scholarly journals Platinum/Graphene Oxide Coated Microfabricated Arrays for Multinucleus Neural Activities Detection in the Rat Models of Parkinson’s Disease Treated by Apomorphine

2019 ◽  
Vol 2 (9) ◽  
pp. 4010-4019 ◽  
Author(s):  
Guihua Xiao ◽  
Yilin Song ◽  
Yu Zhang ◽  
Shengwei Xu ◽  
Yu Xing ◽  
...  
Keyword(s):  
Parkinson’S Disease ◽  
Parkinson's Disease ◽  
Graphene Oxide ◽  
Rat Models ◽  
Neural Activities ◽  
Microfabricated Arrays

Gait analysis in three different 6-hydroxydopamine rat models of Parkinson's disease

2015 ◽  
Vol 584 ◽  
pp. 184-189 ◽  
Author(s):  
Ming Zhou ◽  
Wangming Zhang ◽  
Jingyu Chang ◽  
Jun Wang ◽  
Weixin Zheng ◽  
...  
Keyword(s):  
Parkinson’S Disease ◽  
Parkinson's Disease ◽  
Gait Analysis ◽  
Rat Models ◽  
6 Hydroxydopamine

scholarly journals Neuroprotective effects of 5-aminolevulinic acid against neurodegeneration in rat models of Parkinson's disease and stroke

2020 ◽  
Vol 144 (3) ◽  
pp. 183-187
Author(s):  
Masanori Hijioka ◽  
Kanori Kitamura ◽  
Daijiro Yanagisawa ◽  
Kaneyasu Nishimura ◽  
Kazuyuki Takata ◽  
...  
Keyword(s):  
Parkinson’S Disease ◽  
Parkinson's Disease ◽  
Aminolevulinic Acid ◽  
Neuroprotective Effects ◽  
Rat Models

Interaction of 5-S-cysteinyl-dopamine with graphene oxide: an experimental and theoretical study for the detection of a Parkinson's disease biomarker

2019 ◽  
Vol 43 (40) ◽  
pp. 15861-15870 ◽  
Author(s):  
Isidro Badillo-Ramírez ◽  
Bruno Landeros-Rivera ◽  
Emmanuel de la O-Cuevas ◽  
Rubicelia Vargas ◽  
Jorge Garza ◽  
...  
Keyword(s):  
Parkinson’S Disease ◽  
Raman Spectroscopy ◽  
Parkinson's Disease ◽  
Graphene Oxide ◽  
Theoretical Analysis ◽  
Theoretical Study ◽  
Disease Biomarker

Spectroscopic and theoretical analysis in the adsorption of 5-S-Cys-DA over GO for the development of platform biosensors with Raman spectroscopy.

scholarly journals Graphene Oxide and Reduced Derivatives, as Powder or Film Scaffolds, Differentially Promote Dopaminergic Neuron Differentiation and Survival

2020 ◽  
Vol 14 ◽  
Author(s):  
Noela Rodriguez-Losada ◽  
Rune Wendelbob ◽  
M. Carmen Ocaña ◽  
Amelia Diaz Casares ◽  
Roberto Guzman de Villoría ◽  
...  
Keyword(s):  
Parkinson’S Disease ◽  
Parkinson's Disease ◽  
Graphene Oxide ◽  
Carbon Nanomaterials ◽  
Cell Loss ◽  
Synaptic Proteins ◽  
New Drugs ◽  
Neural Regeneration ◽  
Protein Levels ◽  
And Function

Emerging scaffold structures made of carbon nanomaterials, such as graphene oxide (GO) have shown efficient bioconjugation with common biomolecules. Previous studies described that GO promotes the differentiation of neural stem cells and may be useful for neural regeneration. In this study, we examined the capacity of GO, full reduced (FRGO), and partially reduced (PRGO) powder and film to support survival, proliferation, differentiation, maturation, and bioenergetic function of a dopaminergic (DA) cell line derived from the mouse substantia nigra (SN4741). Our results show that the morphology of the film and the species of graphene (GO, PRGO, or FRGO) influences the behavior and function of these neurons. In general, we found better biocompatibility of the film species than that of the powder. Analysis of cell viability and cytotoxicity showed good cell survival, a lack of cell death in all GO forms and its derivatives, a decreased proliferation, and increased differentiation over time. Neuronal maturation of SN4741 in all GO forms, and its derivatives were assessed by increased protein levels of tyrosine hydroxylase (TH), dopamine transporter (DAT), the glutamate inward rectifying potassium channel 2 (GIRK2), and of synaptic proteins, such as synaptobrevin and synaptophysin. Notably, PRGO-film increased the levels of Tuj1 and the expression of transcription factors specific for midbrain DA neurons, such as Pitx3, Lmx1a, and Lmx1b. Bioenergetics and mitochondrial dysfunction were evaluated by measuring oxygen consumption modified by distinct GO species and were different between powder and film for the same GO species. Our results indicate that PRGO-film was the best GO species at maintaining mitochondrial function compared to control. Finally, different GO forms, and particularly PRGO-film was also found to prevent the loss of DA cells and the decrease of the α-synuclein (α-syn) in a molecular environment where oxidative stress has been induced to model Parkinson's disease. In conclusion, PRGO-film is the most efficient graphene species at promoting DA differentiation and preventing DA cell loss, thus becoming a suitable scaffold to test new drugs or develop constructs for Parkinson's disease cell replacement therapy.

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