scholarly journals Single or Mixed Tethered Peptides To Promote hMSC Differentiation toward Osteoblastic Lineage

2018 ◽  
Vol 1 (6) ◽  
pp. 1800-1809 ◽  
Author(s):  
Laurence Padiolleau ◽  
Christel Chanseau ◽  
Stéphanie Durrieu ◽  
Pascale Chevallier ◽  
Gaétan Laroche ◽  
...  
Keyword(s):  
Osteoblastic Lineage

Increased formation and decreased resorption of bone in mice with elevated vitamin D receptor in mature cells of the osteoblastic lineage

2000 ◽  
Vol 14 (13) ◽  
pp. 1908-1916 ◽  
Author(s):  
Edith M. Gardiner ◽  
Paul A. Baldock ◽  
Gethin P. Thomas ◽  
Natalie A. Sims ◽  
N. Kathryn Henderson ◽  
...  
Keyword(s):  
Vitamin D ◽  
Vitamin D Receptor ◽  
Osteoblastic Lineage

scholarly journals High Throughput Transcriptome Profiling of Lithium Stimulated Human Mesenchymal Stem Cells Reveals Priming towards Osteoblastic Lineage

PLoS ONE ◽  
2013 ◽  
Vol 8 (1) ◽  
pp. e55769 ◽  
Author(s):  
Neeraj Kumar Satija ◽  
Deepa Sharma ◽  
Farhat Afrin ◽  
Rajendra P. Tripathi ◽  
Gurudutta Gangenahalli
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
High Throughput ◽  
Human Mesenchymal Stem Cells ◽  
Transcriptome Profiling ◽  
Osteoblastic Lineage

scholarly journals Peri-prosthetic tissue cells show osteogenic capacity to differentiate into the osteoblastic lineage

2017 ◽  
Vol 35 (8) ◽  
pp. 1732-1742 ◽  
Author(s):  
Monique A.E. Schoeman ◽  
Angela E. Oostlander ◽  
Karien Ede Rooij ◽  
Edward R. Valstar ◽  
Rob G.H.H. Nelissen
Keyword(s):  
Osteoblastic Lineage ◽  
Tissue Cells

scholarly journals High Bone Resorption in Adult Aging Transgenic Mice Overexpressing Cbfa1/Runx2 in Cells of the Osteoblastic Lineage

2002 ◽  
Vol 22 (17) ◽  
pp. 6222-6233 ◽  
Author(s):  
Valérie Geoffroy ◽  
Michaela Kneissel ◽  
Brigitte Fournier ◽  
Alan Boyde ◽  
Patrick Matthias
Keyword(s):  
Bone Marrow ◽  
Bone Resorption ◽  
Bone Formation ◽  
Transgenic Mice ◽  
Wild Type ◽  
Osteoblastic Lineage ◽  
High Bone ◽  
Transgenic Cells ◽  
Primary Osteoblasts ◽  
In Cells

ABSTRACT The runt family transcription factor core-binding factor α1 (Cbfa1) is essential for bone formation during development. Surprisingly, transgenic mice overexpressing Cbfa1 under the control of the 2.3-kb collagen type I promoter developed severe osteopenia that increased progressively with age and presented multiple fractures. Analysis of skeletally mature transgenic mice showed that osteoblast maturation was affected and that specifically in cortical bone, bone resorption as well as bone formation was increased, inducing high bone turnover rates and a decreased degree of mineralization. To understand the origin of the increased bone resorption, we developed bone marrow stromal cell cultures and reciprocal coculture of primary osteoblasts and spleen cells from wild-type or transgenic mice. We showed that transgenic cells of the osteoblastic lineage induced an increased number of tartrate-resistant acid phosphatase-positive multinucleated cells, suggesting that primary osteoblasts as well as bone marrow stromal cells from transgenic mice have stronger osteoclastogenic properties than cells derived from wild-type animals. We investigated the candidate genes whose altered expression could trigger this increase in bone resorption, and we found that the expression of receptor activator of NF-κB ligand (RANKL) and collagenase 3, two factors involved in bone formation-resorption coupling, was markedly increased in transgenic cells. Our data thus suggest that overexpression of Cbfa1 in cells of the osteoblastic lineage does not necessarily induce a substantial increase in bone formation in the adult skeleton but has a positive effect on osteoclast differentiation in vitro and can also dramatically enhance bone resorption in vivo, possibly through increased RANKL expression.

scholarly journals The Notch Ligand Jagged1 Regulates the Osteoblastic Lineage by Maintaining the Osteoprogenitor Pool

2017 ◽  
Vol 32 (6) ◽  
pp. 1320-1331 ◽  
Author(s):  
Rialnat A Lawal ◽  
Xichao Zhou ◽  
Kaylind Batey ◽  
Corey M Hoffman ◽  
Mary A Georger ◽  
...  
Keyword(s):  
Notch Ligand ◽  
Osteoblastic Lineage

