Photo-Electro Active Nanocomposite Silk Hydrogel for Spatiotemporal Controlled Release of Chemotherapeutics: An In Vivo Approach toward Suppressing Solid Tumor Growth

2020 ◽  
Vol 12 (25) ◽  
pp. 27905-27916 ◽  
Author(s):  
Ankit Gangrade ◽  
Basveshwar Gawali ◽  
Praveen Kumar Jadi ◽  
Vegi G. M. Naidu ◽  
Biman B. Mandal
Keyword(s):  
Controlled Release ◽  
Tumor Growth ◽  
Solid Tumor ◽  
Solid Tumor Growth ◽  
Silk Hydrogel

Inhibiting Solid Tumor Growth In Vivo by Non-Tumor-Penetrating Nanomedicine

Small ◽  
2017 ◽  
Vol 13 (12) ◽  
pp. 1600954 ◽  
Author(s):  
Shixian Lv ◽  
Zhaohui Tang ◽  
Wantong Song ◽  
Dawei Zhang ◽  
Mingqiang Li ◽  
...  
Keyword(s):  
Tumor Growth ◽  
Solid Tumor ◽  
Solid Tumor Growth

scholarly journals Platelet microRNAs inhibit primary tumor growth via broad modulation of tumor cell mRNA expression in ectopic pancreatic cancer in mice

PLoS ONE ◽  
2021 ◽  
Vol 16 (12) ◽  
pp. e0261633
Author(s):  
Jeremy G. T. Wurtzel ◽  
Sophia Lazar ◽  
Sonali Sikder ◽  
Kathy Q. Cai ◽  
Igor Astsaturov ◽  
...  
Keyword(s):  
Gene Expression ◽  
Tumor Growth ◽  
Tumor Cell ◽  
Tumor Cells ◽  
Solid Tumor ◽  
Primary Tumor ◽  
Primary Tumor Growth ◽  
Primary Tumors ◽  
Solid Tumor Growth

We investigated the contributions of platelet microRNAs (miRNAs) to the rate of growth and regulation of gene expression in primary ectopic tumors using mouse models. We previously identified an inhibitory role for platelets in solid tumor growth, mediated by tumor infiltration of platelet microvesicles (microparticles) which are enriched in platelet-derived miRNAs. To investigate the specific roles of platelet miRNAs in tumor growth models, we implanted pancreatic ductal adenocarcinoma cells as a bolus into mice with megakaryocyte-/platelet-specific depletion of mature miRNAs. We observed an ~50% increase in the rate of growth of ectopic primary tumors in these mice compared to controls including at early stages, associated with reduced apoptosis in the tumors, in particular in tumor cells associated with platelet microvesicles—which were depleted of platelet-enriched miRNAs—demonstrating a specific role for platelet miRNAs in modulation of primary tumor growth. Differential expression RNA sequencing of tumor cells isolated from advanced primary tumors revealed a broad cohort of mRNAs modulated in the tumor cells as a function of host platelet miRNAs. Altered genes comprised 548 up-regulated transcripts and 43 down-regulated transcripts, mostly mRNAs altogether spanning a variety of growth signaling pathways–notably pathways related to epithelial-mesenchymal transition—in tumor cells from platelet miRNA-deleted mice compared with those from control mice. Tumors in platelet miRNA-depleted mice showed more sarcomatoid growth and more advanced tumor grade, indicating roles for host platelet miRNAs in tumor plasticity. We further validated increased protein expression of selected genes associated with increased cognate mRNAs in the tumors due to platelet miRNA depletion in the host animals, providing proof of principle of widespread effects of platelet miRNAs on tumor cell functional gene expression in primary tumors in vivo. Together, these data demonstrate that platelet-derived miRNAs modulate solid tumor growth in vivo by broad-spectrum restructuring of the tumor cell transcriptome.

scholarly journals Platelet microparticles infiltrating solid tumors transfer miRNAs that suppress tumor growth

Blood ◽  
2017 ◽  
Vol 130 (5) ◽  
pp. 567-580 ◽  
Author(s):  
James V. Michael ◽  
Jeremy G. T. Wurtzel ◽  
Guang Fen Mao ◽  
A. Koneti Rao ◽  
Mikhail A. Kolpakov ◽  
...  
Keyword(s):  
Tumor Growth ◽  
Tumor Cell ◽  
Solid Tumors ◽  
Tumor Cells ◽  
Solid Tumor ◽  
Solid Tumor Growth ◽  
Key Points ◽  
Platelet Microparticles

Key Points Platelet MPs infiltrate solid tumors and transfer platelet-derived miRNAs to tumor cells within solid tumors in vivo. Transfer of platelet miRNAs to tumor cells results in downregulation of tumor cell genes and inhibition of solid tumor growth.

Sign in / Sign up

Export Citation Format

Share Document