Structural Superiority of Guanidinium-Rich, Four-Armed Copolypeptides: Role of Multiple Peptide–Membrane Interactions in Enhancing Bacterial Membrane Perturbation and Permeability

2020 ◽  
Vol 12 (16) ◽  
pp. 18363-18374 ◽  
Author(s):  
Jing Wang ◽  
Chao Lu ◽  
Yin Shi ◽  
Xiaoqian Feng ◽  
Biyuan Wu ◽  
...  
Keyword(s):  
Bacterial Membrane ◽  
Membrane Interactions ◽  
Membrane Perturbation

scholarly journals Isosteric substitution in cationic-amphiphilic polymers reveals an important role for hydrogen bonding in bacterial membrane interactions

2016 ◽  
Vol 7 (7) ◽  
pp. 4613-4623 ◽  
Author(s):  
D. S. S. M. Uppu ◽  
M. M. Konai ◽  
U. Baul ◽  
P. Singh ◽  
T. K. Siersma ◽  
...  
Keyword(s):  
Hydrogen Bonding ◽  
Amphiphilic Polymers ◽  
Bacterial Membrane ◽  
Membrane Interactions ◽  
Bacterial Membranes

The important role of hydrogen bonding in the interactions of cationic-amphiphilic polymers with bacterial membranes has been reported.

scholarly journals Towards designing globular antimicrobial peptide mimics: role of polar functional groups in biomimetic ternary antimicrobial polymers

Soft Matter ◽  
2021 ◽  
Author(s):  
Garima Rani ◽  
Kenichi Kuroda ◽  
Satyavani Vemparala
Keyword(s):  
Molecular Dynamics ◽  
Antimicrobial Peptide ◽  
Bacterial Membrane ◽  
Simulation Data ◽  
Antimicrobial Polymers ◽  
Polar Groups ◽  
Methacrylate Polymers ◽  
Dynamics Simulations ◽  
Polar Functional Groups

Using atomistic molecular dynamics simulations, we study the interaction of ternary methacrylate polymers, composed of charged cationic, hydrophobic and neutral polar groups, with model bacterial membrane. Our simulation data shows...

scholarly journals Metagenomic Profiling of Fecal-Derived Bacterial Membrane Vesicles in Crohn’s Disease Patients

Cells ◽  
2021 ◽  
Vol 10 (10) ◽  
pp. 2795
Author(s):  
Nader Kameli ◽  
Heike E. F. Becker ◽  
Tessa Welbers ◽  
Daisy M. A. E. Jonkers ◽  
John Penders ◽  
...  
Keyword(s):  
Crohn’S Disease ◽  
Crohn's Disease ◽  
Membrane Vesicles ◽  
Amplicon Sequencing ◽  
Size Exclusion ◽  
Bacterial Membrane ◽  
Microbial Composition ◽  
Fecal Samples ◽  
Exclusion Chromatography

Background: In the past, many studies suggested a crucial role for dysbiosis of the gut microbiota in the etiology of Crohn’s disease (CD). However, despite being important players in host–bacteria interaction, the role of bacterial membrane vesicles (MV) has been largely overlooked in the pathogenesis of CD. In this study, we addressed the composition of the bacterial and MV composition in fecal samples of CD patients and compared this to the composition in healthy individuals. Methods: Fecal samples from six healthy subjects (HC) in addition to twelve CD patients (six active, six remission) were analyzed in this study. Fecal bacterial membrane vesicles (fMVs) were isolated by a combination of ultrafiltration and size exclusion chromatography. DNA was obtained from the fMV fraction, the pellet of dissolved feces as bacterial DNA (bDNA), or directly from feces as fecal DNA (fDNA). The fMVs were characterized by nanoparticle tracking analysis and cryo-electron microscopy. Amplicon sequencing of 16s rRNA V4 hypervariable gene regions was conducted to assess microbial composition of all fractions. Results: Beta-diversity analysis showed that the microbial community structure of the fMVs was significantly different from the microbial profiles of the fDNA and bDNA. However, no differences were observed in microbial composition between fDNA and bDNA. The microbial richness of fMVs was significantly decreased in CD patients compared to HC, and even lower in active patients. Profiling of fDNA and bDNA demonstrated that Firmicutes was the most dominant phylum in these fractions, while in fMVs Bacteroidetes was dominant. In fMV, several families and genera belonging to Firmicutes and Proteobacteria were significantly altered in CD patients when compared to HC. Conclusion: The microbial alterations of MVs in CD patients particularly in Firmicutes and Proteobacteria suggest a possible role of MVs in host-microbe symbiosis and induction or progression of inflammation in CD pathogenesis. Yet, the exact role for these fMV in the pathogenesis of the disease needs to be elucidated in future studies.

