scholarly journals Hierarchically Imprinted Polymer for Peptide Tag Recognition Based on an Oriented Surface Epitope Approach

2020 ◽  
Vol 12 (43) ◽  
pp. 49111-49121
Author(s):  
Lidia N. Gómez-Arribas ◽  
María del Mar Darder ◽  
Nuria García ◽  
Yoel Rodriguez ◽  
Javier L. Urraca ◽  
...  
Keyword(s):  
Imprinted Polymer ◽  
Oriented Surface ◽  
Epitope Approach ◽  
Peptide Tag

Selective Peptide Recognition With Molecularly Imprinted Polymers in Designing New Biomedical Devices

2006 ◽  
Author(s):  
U. Vitale ◽  
A. Rechichi ◽  
M. D’Alonzo ◽  
C. Cristallini ◽  
N. Barbani ◽  
...  
Keyword(s):  
Conformational Changes ◽  
Peptide Sequence ◽  
Imprinted Polymer ◽  
Molecularly Imprinted ◽  
Pentaerythritol Triacrylate ◽  
Ft Ir ◽  
Epitope Approach ◽  
Cell Adhesion Proteins ◽  
Short Peptide Sequence ◽  
Standard Techniques

Molecular imprinting is a technique for the synthesis of polymers capable to bind selectively specific molecules. The imprinting of large proteins, like cell adhesion proteins or cell receptors, can lead to important and innovative biomedical applications. However such molecules show such important conformational changes in the polymerisation environment that the recognition sites are poorly specific. The “epitope approach” can overcome this limit by adopting, as template, a stable short peptide sequence representative of an accessible fragment of a larger protein. The resulting imprinted polymer can recognize both the template and the whole molecule thanks to the specific cavities for the epitope. In this work two molecularly imprinted polymer formulations (macroporous monolith and nanospheres) were obtained with the protected peptides Z-Thr-Ala-Ala-OMe, as template, and Z-Thr-Ile-Leu-OMe, as analogue for the selectivity evaluation, the methacrylic acid, as functional monomer, the trimethylolpropane trimethacrylate and pentaerythritol triacrylate, as cross-linkers. Polymers were synthesized by precipitation polymerisation in acetonitrile at 60 °C, thermally initiated with azobisisobutyronitrile. All polymers were characterized by the standard techniques SEM, FT-IR, and TGA. The supernatants from the polymerisation and the rebinding solutions were analysed by HPLC. The higher cross-linked polymers retained about the 70% of the template, against about the 20% for the lower ones. The extracted template amount and the rebinding capacity decreased with the cross-linking degree, while the selectivity showed the opposite behaviour. The pentaerythritol triacrylate cross-linked polymers showed the best recognition (MIP 2−, α = 1.71) and selectivity (MIP 2+, α′ = 5.58) capabilities.

Targeted imaging and targeted therapy of breast cancer cells via fluorescent double template-imprinted polymer coated silicon nanoparticles by an epitope approach

Nanoscale ◽  
2019 ◽  
Vol 11 (36) ◽  
pp. 17018-17030 ◽  
Author(s):  
Hai-Yan Wang ◽  
Pei-Pei Cao ◽  
Zheng-Ying He ◽  
Xi-Wen He ◽  
Wen-You Li ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Targeted Therapy ◽  
Cancer Cells ◽  
Breast Cancer Cells ◽  
Silicon Nanoparticles ◽  
Targeted Imaging ◽  
Imprinted Polymer ◽  
Fluorescence Diagnosis ◽  
Specific Fluorescence ◽  
Epitope Approach

Dual-template imprinted polymer coated silicon nanoparticles with epitope imprinting achieved specific fluorescence diagnosis and targeted therapy of breast cancer cells.

Thermo-sensitive imprinted polymer embedded carbon dots using epitope approach

2016 ◽  
Vol 79 ◽  
pp. 187-192 ◽  
Author(s):  
Dong-Yan Li ◽  
Xue-Mei Zhang ◽  
Yun-Jing Yan ◽  
Xi-Wen He ◽  
Wen-You Li ◽  
...  
Keyword(s):  
Carbon Dots ◽  
Imprinted Polymer ◽  
Epitope Approach
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