Size Dependency of Selective Cellular Uptake of Epigallocatechin Gallate-modified Gold Nanoparticles for Effective Radiosensitization

2021 ◽  
Author(s):  
Ning Gan ◽  
Chihiro Wakayama ◽  
Sachiko Inubushi ◽  
Tomonari Kunihisa ◽  
Sachiko Mizumoto ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Cellular Uptake ◽  
Epigallocatechin Gallate ◽  
Size Dependency

scholarly journals Epigallocatechin Gallate-Gold Nanoparticles Exhibit Superior Antitumor Activity Compared to Conventional Gold Nanoparticles: Potential Synergistic Interactions

Nanomaterials ◽  
2019 ◽  
Vol 9 (3) ◽  
pp. 396 ◽  
Author(s):  
Suhash Chavva ◽  
Sachin Deshmukh ◽  
Rajashekhar Kanchanapally ◽  
Nikhil Tyagi ◽  
Jason Coym ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Antitumor Activity ◽  
Cancer Cells ◽  
Cellular Uptake ◽  
Nuclear Translocation ◽  
Epigallocatechin Gallate ◽  
Drug Carriers ◽  
Water Soluble ◽  
Tetrazolium Salt ◽  
Uptake Studies

Epigallocatechin gallate (EGCG) possesses significant antitumor activity and binds to laminin receptors, overexpressed on cancer cells, with high affinity. Gold nanoparticles (GNPs) serve as excellent drug carriers and protect the conjugated drug from enzymatic metabolization. Citrate-gold nanoparticles (C-GNPs) and EGCG-gold nanoparticles (E-GNPs) were synthesized by reduction methods and characterized with UV-visible spectroscopy, transmission electron microscopy (TEM), and dynamic light scattering (DLS). Cytotoxicity of citrate, EGCG, C-GNPs, and E-GNPs was evaluated by the water-soluble tetrazolium salt (WST-1) assay. Nanoparticle cellular uptake studies were performed by TEM and atomic absorption spectroscopy (AAS). Dialysis method was employed to assess drug release. Cell viability studies showed greater growth inhibition by E-GNPs compared to EGCG or C-GNPs. Cellular uptake studies revealed that, unlike C-GNPs, E-GNPs were taken up more efficiently by cancerous cells than noncancerous cells. We found that E-GNP nanoformulation releases EGCG in a sustained fashion. Furthermore, data showed that E-GNPs induced more apoptosis in cancer cells compared to EGCG and C-GNPs. From the mechanistic standpoint, we observed that E-GNPs inhibited the nuclear translocation and transcriptional activity of nuclear factor-kappaB (NF-κB) with greater potency than EGCG, whereas C-GNPs were only minimally effective. Altogether, our data suggest that E-GNPs can serve as potent tumor-selective chemotoxic agents.

Tailoring Cellular Uptake of Gold Nanoparticles Via the Hydrophilic-to-Hydrophobic Ratio of their (Co)polymer Coating

2015 ◽  
Vol 25 (22) ◽  
pp. 3433-3439 ◽  
Author(s):  
Zhiyue Zhang ◽  
Katleen Van Steendam ◽  
Samarendra Maji ◽  
Lieve Balcaen ◽  
Yulia Anoshkina ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Cellular Uptake ◽  
Polymer Coating

Abstract 394: ApoE3-containing High Density Lipoprotein Serves as a Multifunctional Platform for Delivery of Gold Nanoparticles for Hyperthermic Treatment

2016 ◽  
Vol 36 (suppl_1) ◽  
Author(s):  
Vasanthy Narayanaswami ◽  
Skylar T Chuang ◽  
Young-Seok Shon
Keyword(s):  
Gold Nanoparticles ◽  
Cellular Uptake ◽  
Effective Means ◽  
Density Lipoprotein ◽  
Aqueous Solubility ◽  
Soluble Form ◽  
Glioblastoma Cells ◽  
Plasmon Band ◽  
Cytotoxicity Studies ◽  
Multifunctional Platform

