Elimination of Uremic Toxins by Functionalized Graphene-Based Composite Beads for Direct Hemoperfusion

2021 ◽  
Vol 13 (5) ◽  
pp. 5955-5965
Author(s):  
Abhishek Tyagi ◽  
Yik Wong Ng ◽  
Mohsen Tamtaji ◽  
Irfan Haider Abidi ◽  
Jingwei Li ◽  
...  
Keyword(s):  
Uremic Toxins ◽  
Functionalized Graphene ◽  
Direct Hemoperfusion ◽  
Composite Beads

Adsorption of Protein-Bound Uremic Toxins Using Activated Carbon through Direct Hemoperfusion in vitro

2019 ◽  
Vol 48 (3) ◽  
pp. 215-222 ◽  
Author(s):  
Suguru Yamamoto ◽  
Toru Ito ◽  
Mami Sato ◽  
Shin Goto ◽  
Junichiro J. Kazama ◽  
...  
Keyword(s):  
Activated Carbon ◽  
Indole Acetic Acid ◽  
Reduction Rate ◽  
Uremic Toxins ◽  
Dependent Manner ◽  
Bovine Blood ◽  
Dialysis Treatment ◽  
Direct Hemoperfusion ◽  
Dose Dependent

Background/Aims: Accumulation of protein-bound uremic toxins (PBUTs) is associated with mortality due to various systemic disorders in patients with chronic kidney disease (CKD), especially in those undergoing dialysis treatment. The clinical outcomes of such patients could be improved by removing sufficient amounts of PBUTs; however, conventional dialysis lacks this ability. We examined the efficacy of activated carbon in adsorbing circulating PBUTs through direct hemoperfusion (DHP) in vitro. Methods: An in vitro blood circulating system was constructed with 8.5 mL blood circulating around a column containing activated carbon (50, 100, or 200 mg). Bovine blood containing a kind of PBUT (at the same concentration as that found in the blood of dialysis patients) and blood from hemodialysis patients (n = 8) were used. After circulation for the designated amount of time, sera were collected and the levels of PBUTs, including indoxyl sulfate (IS), p-cresyl sulfate, indole acetic acid (IAA), phenyl sulfate, and hippuric acid, were analyzed with mass spectrometry. Results: Activated carbon decreased the PBUT level in bovine blood in a dose-dependent manner (e.g., reduction rate of IS: 67.9 ± 3.8, 83.3 ± 1.9, and 94.5 ± 1.1% after 60-min circulation in columns containing 50, 100, and 200 mg activated carbon respectively). IS, PCS, and IAA were dramatically adsorbed by activated carbon from the blood of patients undergoing hemodialysis (pre vs. post 240-min reaction: IS 2.835 ± 0.876 vs. 0.455 ± 0.108 mg/dL [p < 0.01], PCS 3.208 ± 2.876 vs. 0.768 ± 0.632 mg/dL [p < 0.01], IAA 0.082 ± 0.045 vs. 0.016 ± 0.005 mg/dL [p < 0.01]). Conclusion: Activated carbon effectively adsorbed blood PBUTs in vitro. DHP with activated carbon could be a promising strategy for removing circulating PBUTs from the blood of patients with CKD.

Adsorption of Protein-Bound Uremic Toxins Through Direct Hemoperfusion With Hexadecyl-Immobilized Cellulose Beads in Patients Undergoing Hemodialysis

2017 ◽  
Vol 42 (1) ◽  
pp. 88-93 ◽  
Author(s):  
Suguru Yamamoto ◽  
Mami Sato ◽  
Yoko Sato ◽  
Takuya Wakamatsu ◽  
Yoshimitsu Takahashi ◽  
...  
Keyword(s):  
Uremic Toxins ◽  
Direct Hemoperfusion

Uremic toxins

1970 ◽  
Vol 126 (5) ◽  
pp. 843-845 ◽  
Author(s):  
P. L. Balestri
Keyword(s):  
Uremic Toxins

Uremic toxins and platelet function

1970 ◽  
Vol 126 (5) ◽  
pp. 823-826 ◽  
Author(s):  
H. I. Horowitz
Keyword(s):  
Platelet Function ◽  
Uremic Toxins

