Developing a SARS-CoV-2 Antigen Test Using Engineered Affinity Proteins

2021 ◽  
Author(s):  
Seunghyeon Kim ◽  
Emma Yee ◽  
Eric A. Miller ◽  
Yining Hao ◽  
Dousabel M. Y. Tay ◽  
...  
Keyword(s):  
Antigen Test ◽  
Affinity Proteins

Sputum und Urin-Pneumokokken-Antigen-Test in der Diagnostik der ambulant erworbenen Pneumokokken Pneumonie

Pneumologie ◽  
2008 ◽  
Vol 62 (S 2) ◽  
Author(s):  
S Krüger ◽  
N Suttorp ◽  
R Marre ◽  
K Richter ◽  
C Schumann ◽  
...  
Keyword(s):  
Antigen Test

PREVALENCE OF CERTAIN VIRAL DISEASES AND DIROFILARIA IMMITIS INFECTION IN STRAY DOGS IN BANGLADESH

2019 ◽  
Vol 1 (1) ◽  
Author(s):  
M. Tarafder
Keyword(s):  
Dirofilaria Immitis ◽  
Large Population ◽  
Zoonotic Diseases ◽  
Viral Diseases ◽  
Small Animals ◽  
Antigen Test ◽  
Stray Dogs ◽  
Negative Results ◽  
High Prevalence ◽  
Canine Influenza

Background: Stray and pet dogs constitute the dog population in Bangladesh without any established dog statistics. Diseases of dogs are not only associated with morbidity and mortality in dogs but also associated with human health problems as zoonotic diseases. Inland reports on the prevalence of dog diseases are limited in Bangladesh and there is need to investigate diseases in both stray and pet dog populations. Objectives: The main objective is to determine the prevalence of Canine distemper (CD), Canine adenovirus- 1 (CAV-1), CAV-2, Canine influenza (CI) and Dirofilaria immitis (Heart worm infection = HWI) infection in stray dogs in Bangladesh. Materials and Methods: Blood, ocular and nasal samples were collected from each of 30 randomly caught stray dogs (11 male and 19 female) and of different ages, including growing (n = 3) and adult (n = 27) dogs in the district of Mymensingh in Bangladesh from January to June 2010. These samples were tested by using Antigen Test Kits (RapiGEN Inc, Korea). Results: All the 30 stray dogs showed negative results to CD, CAV-1 and CAV-2, whereas an overall high prevalence of CI (4/30; 13.33%) and HWI (15/30; 50.0%) were recorded. The higher prevalence of CI was recorded in growing (n = 2/3; 66.67%) than adult (n = 2/27; 7.41%) but it did not differ significantly (p > 0.05) between male (n = 2/11; 18.18%) and female (n = 2/19; 10.53%) dogs. Significantly (p < 0.05) higher prevalence of D. immitis infection was recorded in adult (n = 15/27; 55.56%) than growing (0/3; 0.0%), male (n = 8/11; 72.73%) than female (n = 7/19; 36.84%) dogs. Conclusions: Further studies on CI and D. immitis are necessary on a large population of stray and pet small animals along with humans to ascertain their importance on health and zoonotic significance in Bangladesh. Key words: Viral diseases, Dirofilariasis, Stray dogs, Antigen test kits

Clinical Assessment of the Roche SARS-CoV-2 Rapid Antigen Test

2020 ◽  
Author(s):  
Giuseppe Lippi ◽  
Gianluca Gianfilippi ◽  
Damiano Bragantini ◽  
Brandon Henry ◽  
Gian Luca Salvagno
Keyword(s):  
Clinical Assessment ◽  
Antigen Test

Evaluation of Clinical Profile, Diagnostic Tests, And Prognosis of Cryptococcal Meningitis in HIV Infected Patients in Western India

2020 ◽  
Vol 20 ◽  
Author(s):  
Deepak Kumar ◽  
Suresh Bishnoi ◽  
Durga Shankar Meena ◽  
Gopal Krishana Bohra ◽  
Naresh Midha ◽  
...  
Keyword(s):  
Prognostic Factors ◽  
Clinical Features ◽  
Cryptococcal Meningitis ◽  
Fatal Outcome ◽  
Clinical Profile ◽  
Western India ◽  
Antigen Test ◽  
Cryptococcal Antigen ◽  
India Ink ◽  
Csf Examination

Background: Cryptococcal meningitis is a dreaded complication in HIV infected patients. It was associated with high mortality and morbidity before the advent of Highly Active Anti-Retroviral Therapy. A study was planned in our institute to evaluate the clinical profile, laboratory findings, and prognostic factors for survival in these patients. Settings and Design: A prospective hospital-based observational study, conducted in the Department of Medicine at a tertiary care centre in western Rajasthan. Method and Material: HIV infected patients presenting with clinical features of meningitis, and positive CSF culture for Cryptococcus were included in our study. All cases underwent detailed clinical history, physical examinations and relevant laboratory investigations including CD4 count and CSF examination. Results: 48 HIV infected cryptococcal meningitis patients were analysed, and the most common presenting clinical features were headache (85.42%), and fever (72.92%), followed by neck stiffness (62.50%). CSF examination was also done for Cryptococcal Antigen test which was reactive in all cases (100%), and India ink staining was positive in 43 cases (89.58%). Significant fatal outcome was associated with patients presenting with altered sensorium, loss of consciousness, cranial nerve palsy and CD4 cell count of less than 100. Similarly, on laboratory and imaging diagnosis, cryptococcal antigen test (>3+reactive), fundus examination (papilloedema) and abnormal CT/MRI brain imaging were associated with poor survival. Conclusion: Cryptococcal meningitis is a potentially lethal infection in immunocompromised individuals and should be diagnosed early with high clinical suspicion as around 10% of the cases may not be detected on India ink staining and a large proportion (75%) of cases may have normal imaging at initial evaluation. Early diagnosis, watchful eye on prognostic factors and treatment is vital to improve outcome in these patients.