scholarly journals Role of PIP2-Dependent Membrane Interactions in Vinculin Activation, Motility and Force Transmission

2017 ◽  
Vol 112 (3) ◽  
pp. 479a
Author(s):  
Sharon L. Campbell ◽  
Peter M. Thompson ◽  
Srinivas Ramachandran ◽  
Lindsay Case ◽  
Nikolay Dokholyan ◽  
...  
Keyword(s):  
Force Transmission ◽  
Membrane Interactions

Role of palmitate and myristate in G protein–membrane interactions

2007 ◽  
Vol 149 ◽  
pp. S23
Author(s):  
Rafael Alvarez ◽  
Jesús Casas ◽  
Silvia Terés ◽  
Victoria Llado ◽  
Pablo V. Escribá
Keyword(s):  
G Protein ◽  
Membrane Interactions ◽  
Protein Membrane

scholarly journals Hexapeptide Derivatives of Glycopeptide Antibiotics: Tools for Mechanism of Action Studies

2002 ◽  
Vol 46 (8) ◽  
pp. 2344-2348 ◽  
Author(s):  
Norris E. Allen ◽  
Deborah L. LeTourneau ◽  
Joe N. Hobbs ◽  
Richard C. Thompson
Keyword(s):  
Micrococcus Luteus ◽  
Membrane Vesicles ◽  
Antibacterial Activities ◽  
Mechanisms Of Action ◽  
Side Chain ◽  
Bacterial Membrane ◽  
Glycopeptide Antibiotics ◽  
Bacterial Membranes ◽  
Derivatives Of

ABSTRACT Hexapeptide (des-N-methylleucyl) derivatives of LY264826 were prepared in order to examine further the role of N-substituted hydrophobic side chains in defining the mechanisms of action of semisynthetic glycopeptide antibiotics. The hexapeptide of LY264826 binds to the cell wall intermediate analog l-Lys-d-Ala-d-Ala with a 100-fold lower affinity than LY264826 and inhibits Micrococcus luteus almost 200-fold more poorly than LY264826 does. Alkylation of the 4-epi-vancosamine moiety of the disaccharide significantly enhanced the antibacterial activity of the hexapeptide. Alkylation did not affect the binding affinity for d-alanyl-d-alanine residues; however, it did enhance dimerization 7,000-fold and enhanced binding to bacterial membrane vesicles noticeably compared with the levels of dimerization and binding for the unsubstituted hexapeptide. The findings from this study complement those presented in an earlier report (N. E. Allen, D. L. LeTourneau, and J. N. Hobbs, Jr., J. Antibiot. 50:677-684, 1997) and are consistent with the conclusion that the enhanced antibacterial activities of semisynthetic glycopeptide antibiotics derive from the ability of the hydrophobic side chain to markedly affect both dimerization and binding to bacterial membranes.

Influence of lipid composition of model membranes on methacrylate antimicrobial polymer–membrane interactions

Soft Matter ◽  
2017 ◽  
Vol 13 (41) ◽  
pp. 7665-7676 ◽  
Author(s):  
Upayan Baul ◽  
Satyavani Vemparala
Keyword(s):  
Molecular Dynamics ◽  
Molecular Dynamics Simulations ◽  
Lipid Composition ◽  
Polymer Membrane ◽  
Model Membranes ◽  
Membrane Interactions ◽  
Dynamics Simulations

Using atomistic molecular dynamics simulations, the role of lipid composition in the interactions of multiple methacrylate antimicrobial polymer agents with model membranes, and the consequent response of the membranes is studied.

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