra Malik ◽  
Kuo-Chih Shih ◽  
Ravinder Reddy Gaddam ◽  
Chaohao Chen ◽  
...  
Keyword(s):  
Cancer Therapy ◽  
Nucleic Acids ◽  
Peptide Nucleic Acids ◽  
Dual Modality

Peptide nucleic acids specifically cause antigene effects in vivo by systemic injection

Life Sciences ◽  
2002 ◽  
Vol 71 (3) ◽  
pp. 325-337 ◽  
Author(s):  
Beth M. McMahon ◽  
Jennifer A. Stewart ◽  
M.D. Bitner ◽  
Abdul Fauq ◽  
Daniel J. McCormick ◽  
...  
Keyword(s):  
Nucleic Acids ◽  
Peptide Nucleic Acids ◽  
Systemic Injection

Hydroxyproline-Based DNA Mimics: A Review on Synthesis and Properties

2006 ◽  
Vol 71 (7) ◽  
pp. 929-955 ◽  
Author(s):  
Vladimir A. Efimov ◽  
Oksana G. Chakhmakhcheva
Keyword(s):  
Nucleic Acid ◽  
Nucleic Acids ◽  
Phosphonic Acid ◽  
Peptide Nucleic Acids ◽  
Biological Properties ◽  
High Potential ◽  
Physicochemical And Biological Properties

With the aim to improve physicochemical and biological properties of natural oligonucleotides, many types of DNA analogues and mimics are designed on the basis of hydroxyproline and its derivatives, and their properties are evaluated. Among them, two types of DNA mimics representing hetero-oligomers constructed from alternating monomers of phosphono peptide nucleic acids and monomers on the base of trans-1-acetyl-4-hydroxy-L-proline (HypNA-pPNAs) and oligomers constructed from monomers containing (2S,4R)-1-acetyl-4-hydroxypyrrolidine-2-phosphonic acid backbone (pHypNAs) are of particular interest. In a set of in vitro and in vivo assays, it was shown that HypNA-pPNAs and pHypNAs demonstrated a high potential for the use in nucleic acid based diagnostics, isolation of nucleic acids and antisense experiments. A review with 53 references.

Specific gene blockade shows that peptide nucleic acids readily enter neuronal cells in vivo

FEBS Letters ◽  
1998 ◽  
Vol 421 (3) ◽  
pp. 280-284 ◽  
Author(s):  
Beth M Tyler ◽  
Daniel J McCormick ◽  
Clark V Hoshall ◽  
Christopher L Douglas ◽  
Karen Jansen ◽  
...  
Keyword(s):  
Nucleic Acids ◽  
Peptide Nucleic Acids ◽  
Neuronal Cells ◽  
Specific Gene

Synthetic α-l-Threose Nucleic Acids Targeting BcL-2 Show Gene Silencing and in Vivo Antitumor Activity for Cancer Therapy

2019 ◽  
Vol 11 (42) ◽  
pp. 38510-38518 ◽  
Author(s):  
Fei Wang ◽  
Ling Sum Liu ◽  
Cia Hin Lau ◽  
Tristan Juin Han Chang ◽  
Dick Yan Tam ◽  
...  
Keyword(s):  
Cancer Therapy ◽  
Antitumor Activity ◽  
Nucleic Acids ◽  
Gene Silencing ◽  
In Vivo Antitumor Activity

scholarly journals Exploring miR-9 Involvement in Ciona intestinalis Neural Development Using Peptide Nucleic Acids

2020 ◽  
Vol 21 (6) ◽  
pp. 2001
Author(s):  
Silvia Mercurio ◽  
Silvia Cauteruccio ◽  
Raoul Manenti ◽  
Simona Candiani ◽  
Giorgio Scarì ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Nucleic Acids ◽  
Neural Development ◽  
Peptide Nucleic Acids ◽  
Model Organism ◽  
Ciona Intestinalis ◽  
Transcriptional Level ◽  
Regulate Gene Expression

