scholarly journals Direct Immobilization of Engineered Nanobodies on Gold Sensors

2021 ◽  
Author(s):  
Bárbara Simões ◽  
Wanda J. Guedens ◽  
Charlie Keene ◽  
Karina Kubiak-Ossowska ◽  
Paul Mulheran ◽  
...  
Keyword(s):  
Direct Immobilization

Direct immobilization of DNA on diamond-like carbon nanodots

2006 ◽  
Vol 17 (8) ◽  
pp. 2004-2007 ◽  
Author(s):  
T Djenizian ◽  
E Balaur ◽  
P Schmuki
Keyword(s):  
Carbon Nanodots ◽  
Diamond Like Carbon ◽  
Direct Immobilization

Highly reusable TiO 2 nanoparticle photocatalyst by direct immobilization on steel mesh via PVDF coating, electrospraying, and thermal fixation

2016 ◽  
Vol 306 ◽  
pp. 344-351 ◽  
Author(s):  
Subramaniyan Ramasundaram ◽  
Mingizem Gashaw Seid ◽  
Jae Wan Choe ◽  
Eun-Ju Kim ◽  
Yun Chul Chung ◽  
...  
Keyword(s):  
Steel Mesh ◽  
Direct Immobilization

scholarly journals Direct Immobilization of Gadolinium Complex on Silica Particles and Their MRI Properties

2015 ◽  
Vol 13 (0) ◽  
pp. 42-46 ◽  
Author(s):  
Yoshio Kobayashi ◽  
Kyosuke Shibuya ◽  
Tomohiko Nakagawa ◽  
Yohsuke Kubota ◽  
Kohsuke Gonda ◽  
...  
Keyword(s):  
Silica Particles ◽  
Gadolinium Complex ◽  
Direct Immobilization

scholarly journals Immobilization Techniques for Aptamers on Gold Electrodes for the Electrochemical Detection of Proteins: A Review

Biosensors ◽  
2020 ◽  
Vol 10 (5) ◽  
pp. 45
Author(s):  
Franziska V. Oberhaus ◽  
Dieter Frense ◽  
Dieter Beckmann
Keyword(s):  
Electrochemical Detection ◽  
Gold Electrodes ◽  
Dna Nanostructures ◽  
Profound Knowledge ◽  
Low Concentrations ◽  
Reduced Graphene ◽  
Highly Sensitive ◽  
The Subject ◽  
Immobilization Techniques ◽  
Direct Immobilization

The development of reliable biosensing platforms plays a key role in the detection of proteins in clinically and environmentally derived samples for diagnostics, as well as for process monitoring in biotechnological productions. For this purpose, the biosensor has to be stable and reproducible, and highly sensitive to detect potentially extremely low concentrations and prevent the nonspecific binding of interfering compounds. In this review, we present an overview of recently published (2017–2019) immobilization techniques for aptamers on gold electrodes for the electrochemical detection of proteins. These include the direct immobilization of thiolated aptamers and the utilization of short linkers, streptavidin/biotin interaction, as well as DNA nanostructures and reduced graphene oxide as immobilization platforms. Applied strategies for signal amplification and the prevention of biofouling are additionally discussed, as they play a crucial role in the design of biosensors. While a wide variety of amplification strategies are already available, future investigations should aim to establish suitable antifouling strategies that are compatible with electrochemical measurements. The focus of our review lies on the detailed discussion of the underlying principles and the presentation of utilized chemical protocols in order to provide the reader with promising ideas and profound knowledge of the subject, as well as an update on recent discoveries and achievements.

Direct immobilization of milk xanthine oxidase in milk protein with and without addition of gelatin

1979 ◽  
Vol 1 (3) ◽  
pp. 133-138 ◽  
Author(s):  
J. Tramper ◽  
H. C. van der Plas ◽  
F. Müller
Keyword(s):  
Xanthine Oxidase ◽  
Milk Protein ◽  
Direct Immobilization

Direct immobilization of DNA on partially functionalized diamond surface

2006 ◽  
Vol 950 ◽  
Author(s):  
Jung-Hoon Yang ◽  
Kwang-Soup Song ◽  
Shouma Kuga ◽  
Hiroshi Kawarada
Keyword(s):  
Epifluorescence Microscopy ◽  
Diamond Surface ◽  
Amino Groups ◽  
Simple Process ◽  
Specific Hybridization ◽  
Target Dna ◽  
Linker Molecules ◽  
Direct Immobilization

ABSTRACTAmino groups were functionalized directly on the diamond surface after treating oxidation and fluorination for detection of DNAs, respectively. For simple process, immobilization of probe DNAs was carried out directly on the partially aminated diamond without linker molecules. After fabricating micropatterned diamond, specific hybridization with Cy-5 labeled target DNA at a concentration of 100 nM could be clearly detected on H-terminated, partially O-terminated, and partially F-terminated diamonds, respectively. The hybridization intensities determined by epifluorescence microscopy were compared and analyzed.

Direct immobilization of sugar probes on bovine serum albumin-coated gold substrate for the development of glycan biosensors

2019 ◽  
Vol 14 (1) ◽  
pp. 011003
Author(s):  
Shun Tao ◽  
Wen-Wen Wang ◽  
Li-Qiang Chu ◽  
Tian-Wei Jia ◽  
Yang Yang
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Gold Substrate ◽  
Direct Immobilization
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