EFFICIENCY OF NEW HEMOSORBENT IN ACUTE AMITRIPTYLINE AND CYCLODOL POISONING

2020 ◽  
pp. 14-18
Author(s):  
A. M. Fomin
Keyword(s):  
Clinical Effect ◽  
High Efficiency ◽  
Initial Level ◽  
Synthetic Polymer ◽  
Complex Treatment ◽  
Direct Hemoperfusion ◽  
Selective Sorption ◽  
Positive Clinical Effect ◽  
Before And After

A complex treatment of a patient with acute amitriptyline and cyclodol poisoning using enterosorption, intestinal lavage and hemosorption on a new column with a synthetic sorbent is presented. A two-layer synthetic polymer column developed for the selective sorption of cytokines by direct hemoperfusion was used for hemosorption. Quantitative determinations of amitriptyline and cyclodol levels before and after the column, as well as before and after hemosorption have showed high efficiency of the sorbent to remove the toxicant from the blood. The use of 6-hour hemosorption allowed to reduce the level of amitriptyline from the initial level by more than 4 times and the level of cyclodol - by more than 3 times to therapeutic levels and to obtain a pronounced positive clinical effect in the complex treatment of a patient with severe poisoning.

Adsorption-Based Strategies for Removing Uremic Toxins from Blood

2020 ◽  
Author(s):  
Yuhao Ma ◽  
Shuhui Li ◽  
Marcello Tonelli ◽  
Larry Unsworth
Keyword(s):  
Uremic Toxins

Polymer Functionalized Graphene Oxide: A Versatile Nanoplatform for Drug/Gene Delivery

2015 ◽  
Vol 19 (18) ◽  
pp. 1828-1837 ◽  
Author(s):  
George V. Theodosopoulos ◽  
Panayiotis Bilalis ◽  
Georgios Sakellariou
Keyword(s):  
Graphene Oxide ◽  
Gene Delivery ◽  
Functionalized Graphene ◽  
Functionalized Graphene Oxide

Pilot Study of Probiotic Supplementation on Uremic Toxicity and Inflammatory Cytokines in Chronic Kidney Patients

2020 ◽  
Vol 16 (4) ◽  
pp. 470-480
Author(s):  
Cristina T. Roth-Stefanski ◽  
Carla Dolenga ◽  
Lia S. Nakao ◽  
Roberto Pecoits-Filho ◽  
Thyago P. de Moraes ◽  
...  
Keyword(s):  
Pilot Study ◽  
Inflammatory Cytokines ◽  
Lactobacillus Acidophilus ◽  
Serum Levels ◽  
Uremic Toxins ◽  
Indoxyl Sulfate ◽  
Bacterial Metabolism ◽  
Probiotic Supplementation ◽  
Primary Endpoints ◽  
Inflammatory Profile

Background: Bacterial metabolism contributes to the generation of uremic toxins in patients with chronic kidney disease (CKD). It has been investigated the use of probiotics in the reduction of uremic toxins intestinal production. Objective: The aim of this pilot study was to evaluate the effect of probiotic supplementation on reducing the production of uremic toxins and the inflammatory profile of CKD patients. Methods: We performed a randomized, blind, placebo-controlled, crossover study on patients with CKD stages 3 and 4. The intervention was a probiotic formulation composed of Lactobacillus acidophilus strains given orally three times a day for 3 months. Changes in uremic toxins (p-Cresylsulfate and Indoxyl Sulfate) and serum inflammatory cytokines were the primary endpoints. Results: Of the 44 patients randomized, 25 completed the study (mean age 51 ± 9.34, 64% female, mean eGFR 36 ± 14.26 mL/min/1.73m², mean BMI 28.5 ± 5.75 kg/m²). At 3 months, there were no significant changes in any of the studied biomarkers including p-cresylsulfate (p = 0.57), Indoxyl sulfate (p = 0.08) and interleukin-6 (p = 0.55). Conclusion: Lactobacillus acidophilus strains given as probiotic were not able to reduce serum levels of uremic toxins and biomarkers of inflammation in CKD patients in stage 3 and 4.

Sign in / Sign up

Export Citation Format

Share Document