scholarly journals Spatial and Temporal Control of CRISPR-Cas9-Mediated Gene Editing Delivered via a Light-Triggered Liposome System

2020 ◽  
Vol 12 (47) ◽  
pp. 52433-52444
Author(s):  
Yagiz Alp Aksoy ◽  
Biyao Yang ◽  
Wenjie Chen ◽  
Tzongtyng Hung ◽  
Rhiannon P. Kuchel ◽  
...  
Keyword(s):  
Gene Editing ◽  
Temporal Control ◽  
Liposome System

scholarly journals Improving Gene Editing Outcomes in Human Hematopoietic Stem and Progenitor Cells by Temporal Control of DNA Repair

Stem Cells ◽  
2018 ◽  
Vol 37 (2) ◽  
pp. 284-294 ◽  
Author(s):  
Anastasia Lomova ◽  
Danielle N. Clark ◽  
Beatriz Campo-Fernandez ◽  
Carmen Flores-Bjurström ◽  
Michael L. Kaufman ◽  
...  
Keyword(s):  
Dna Repair ◽  
Progenitor Cells ◽  
Gene Editing ◽  
Temporal Control ◽  
Hematopoietic Stem ◽  
Stem And Progenitor Cells

scholarly journals Spatial and temporal control of CRISPR/Cas9-mediated gene editing delivered via a light-triggered liposome system

2019 ◽  
Author(s):  
Yagiz Alp Aksoy ◽  
Wenjie Chen ◽  
Ewa M Goldys ◽  
Wei Deng
Keyword(s):  
Gene Editing ◽  
Transfection Efficiency ◽  
Hek293 Cells ◽  
Temporal Control ◽  
Reporter System ◽  
Specific Sequence ◽  
Egfp Gene ◽  
Spatio Temporal

ABSTRACTThe CRISPR-Cas9 and related systems offer a unique genome editing tool allowing facile and efficient introduction of heritable and locus-specific sequence modifications in the genome. Despite its molecular precision, temporal and spatial control of gene editing with CRISPR-Cas9 system is very limited. We developed a light-sensitive liposome delivery system that offers a high degree of spatial and temporal control of gene editing with CRISPR/Cas9 system. We demonstrated its high transfection efficiency, by assessing the targeted knockout of eGFP gene in human HEK293 cells (52.8% knockout). We further validated our results at a single-cell resolution using an in vivo eGFP reporter system in zebrafish (77% knockout). To the best of our knowledge we reported the first proof-of-concept of spatio-temporal control of CRISPR/Cas9 by using light-triggered liposomes in both in vitro and in vivo environment.

Temporal Control of Mental States in Pigeons

2007 ◽  
Author(s):  
Robert G. Cook ◽  
Hara A. Rosen
Keyword(s):  
Mental States ◽  
Temporal Control

Application of Gene Editing Technology on Germplasm Resources

2018 ◽  
Vol 20 (6) ◽  
pp. 82
Author(s):  
Hongbing Han ◽  
Kabin Xie ◽  
Gang Cao ◽  
Jianhui Tian ◽  
Dong Wang
Keyword(s):  
Gene Editing ◽  
Germplasm Resources
Sign in / Sign up

Export Citation Format

Share Document