Immunostimulatory AIE Dots for Live-Cell Imaging and Drug Delivery

2021 ◽  
Author(s):  
Peilin Gu ◽  
Bin Chen ◽  
Tingting Zhai ◽  
Qian Li ◽  
Xiaolei Zuo ◽  
...  
Keyword(s):  
Drug Delivery ◽  
Live Cell Imaging ◽  
Cell Imaging ◽  
Live Cell

scholarly journals Time-lapse live cell imaging to monitor doxorubicin release from DNA origami nanostructures

2018 ◽  
Vol 6 (11) ◽  
pp. 1605-1612 ◽  
Author(s):  
Yun Zeng ◽  
Jiajun Liu ◽  
Shuo Yang ◽  
Wenyan Liu ◽  
Liang Xu ◽  
...  
Keyword(s):  
Drug Delivery ◽  
Live Cell Imaging ◽  
Cell Imaging ◽  
Time Lapse ◽  
Dna Origami ◽  
Live Cell ◽  
Doxorubicin Release

DNA origami nanostructures can serve as a promising carrier for drug delivery due to the outstanding programmability and biocompatibility.

scholarly journals Live-Cell Imaging of Colloidal Mesoporous Silica Nanoparticles for Drug Delivery: Drug Loading, Pore Sealing and Controlled Release

2011 ◽  
Vol 100 (3) ◽  
pp. 600a
Author(s):  
Anna M. Sauer ◽  
Axel Schlossbauer ◽  
Valentina Cauda ◽  
Hanna Engelke ◽  
Christian Argyo ◽  
...  
Keyword(s):  
Drug Delivery ◽  
Controlled Release ◽  
Mesoporous Silica ◽  
Silica Nanoparticles ◽  
Live Cell Imaging ◽  
Cell Imaging ◽  
Mesoporous Silica Nanoparticles ◽  
Drug Loading ◽  
Live Cell ◽  
Pore Sealing

Polyhedral borane derivatives: Unique and versatile structural motifs

2012 ◽  
Vol 84 (11) ◽  
pp. 2279-2288 ◽  
Author(s):  
M. Frederick Hawthorne ◽  
Alexei Pushechnikov
Keyword(s):  
Drug Delivery ◽  
Aromatic Compounds ◽  
Live Cell Imaging ◽  
Cell Imaging ◽  
Live Cell ◽  
Molecular Volume ◽  
Structural Motifs ◽  
Modular Assembly ◽  
Drug Delivery Vehicles ◽  
Delivery Vehicles

Compounds with polyhedral borane moieties have demonstrated numerous unique properties for a variety of applications, including nanoelectronics, drug delivery vehicles, and live cell imaging. Polyhedral boranes are good pharmacophore analogs of carbocycles because polyhedral boranes are inherently insensitive to many undesirable enzymatic metabolic transformations typical for a majority of aromatic compounds. The defined shape, low molecular volume, and high 3D symmetry of the surface are useful for the application of the polyhedral borane scaffolds as universal and convenient spacers for the modular assembly approach with a controllable predisposition of peripheral groups.

Role of Endosomal Escape for Disulfide-Based Drug Delivery from Colloidal Mesoporous Silica Evaluated by Live-Cell Imaging

Nano Letters ◽  
2010 ◽  
Vol 10 (9) ◽  
pp. 3684-3691 ◽  
Author(s):  
Anna M. Sauer ◽  
Axel Schlossbauer ◽  
Nadia Ruthardt ◽  
Valentina Cauda ◽  
Thomas Bein ◽  
...  
Keyword(s):  
Drug Delivery ◽  
Mesoporous Silica ◽  
Live Cell Imaging ◽  
Cell Imaging ◽  
Live Cell ◽  
Endosomal Escape

scholarly journals Development and In Vitro Characterization of Transferrin-Decorated Nanoemulsion Utilizing Hydrophobic Ion Pairing for Targeted Cellular Uptake

2021 ◽  
Author(s):  
Sergey Zaichik ◽  
Christian Steinbring ◽  
Julian David Friedl ◽  
Andreas Bernkop-Schnürch
Keyword(s):  
Drug Delivery ◽  
Flow Cytometry ◽  
Zeta Potential ◽  
Droplet Size ◽  
Cellular Uptake ◽  
Live Cell Imaging ◽  
Cell Imaging ◽  
Ion Pairing ◽  
Live Cell ◽  
Hydrophobic Ion Pairing

Abstract Purpose The aim of this study was to develop transferrin-conjugated nanoemulsions utilizing hydrophobic ion pairing for a targeted cellular uptake. Methods In the lipophilic phase of nanoemulsion composed of 60% oleic acid, 30% Capmul MCM EP and 10% Span 85, 1% cetyltrimethylammonium bromide (CTAB) and 3% phosphatidic acid (PA) were incorporated. After emulsification, the resulting droplets were decorated with human protein transferrin via hydrophobic ion pairing with PA and characterized regarding droplet size and zeta potential. Subsequently, cellular uptake of transferrin-conjugated nanoemulsion was investigated on Caco-2 and HeLa cell lines and determined by flow cytometry, cell lysis method and live cell imaging using confocal laser scanning microscopy. Results The nanoemulsion showed a droplet size of 123.03 ± 2.1 nm and zeta potential of − 54.5 mV that changed because of the surface decoration with transferrin to 182.7 ± 0.2 and + 30.2 mV, respectively. Within the uptake studies utilizing flow cytometry, transferrin-conjugated nanoemulsion showed a 5.2-fold higher uptake in Caco-2 cells and twofold improvement in case of HeLa cells compared with unmodified formulation. The outcome was confirmed visually via live cell imaging. Conclusion According to the results, transferrin-conjugated nanoemulsion might be considered as a promising drug delivery system for a selective receptor-mediated drug delivery.

Sign in / Sign up

Export Citation Format

Share Document