The Polymeric Matrix Composition of Vibrio cholerae Biofilms Modulate Resistance to Silver Nanoparticles Prepared by Hydrothermal Synthesis

Author(s):  
Clémence Abriat ◽  
Olivier Gazil ◽  
Marie-Claude Heuzey ◽  
France Daigle ◽  
Nick Virgilio
RSC Advances ◽  
2015 ◽  
Vol 5 (106) ◽  
pp. 87151-87156 ◽  
Author(s):  
Yuhan Wu ◽  
Zhiqiang Wang ◽  
Shanshan Chen ◽  
Jianning Wu ◽  
Xuhong Guo ◽  
...  

In this work, we report a novel and facile one-step approach for synthesis of silver nanoparticles (Ag NPs) loaded on N-doped carbon (NC) composites.


2016 ◽  
Vol 63 (7) ◽  
pp. 627-635
Author(s):  
Abbas Jorsaraei Talar ◽  
Hamideh Ghasemi ◽  
Alimorad Rashidi ◽  
Saeed Khodabakhshi

2015 ◽  
Vol 13 (2) ◽  
pp. 391-403 ◽  
Author(s):  
Ghislain Djiokeng Paka ◽  
Sihem Doggui ◽  
Ahlem Zaghmi ◽  
Ramia Safar ◽  
Lé Dao ◽  
...  

2013 ◽  
Vol 85 ◽  
pp. 2-10 ◽  
Author(s):  
Gabriele Aksomaityte ◽  
Martyn Poliakoff ◽  
Edward Lester

Author(s):  
Luciano André Deitos Koslowski ◽  
André Lourenço Nogueira ◽  
Silvana Licodiedoff ◽  
Adrieny Taliny Comper ◽  
Marilena Valadares Folgueras

The importance of the preservation of water resources has resulted in the application of technologies such as nanostructured materials, which are able to minimize the impact associated with water contamination. This work evaluated the application of polyamide-66 (PA) pellets functionalized with silver nanoparticles (AgNPs) at polymer mass percentages of 0.05, 0.10 and 0.50% to disinfect of drinking water. Studies were carried out in three stages. The first stage was the synthesis of the silver nanoparticles by using silver nitrate as a metal precursor and sodium borohydrate as a reduction agent. The colloidal dispersion was characterized by UV-Vis spectrophotometry and transmission electron microscopy (MET). Afterwards, the nanostructures were incorporated into a polyamide-66 polymeric matrix. In the second stage, the silver ions leached from the polymer matrix in the water after a three-hour period were quantified in order to evaluate the limit established by Conama Resolution 357/2005, which imposes a concentration limit of 0.010 mg L-1. The best results were obtained with the application of 0.05% AgNPs in the polymeric matrix, yielding an average concentration of silver ions lixiviated of 0.008 mg L-1. The last step comprised the quantification of the antibacterial activity of the polymer matrix containing 0.05% of AgNPs against the microorganism E. coli using the Standard Test Method for Determining the Antimicrobial Agents Under Dynamic Contact Conditions. The samples containing 0.05% of AgNPs exhibited an antibacterial reduction of 97.89% after 24 h of incubation under stirring at room temperature (25°C).


2021 ◽  
Author(s):  
Praveen K. Singh ◽  
Daniel K.H. Rode ◽  
Pauline Buffard ◽  
Kazuki Nosho ◽  
Miriam Bayer ◽  
...  

The extracellular matrix is a defining feature of bacterial biofilms and provides structural stability to the community by binding cells to the surface and to each other. Transitions between bacterial biofilm initiation, growth, and dispersion require different regulatory programs, all of which result in modifications to the extracellular matrix composition, abundance, or functionality. However, the mechanisms by which individual cells in biofilms disengage from the matrix to enable their departure during biofilm dispersal are unclear. Here, we investigated active biofilm dispersal of Vibrio cholerae during nutrient starvation, resulting in the discovery of the conserved Vibrio biofilm dispersal regulator VbdR. We show that VbdR triggers biofilm dispersal by controlling cellular release from the biofilm matrix, which is achieved by inducing the retraction of the mannose-sensitive hemagglutinin (MSHA) type IV pili and the expression of a matrix protease IvaP. We further show that MSHA pili have numerous binding partners in the matrix and that the joint effect of MSHA pilus retraction and IvaP activity is necessary and sufficient for causing biofilm dispersal. These results highlight the crucial role of type IV pilus dynamics during biofilm dispersal and provide a new target for controlling V. cholerae biofilm abundance through the induction and manipulation of biofilm dispersal.


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