Dual-Emission Carbonized Polymer Dots for Ratiometric pH Sensing, pH-Dependent Generation of Singlet Oxygen, and Imaging-Guided Dynamics Monitoring of Photodynamic Therapy

2021 ◽  
Author(s):  
Xiaoxiao Hu ◽  
Qiang Zhang ◽  
Xiaomei Dai ◽  
Junyong Sun ◽  
Feng Gao
Keyword(s):  
Photodynamic Therapy ◽  
Singlet Oxygen ◽  
Ph Sensing ◽  
Dual Emission ◽  
Polymer Dots ◽  
Ph Dependent ◽  
Ratiometric Ph Sensing

scholarly journals Ratiometric pH Sensing and Imaging in Living Cells with Dual-Emission Semiconductor Polymer Dots

Molecules ◽  
2019 ◽  
Vol 24 (16) ◽  
pp. 2923
Author(s):  
Piaopiao Chen ◽  
Iqra Ilyas ◽  
Su He ◽  
Yichen Xing ◽  
Zhigang Jin ◽  
...  
Keyword(s):  
Fluorescent Dyes ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Fluorescein Isothiocyanate ◽  
Water Soluble ◽  
Ph Sensing ◽  
Dual Emission ◽  
Polymer Dots ◽  
Ratiometric Ph Sensing

Polymer dots (Pdots) represent newly developed semiconductor polymer nanoparticles and exhibit excellent characteristics as fluorescent probes. To improve the sensitivity and biocompatibility of Pdots ratiometric pH biosensors, we synthesized 3 types of water-soluble Pdots: Pdots-PF, Pdots-PP, and Pdots-PPF by different combinations of fluorescent dyes poly(9,9-dioctylfluorenyl-2,7-diyl) (PFO), poly[(9,9-dioctyl-fluorenyl-2,7-diyl)-co-(1,4-benzo-{2,1′,3}-thiadazole)] (PFBT), and fluorescein isothiocyanate (FITC). We found that Pdots-PPF exhibits optimal performance on pH sensing. PFO and FITC in Pdots-PPF produce pH-insensitive (λ = 439 nm) and pH-sensitive (λ = 517 nm) fluorescence respectively upon a single excitation at 380 nm wavelength, which enables Pdots-PPF ratiometric pH sensing ability. Förster resonance energy transfer (FRET) together with the use of PFBT amplify the FITC signal, which enables Pdots-PPF robust sensitivity to pH. The emission intensity ratio (I517/I439) of Pdots-PPF changes linearly as a function of pH within the range of pH 3.0 to 8.0. Pdots-PPF also possesses desirable reversibility and stability in pH measurement. More importantly, Pdots-PPF was successfully used for cell imaging in Hela cells, exhibiting effective cellular uptake and low cytotoxicity. Our study suggests the promising potential of Pdots-PPF as an in vivo biomarker.

Assessment of the relevance of GaPc substituted with azido-polyethylene glycol chains for photodynamic therapy. Design, synthetic strategy, fluorescence, singlet oxygen generation, and pH-dependent spectroscopic behaviour

2017 ◽  
Vol 41 (18) ◽  
pp. 10027-10036 ◽  
Author(s):  
Veysel Koç ◽  
Sevinc Zehra Topal ◽  
Duygu Aydın Tekdaş ◽  
Özge Dilara Ateş ◽  
Emel Önal ◽  
...  
Keyword(s):  
Photodynamic Therapy ◽  
Polyethylene Glycol ◽  
Singlet Oxygen ◽  
Synthetic Route ◽  
Spectroscopic Behaviour ◽  
Oxygen Generation ◽  
Singlet Oxygen Generation ◽  
Synthetic Strategy ◽  
Ph Dependent ◽  
Therapy Design

New synthetic route and PDT potential of symmetrically and asymmetrically substituted Ga(iii)Pcs with terminal azides.

scholarly journals Development of Ultrabright Semiconducting Polymer Dots for Ratiometric pH Sensing

2011 ◽  
Vol 83 (4) ◽  
pp. 1448-1455 ◽  
Author(s):  
Yang-Hsiang Chan ◽  
Changfeng Wu ◽  
Fangmao Ye ◽  
Yuhui Jin ◽  
Polina B. Smith ◽  
...  
Keyword(s):  
Ph Sensing ◽  
Semiconducting Polymer ◽  
Polymer Dots ◽  
Semiconducting Polymer Dots ◽  
Ratiometric Ph Sensing

Ratiometric pH sensing by FRET-based hybrid semiconducting polymer dots in living cells

2021 ◽  
Author(s):  
Zhaoyang Ding ◽  
Xilin Dou ◽  
Chunfei Wang ◽  
Gang Feng ◽  
Jing Xie ◽  
...  
Keyword(s):  
Living Cells ◽  
Ph Sensing ◽  
Semiconducting Polymer ◽  
Polymer Dots ◽  
Semiconducting Polymer Dots ◽  
Ratiometric Ph Sensing

Optical Properties of Photodynamic Therapy Drugs in Different Environments: The Paradigmatic Case of Temoporfin

2020 ◽  
Author(s):  
busenur Aslanoglu ◽  
Ilya Yakavets ◽  
Vladimir Zorin ◽  
Henri-Pierre Lassalle ◽  
Francesca Ingrosso ◽  
...  
Keyword(s):  
Drug Delivery ◽  
Optical Properties ◽  
Photodynamic Therapy ◽  
Minimally Invasive ◽  
Visible Light ◽  
Cancer Treatment ◽  
Singlet Oxygen ◽  
Tumor Cells ◽  
Molecular Oxygen ◽  
Computational Tools

Computational tools have been used to study the photophysical and photochemical features of photosensitizers in photodynamic therapy (PDT) –a minimally invasive, less aggressive alternative for cancer treatment. PDT is mainly based by the activation of molecular oxygen through the action of a photoexcited sensitizer (photosensitizer). Temoporfin, widely known as mTHPC, is a second-generation photosensitizer, which produces the cytotoxic singlet oxygen when irradiated with visible light and hence destroys tumor cells. However, the bioavailability of the mostly hydrophobic photosensitizer, and hence its incorporation into the cells, is fundamental to achieve the desired effect on malignant tissues by PDT. In this study, we focus on the optical properties of the temoporfin chromophore in different environments –in <i>vacuo</i>, in solution, encapsulated in drug delivery agents, namely cyclodextrin, and interacting with a lipid bilayer.

Design of Novel Photosensitizers and Controlled Singlet Oxygen Generation for Photodynamic Therapy

2021 ◽  
Author(s):  
Esra Tanrıverdi Eçik ◽  
Onur BULUT ◽  
Hasan Hüseyin Kazan ◽  
Elif Şenkuytu ◽  
Bunyemin Cosut
Keyword(s):  
Photodynamic Therapy ◽  
Cancer Treatment ◽  
Singlet Oxygen ◽  
Side Effect ◽  
Side Effect Profile ◽  
High Ability ◽  
Lower Side ◽  
Oxygen Generation ◽  
Singlet Oxygen Generation ◽  
Promising Strategy

Photodynamic therapy (PDT) is a promising strategy in cancer treatment with its relatively lower side effect profile. Undoubtedly, the key component of PDT is the photosensitizers with a high ability...

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