We have developed a novel HDL-based multifunctional platform for transport and delivery of highly hydrophobic gold nanoparticles (AuNP) bearing photothermic properties. We exploit the ability of apolipoprotein E3 (apoE3) to act as a high affinity ligand for the low-density lipoprotein receptor (LDLr) to gain entry into glioblastoma cells. The issue of poor aqueous solubility of AuNP was overcome by integrating them with phospholipids and apoE3, yielding reconstituted rHDL bearing 3, 10, or 10 nm AuNP. UV-Vis spectra of rHDL-AuNP indicated the presence of stable particles with surface plasmon band at ~530 nm, a signature feature of AuNP. Transmission electron microscopy (TEM) revealed discoidal geometry for rHDL with 3, 10 and 17 nm particles. Co-immunoprecipitation assay using a soluble form of the LDLr indicated robust binding of rHDL-AuNP to the receptor. Immunofluorescence analysis indicated that all 3 rHDL particles were internalized by glioblastoma cells, as revealed by the presence of punctate, peri-nuclear endocytic/lysosomal vesicles; this suggests cellular uptake of rHDL-AuNP by receptor-mediated endocytosis. Cellular uptake was further confirmed by TEM, in which aggregated AuNP were found in the endosomal-lysosomal compartments. Finally, cytotoxicity studies demonstrated that more than 50% of cells were viable with rHDL-AuNP treatment of up to 0.1 mg/ml for 24 h. The findings that apoE3: (i) acts as a detergent in solubilizing and dramatically improving the aqueous solubility of AuNP, and, (ii) facilitates cellular uptake of rHDL-AuNP by the LDLr pathway, are significant since they offer an effective means of delivering AuNP across the cell membrane. This is particularly relevant in tumor cells since they overexpress LDLr to meet the high demand for cholesterol that is required for rapid proliferation and membrane biogenesis.

Increased cellular uptake of peptide-modified PEGylated gold nanoparticles

2017 ◽  
Vol 494 (1-2) ◽  
pp. 339-345 ◽  
Author(s):  
Bo He ◽  
Dan Yang ◽  
Mengmeng Qin ◽  
Yuan Zhang ◽  
Bing He ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Cellular Uptake

scholarly journals Targeting dendritic cells through gold nanoparticles: A review on the cellular uptake and subsequent immunological properties

2017 ◽  
Vol 91 ◽  
pp. 123-133 ◽  
Author(s):  
Suhana Ahmad ◽  
Anes Ateqah Zamry ◽  
Hern-Tze Tina Tan ◽  
Kah Keng Wong ◽  
JitKang Lim ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Dendritic Cells ◽  
Cellular Uptake ◽  
Immunological Properties

Analytical methodology for studying cellular uptake, processing and localization of gold nanoparticles

2019 ◽  
Vol 1052 ◽  
pp. 1-9 ◽  
Author(s):  
Magdalena Matczuk ◽  
Lena Ruzik ◽  
Svetlana S. Aleksenko ◽  
Bernhard K. Keppler ◽  
Maciej Jarosz ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Cellular Uptake ◽  
Analytical Methodology

Morphological Diversity, Protein Adsorption, and Cellular Uptake of Polydopamine-Coated Gold Nanoparticles

Langmuir ◽  
2018 ◽  
Vol 34 (46) ◽  
pp. 14033-14045 ◽  
Author(s):  
Kwun Hei Samuel Sy ◽  
Lok Wai Cola Ho ◽  
Wilson Chun Yu Lau ◽  
Ho Ko ◽  
Chung Hang Jonathan Choi
Keyword(s):  
Gold Nanoparticles ◽  
Protein Adsorption ◽  
Cellular Uptake ◽  
Morphological Diversity

Cellular uptake and cytotoxicity of chitosan–caseinophosphopeptides nanocomplexes loaded with epigallocatechin gallate

2012 ◽  
Vol 89 (2) ◽  
pp. 362-370 ◽  
Author(s):  
Bing Hu ◽  
Yuwen Ting ◽  
Xiaoxiong Zeng ◽  
Qingrong Huang
Keyword(s):  
Cellular Uptake ◽  
Epigallocatechin Gallate
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