Acidic Polysaccharides as Green Alternatives for Barite Scale Dissolution

2020 ◽  
Vol 12 (49) ◽  
pp. 55434-55443
Author(s):  
Ricardo D. Sosa ◽  
Xi Geng ◽  
Ankur Agarwal ◽  
Jeremy C. Palmer ◽  
Jacinta C. Conrad ◽  
...  
Keyword(s):  
Acidic Polysaccharides

scholarly journals Histochemical and morphological features of biopsied and stripped oocytes from the Brazilian endangered teleost pirapitinga, Brycon nattereri (Characiformes)

Zygote ◽  
2014 ◽  
Vol 23 (3) ◽  
pp. 360-366 ◽  
Author(s):  
Alexandre N. Maria ◽  
Laura H. Orfão ◽  
Elizete Rizzo ◽  
Alexandre Ninhaus-Silveira ◽  
Ana T.M. Viveiros
Keyword(s):  
Pituitary Extract ◽  
Follicular Cells ◽  
Animal Pole ◽  
Essential Information ◽  
Zona Radiata ◽  
Oocyte Morphology ◽  
Acidic Polysaccharides ◽  
Mean Diameter ◽  
Pore Canals ◽  
Scanning Electron

SummaryThe aim of the present study was to characterize biopsied and stripped oocytes from the Brazilian endangered teleost pirapitinga (Brycon nattereri) using histochemical and morphological analyses. Biopsied oocytes had a mean diameter of 2.225 mm (modal diameter: 2.312 mm), complete vitellogenesis and a central or slightly eccentric nucleus. Neutral polysaccharides were detected in the follicular cells, zona radiata and yolk globules, while acidic polysaccharides were detected in the follicular cells and cortical alveoli. Ten out of the 19 females treated with two doses of carp pituitary extract (cPE) released oocytes, which were also analysed. Stripping occurred 292 ± 39 degree-hours after the second dose of cPE and led to a mean spawning weight of 36.2 g, 10% spawning index, 241 oocytes/g of ova, 8222 oocytes/female and 23 oocytes/g of body weight. Stripped oocytes had a mean diameter of 2.33 mm and a mode at 2.375 mm, were weakly adhesive and coloration ranged from wine to brown. Under scanning electron microscopy, stripped oocytes exhibited a single funnel-shaped micropyle located at the animal pole and a zona radiata that measured 7.7 μm in thickness with eight pore canals/μm2. Oocyte morphology in Brycon nattereri is similar to that found in other species of the genus, except for the larger size and weaker adhesiveness. These findings provide essential information for a better understanding of the reproductive biology of B. nattereri and the establishment of conservation measures for this threatened species.

Anatomy of the Unpollinated and Pollinated Watermelon Stigma

1982 ◽  
Vol 54 (1) ◽  
pp. 341-355
Author(s):  
M. SEDGLEY
Keyword(s):  
Electron Microscopy ◽  
Endoplasmic Reticulum ◽  
Light And Electron Microscopy ◽  
Freeze Fracture ◽  
Pollen Grain ◽  
Acidic Polysaccharides ◽  
Before And After ◽  
Em Autoradiography ◽  
Pollen Grain Wall ◽  
Stigma Secretion

The structure of the watermelon stigma before and after pollination was studied using light and electron microscopy, freeze-fracture and autoradiography. The wall thickenings of the papilla transfer cells contained callose and their presence prior to pollination was confirmed using EM-autoradiography, freeze-fracture and fixation. No further callose thickenings were produced following pollination. Pollination resulted in a rapid increase in aqueous stigma secretion and localized disruption of the cuticle, which appeared to remain on the surface of the secretion. Autolysis of the papilla cells, which had commenced prior to pollination, was accelerated and appeared to take place via cup-shaped vacuoles developed from distended endoplasmic reticulum. The reaction was localized to the papilla cells adjacent to the pollen tube only. Both pollen-grain wall and stigma secretion contained proteins, carbohydrates, acidic polysaccharides, lipids and phenolics.