scholarly journals Limits and Opportunities of SARS-CoV-2 Antigen Rapid Tests: An Experienced-Based Perspective

Pathogens ◽  
2021 ◽  
Vol 10 (1) ◽  
pp. 38
Author(s):  
Verena Schildgen ◽  
Sabrina Demuth ◽  
Jessica Lüsebrink ◽  
Oliver Schildgen
Keyword(s):  
Clinical Setting ◽  
Gold Standard ◽  
Antigen Test ◽  
Public Attention ◽  
High Expectations ◽  
Test Strategy ◽  
Rapid Tests ◽  
Pilot Sample ◽  
Lateral Flow Tests ◽  
Throat Washing

Background: Due to the steadily rising case numbers of SARS-CoV-2 infections worldwide, there is an increasing need for reliable rapid diagnostic devices in addition to existing gold standard PCR methods. Actually, public attention is focused on antigen assays including lateral flow tests (LFTs) as a diagnostic alternative. Therefore, different LFTs were analyzed regarding their performance in a clinical setting. Material and Methods: A pilot sample panel of 13 bronchoalveolar fluids (BALFs) and 60 throat washing (TW) samples with confirmed PCR results, as well as eight throat washes invalid by PCR, were tested with the BIOCREDIT test (RapiGEN), the PanbioTM assay (Abbott), and the SARS-CoV-2 rapid antigen test (Roche). Conclusion: The analyzed antigen test showed an interassay correlation of 27.4%, with overall specificities ranging from 19.4% to 87.1%, while sensitivities of the respective tests ranged between 33.3% and 88.1%. Because these assays did not entirely meet all high expectations, their benefit has to be carefully evaluated for the respective test strategy and setting.

scholarly journals Clinical evaluation of rapid point-of-care antigen tests for diagnosis of SARS-CoV-2 infection

2021 ◽  
Author(s):  
Johannes G. M. Koeleman ◽  
Henk Brand ◽  
Stijn J. de Man ◽  
David S. Y. Ong
Keyword(s):  
Health Care ◽  
Health Care Workers ◽  
Antigen Test ◽  
Duration Of Symptoms ◽  
Large Variability ◽  
Clinical Sensitivity ◽  
Care Workers ◽  
Upper Respiratory ◽  
Antigen Tests ◽  
Respiratory Specimens

AbstractThe RT-qPCR in respiratory specimens is the gold standard for diagnosing acute COVID-19 infections. However, this test takes considerable time before test results become available, thereby delaying patients from being diagnosed, treated, and isolated immediately. Rapid antigen tests could overcome this problem. In the first study, clinical performances of five rapid antigen tests were compared to RT-qPCR in upper respiratory specimens from 40 patients with positive and 40 with negative RTq-PCR results. In the second study, the rapid antigen test with one of the best test characteristics (Romed) was evaluated in a large prospective collection of upper respiratory specimens from 900 different COVID-19-suspected patients (300 emergency room patients, 300 nursing home patients, and 300 health care workers). Test specificities ranged from 87.5 to 100.0%, and test sensitivities from 55.0 to 80.0%. The clinical specificity of the Romed test was 99.8% (95% CI 98.9–100). Overall clinical sensitivity in the study population was 73.3% (95% CI 67.9–78.2), whereas sensitivity in the different patient groups varied from 65.3 to 86.7%. Sensitivity was 83.0 to 86.7% in patients with short duration of symptoms. In a population with a COVID-19 prevalence of 1%, the negative predictive value in all patients was 99.7%. There is a large variability in diagnostic performance between rapid antigen tests. The Romed rapid antigen test showed a good clinical performance in patients with high viral loads (RT-qPCR cycle threshold ≤30), which makes this antigen test suitable for rapid identification of COVID-19-infected health care workers and patients.

Assessment of Rapid Optimized 96-well Tray Flow Cytometric Crossmatch (Halifax-FCXM) with Luminex Single Antigen Test

2021 ◽  
Author(s):  
Jaeeun Yoo ◽  
Sangyoon Lee ◽  
Ho Won Lee ◽  
Soojung Lee ◽  
Jieun Choi ◽  
...  
Keyword(s):  
Antigen Test ◽  
Flow Cytometric ◽  
Single Antigen

scholarly journals Does the SARS-CoV-2 rapid antigen test result correlate with the viral culture result?

2021 ◽  
Author(s):  
Kei Yamamoto ◽  
Mami Nagashima ◽  
Isao Yoshida ◽  
Kenji Sadamasu ◽  
Masami Kurokawa ◽  
...  
Keyword(s):  
Antigen Test ◽  
Culture Result ◽  
Viral Culture ◽  
Test Result
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