The microRNAs are small RNAs that regulate gene expression at the post-transcriptional level and can be involved in the onset of neurodegenerative diseases and cancer. They are emerging as possible targets for antisense-based therapy, even though the in vivo stability of miRNA analogues is still questioned. We tested the ability of peptide nucleic acids, a novel class of nucleic acid mimics, to downregulate miR-9 in vivo in an invertebrate model organism, the ascidian Ciona intestinalis, by microinjection of antisense molecules in the eggs. It is known that miR-9 is a well-conserved microRNA in bilaterians and we found that it is expressed in epidermal sensory neurons of the tail in the larva of C. intestinalis. Larvae developed from injected eggs showed a reduced differentiation of tail neurons, confirming the possibility to use peptide nucleic acid PNA to downregulate miRNA in a whole organism. By identifying putative targets of miR-9, we discuss the role of this miRNA in the development of the peripheral nervous system of ascidians.

scholarly journals Efficient inhibition of miR-155 function in vivo by peptide nucleic acids

2010 ◽  
Vol 38 (13) ◽  
pp. 4466-4475 ◽  
Author(s):  
Martin M. Fabani ◽  
Cei Abreu-Goodger ◽  
Donna Williams ◽  
Paul A. Lyons ◽  
Adrian G. Torres ◽  
...  
Keyword(s):  
Nucleic Acids ◽  
Peptide Nucleic Acids

scholarly journals miR-7 Knockdown by Peptide Nucleic Acids in the Ascidian Ciona intestinalis

2019 ◽  
Vol 20 (20) ◽  
pp. 5127 ◽  
Author(s):  
Silvia Mercurio ◽  
Silvia Cauteruccio ◽  
Raoul Manenti ◽  
Simona Candiani ◽  
Giorgio Scarì ◽  
...  
Keyword(s):  
Nucleic Acids ◽  
High Efficiency ◽  
Marine Invertebrates ◽  
Peptide Nucleic Acids ◽  
Ciona Intestinalis ◽  
Experimental Models ◽  
Sequence Specificity ◽  
Antisense Molecule ◽  
Ascidian Embryos

Peptide Nucleic Acids (PNAs) are synthetic mimics of natural oligonucleotides, which bind complementary DNA/RNA strands with high sequence specificity. They display numerous advantages, but in vivo applications are still rare. One of the main drawbacks of PNAs application is the poor cellular uptake that could be overcome by using experimental models, in which microinjection techniques allow direct delivery of molecules into eggs. Thus, in this communication, we investigated PNAs efficiency in miR-7 downregulation and compared its effects with those obtained with the commercially available antisense molecule, Antagomir (Dharmacon) in the ascidian Ciona intestinalis. Ascidians are marine invertebrates closely related to vertebrates, in which PNA techniques have not been applied yet. Our results suggested that anti-miR-7 PNAs were able to reach their specific targets in the developing ascidian embryos with high efficiency, as the same effects were obtained with both PNA and Antagomir. To the best of our knowledge, this is the first evidence that unmodified PNAs can be applied in in vivo knockdown strategies when directly injected into eggs.

Addressing the challenges of cellular delivery and bioavailability of peptide nucleic acids (PNA)

2005 ◽  
Vol 38 (4) ◽  
pp. 345-350 ◽  
Author(s):  
Peter E. Nielsen
Keyword(s):  
Nucleic Acids ◽  
Peptide Nucleic Acid ◽  
Peptide Nucleic Acids ◽  
Cell Penetrating Peptides ◽  
Endosomal Escape ◽  
Cellular Delivery ◽  
Peptide Conjugation ◽  
Cell Penetrating ◽  
Antisense Peptide

1. Introduction 3452. Peptide nucleic acid (PNA) 3463. ‘Cell penetrating peptides’ (CPPs) 3464. Endosomal escape 3475. Cellular delivery of PNA 3476.In vivobioavailability of PNA 3497. References 350Recent results on the cellular delivery of antisense peptide nucleic acids (PNA) via peptide conjugation is briefly discussed, in particular in the context of endosomal entrapment and escape.

scholarly journals Poly(Lactic-co-Glycolic Acid) Nanoparticle Delivery of Peptide Nucleic Acids In Vivo

2020 ◽  
pp. 261-281
Author(s):  
Stanley N. Oyaghire ◽  
Elias Quijano ◽  
Alexandra S. Piotrowski-Daspit ◽  
W. Mark Saltzman ◽  
Peter M. Glazer
Keyword(s):  
Nucleic Acids ◽  
Glycolic Acid ◽  
Peptide Nucleic Acids ◽  
Nanoparticle Delivery
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