Electrophoretic behavior and size distribution of the acidic polysaccharides produced by the bacteriaBradyrhizobium (Chamaecytisus) strain BGA-1 andBradyrhizobium japonicum USDA 110

1998 ◽  
Vol 19 (15) ◽  
pp. 2621-2624 ◽  
Author(s):  
Ana R. Díaz-Marrero ◽  
Mónica Santamaria ◽  
Ana Poveda ◽  
Jesus Jiménez-Barbero ◽  
Javier Corzo
Keyword(s):  
Size Distribution ◽  
Electrophoretic Behavior ◽  
Acidic Polysaccharides

scholarly journals Extraction, Structural Characterization, and Anti-Hepatocellular Carcinoma Activity of Polysaccharides From Panax ginseng Meyer

2021 ◽  
Vol 11 ◽  
Author(s):  
Hui Jia ◽  
Bin Zhao ◽  
Fangfang Zhang ◽  
Ramesh Kumar Santhanam ◽  
Xinying Wang ◽  
...  
Keyword(s):  
Hepatocellular Carcinoma ◽  
Hepg2 Cells ◽  
Cytotoxic Effect ◽  
Structural Characteristics ◽  
Gel Permeation Chromatography ◽  
Salting Out ◽  
Gel Permeation ◽  
Acidic Polysaccharides ◽  
Hepg2 Cell Lines ◽  
First Time

Polysaccharides are the main active ingredients of ginseng. To extract the most effective polysaccharides against hepatocellular carcinoma (HCC), we isolated and characterized the polysaccharides from the mountain cultivated ginseng (MCG) and compared their composition and cytotoxic effect with cultivated ginseng (CG) polysaccharide against HepG2 cell lines for the first time. MCG polysaccharides and CG polysaccharides were fractionated into two fractions such as MTPS-1, MTPS-2 and CTPS-1, CTPS-2 by salting out, respectively. Compared to CG, MCG possessed appreciable cytotoxic effect against HepG2 cells among that MTPS-1 possess fortified effect. Then, MTPS-1 was selected for further isolation process and seven acidic polysaccharides (MCGP-1–MCGP-7) were obtained using ethanol precipitation, ion-exchange, and gel permeation chromatography techniques. Structural characteristics of the polysaccharides (MCGP-1–MCGP-7) were done by adapting methylation/GC-MS and NMR analysis. Overall, MCGP-3 polysaccharide was found to possess significant cytotoxic effect against HepG2 cells with the IC50 value.

Ruthenium-Red-Induced Cell Agglutination and Surface Glycoprotein and Muco-Polysaccharide

1973 ◽  
Vol 13 (3) ◽  
pp. 901-911
Author(s):  
KOZO UTSUMI ◽  
TAKUZO ODA
Keyword(s):  
Sialic Acid ◽  
Heparan Sulphate ◽  
Ruthenium Red ◽  
The Other ◽  
Surface Glycoprotein ◽  
Ascites Hepatoma ◽  
Cell Agglutination ◽  
Acidic Polysaccharides ◽  
Binding Substance ◽  
Papain Digestion

Ruthenium red induces the agglutination of ascites hepatoma cells. The agglutination was inhibited by acidic polysaccharides but not by haptenic inhibitors of some plant agglutinins. The agglutination was also abolished by treatment of the cells with a low concentration of papain. Papain digestion causes a decrease in the number of microvilli on the cell surface and the release of carbohydrate-containing red-binding substance. One of them is a heparan sulphate-like mucopolysaccharide and the other is a glycoprotein which contains sialic acid. The ruthenium red-binding fractions inhibit the cell agglutination induced by ruthenium red.

scholarly journals Chromatography of Acidic Polysaccharides on Deae-Cellulose

1962 ◽  
Vol 15 (4) ◽  
pp. 787 ◽  
Author(s):  
MA Jermyn
Keyword(s):  
Sugar Beet ◽  
Deae Cellulose ◽  
Alkaline Ph ◽  
Ion Exchangers ◽  
Pectic Acid ◽  
Acidic Polysaccharides ◽  
Borate Systems

The chromatography of polysaccharides on resinous ion-exchangers has been reported by Steiner, Neukom, and Deuel (1958). Neukom et al. (1960) later reported the chromatography of a variety of wheat and sugar-beet polysaccharides on DEAEcellulose. Only "neutral" polysaccharides could be eluted at pH 6-8 by increasing buffer and salt concentrations; acid polysaccharides, e.g. pectic acid, were eluted by various strengths of alkali. Neutral polysaccharides could also be hromatographed at a somewhat alkaline pH by working with borate systems, which give charged complexes with many carbohydrates.

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