Bioinspired Quercitrin Nanocoatings: A Fluorescence-Based Method for Their Surface Quantification, and Their Effect on Stem Cell Adhesion and Differentiation to the Osteoblastic Lineage

2015 ◽  
Vol 7 (30) ◽  
pp. 16857-16864 ◽  
Author(s):  
Alba Córdoba ◽  
Marta Monjo ◽  
Margarita Hierro-Oliva ◽  
María Luisa González-Martín ◽  
Joana Maria Ramis
Keyword(s):  
Stem Cell ◽  
Cell Adhesion ◽  
Osteoblastic Lineage

Identification and Characterization of Osteoblastic Niche Cells That Regulate Hematopoietic Stem Cells.

Blood ◽  
2007 ◽  
Vol 110 (11) ◽  
pp. 1412-1412
Author(s):  
Yuka Nakamura ◽  
Fumio Arai ◽  
Yumiko Gomei ◽  
Kentarou Hosokawa ◽  
Hiroki Yoshihara ◽  
...  
Keyword(s):  
Stem Cells ◽  
Hematopoietic Stem Cells ◽  
Expression Profiles ◽  
Osteoblastic Cells ◽  
Calcium Deposition ◽  
Pcr Analysis ◽  
Isolated Cells ◽  
Differentiation Potential ◽  
Hematopoietic Stem ◽  
Osteoblastic Lineage

Abstract Currently, the niche for long-term hematopoietic stem cells (HSCs) is thought to consist conceptually of two parts: the endosteal surface (the osteoblastic niche) and a sinusoidal endothelium (the vascular niche), and a subset of osteoblasts functions as a key component of the hematopoietic stem cell niche. However, it is still unclear that the precise cellular and molecular contribution of osteoblastic cells on the HSC supportive microenvironment. In this study, we try to characterize the osteoblastic cells and investigate the property of osteoblastic niche cells. For isolation of osteoblastic cells, we treated the bone fragments of femur and tibiae with collagenase following flush-out of the bone marrow (BM). Non-hematopoietic and non-endothelial cells were then enriched by magnetic cell sorting of the CD45-CD31-Ter119- population, and expression of Sca-1 and platelet derived growth factor receptor α (PDGFRα) was analyzed. FACS analysis showed that CD45-CD31-TER119- cells were subdivided into three fractions: Sca-1+PDGFRα+, Sca-1-PDGFRα-, Sca-1-PDGFRα+. First we examined the multilineage differentiation potential of three populations. Although Sca-1- fractions efficiently differentiated into the osteoblastic lineage and showed calcium deposition, these cells hardly differentiated into adipocytes. In contrast to the Sca-1- cells, we found that Sca-1+PDGFRα+ cells can differentiate into osteoblastic and adipocytic lineages, suggesting that Sca-1+ cells have multi-potency. Next we examined the expression of osteoblastic marker expression by quantitative RT-PCR analysis, and found that Sca-1- populations expressed Runx2 and OB-cadherin. Alkaline phosphatase (ALP) staining of freshly isolated cells showed that Sca-1- fractions expressed ALP, while Sca-1+ cells did not express ALP. These data suggest that Sca-1- populations were the cell fractions, which were already committed to osteoblastic lineage. In addition, osteocalcin was expressed in PDGFRα+ fraction in Sca-1- cells, indicating that Sca-1-PDGFRα+ cells are more mature osteoblastic cells than Sca-1-PDGFRα-cells. Furthermore, N-cadherin was specifically expressed in Sca-1-PDGFRα+ cells, suggesting that N-cadherin was up-regulated with the maturation of osteoblastic cells. In addition, N-cadherin expression was up-regulated in Sca-1-PDGFRα+ cells with the postnatal development of BM. Interestingly, in the freshly isolated cells, we found that Sca-1+PDGFRα+ cells showed higher expression of Angiopoietin-1 (Ang-1), compared to Sca-1- fractions. Ang-1 expression was up-regulated in Sca-1-PDGFRα+ cells after over night incubation. Next we investigated the ability of these fractionated cells to support hematopoiesis. We examined the capacity of these fractionated cells on maintenance of colony formation ability of BM linage-Sca-1+c-Kit+ cells after 5 days of co-culture. Although CFU-C formation was supported Sca-1+PDGFRα+ cells, Sca-1-PDGFRα+ cells maintained CFU-Mix formation compared to the Sca-1+PDGFRα+ and Sca-1-PDGFRα-cells. From these data above, we hypothesize that multiple osteoblastic populations form a “niche complex” and collaborate with other supporting cells, such as CXCL12-abundant reticular (CAR) cells, to support HSCs, and that N-cadherin+ osteoblastic cells provide a foothold for anchoring of quiescent HSCs. Now we are investigating the gene expression profiles of these three populations and are tying to clarify the changes of characteristics of osteoblastic cells during postnatal BM development.

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