Polyethylene Glycol-Mediated Fusion of Extracellular Vesicles with Cationic Liposomes for the Design of Hybrid Delivery Systems

2021 ◽  
Author(s):  
Dipanjan Mukherjee ◽  
Debashish Paul ◽  
Sushmita Sarker ◽  
Md. Nur Hasan ◽  
Ria Ghosh ◽  
...  
Keyword(s):  
Polyethylene Glycol ◽  
Extracellular Vesicles ◽  
Cationic Liposomes ◽  
Delivery Systems ◽  
Hybrid Delivery

scholarly journals Extracellular Vesicles-Based Drug Delivery Systems: A New Challenge and the Exemplum of Malignant Pleural Mesothelioma

2020 ◽  
Vol 21 (15) ◽  
pp. 5432 ◽  
Author(s):  
Stefano Burgio ◽  
Leila Noori ◽  
Antonella Marino Gammazza ◽  
Claudia Campanella ◽  
Mariantonia Logozzi ◽  
...  
Keyword(s):  
Drug Delivery ◽  
Early Diagnosis ◽  
Extracellular Vesicles ◽  
Delivery System ◽  
Malignant Pleural Mesothelioma ◽  
Cell Communication ◽  
Cell Types ◽  
Delivery Systems ◽  
Pleural Mesothelioma ◽  
Potential Use

Research for the most selective drug delivery to tumors represents a fascinating key target in science. Alongside the artificial delivery systems identified in the last decades (e.g., liposomes), a family of natural extracellular vesicles (EVs) has gained increasing focus for their potential use in delivering anticancer compounds. EVs are released by all cell types to mediate cell-to-cell communication both at the paracrine and the systemic levels, suggesting a role for them as an ideal nano-delivery system. Malignant pleural mesothelioma (MPM) stands out among currently untreatable tumors, also due to the difficulties in achieving an early diagnosis. Thus, early diagnosis and treatment of MPM are both unmet clinical needs. This review looks at indirect and direct evidence that EVs may represent both a new tool for allowing an early diagnosis of MPM and a potential new delivery system for more efficient therapeutic strategies. Since MPM is a relatively rare malignant tumor and preclinical MPM models developed to date are very few and not reliable, this review will report data obtained in other tumor types, suggesting the potential use of EVs in mesothelioma patients as well.

scholarly journals Сationic liposomes as delivery systems for nucleic acids

2020 ◽  
Vol 15 (1) ◽  
pp. 7-27
Author(s):  
A. A. Mikheev ◽  
E. V. Shmendel ◽  
E. S. Zhestovskaya ◽  
G. V. Nazarov ◽  
M. A. Maslov
Keyword(s):  
Gene Therapy ◽  
Nucleic Acids ◽  
Serum Proteins ◽  
Transfection Efficiency ◽  
Biological Fluids ◽  
Genetic Material ◽  
Cationic Liposomes ◽  
Delivery Systems ◽  
Cationic Lipids

Objectives. Gene therapy is based on the introduction of genetic material into cells, tissues, or organs for the treatment of hereditary or acquired diseases. A key factor in the success of gene therapy is the development of delivery systems that can efficiently transfer genetic material to the place of their therapeutic action without causing any associated side effects. Over the past 10 years, significant effort has been directed toward creating more efficient and biocompatible vectors capable of transferring nucleic acids (NAs) into cells without inducing an immune response. Cationic liposomes are among the most versatile tools for delivering NAs into cells; however, the use of liposomes for gene therapy is limited by their low specificity. This is due to the presence of various biological barriers to the complex of liposomes with NA, including instability in biological fluids, interaction with serum proteins, plasma and nuclear membranes, and endosomal degradation. This review summarizes the results of research in recent years on the development of cationic liposomes that are effective in vitro and in vivo. Particular attention is paid to the individual structural elements of cationic liposomes that determine the transfection efficiency and cytotoxicity. The purpose of this review was to provide a theoretical justification of the most promising choice of cationic liposomes for the delivery of NAs into eukaryotic cells and study the effect of the composition of cationic lipids (CLs) on the transfection efficiency in vitro.Results. As a result of the analysis of the related literature, it can be argued that one of the most promising delivery systems of NAs is CL based on cholesterol and spermine with the addition of a helper lipid DOPE. In addition, it was found that varying the composition of cationic liposomes, the ratio of CL to NA, or the size and zeta potential of liposomes has a significant effect on the transfection efficiency.Conclusions. Further studies in this direction should include optimization of the conditions for obtaining cationic liposomes, taking into account the physicochemical properties and established laws. It is necessary to identify mechanisms that increase the efficiency of NA delivery in vitro by searching for optimal structures of cationic liposomes, determining the ratio of lipoplex components, and studying the delivery efficiency and properties of multicomponent liposomes.

scholarly journals Extracellular vesicles nanoarray technology: Immobilization of individual extracellular vesicles on nanopatterned polyethylene glycol-lipid conjugate brushes

PLoS ONE ◽  
2019 ◽  
Vol 14 (10) ◽  
pp. e0224091 ◽  
Author(s):  
Shusuke Yokota ◽  
Hiromi Kuramochi ◽  
Kyohei Okubo ◽  
Akiko Iwaya ◽  
Shoichi Tsuchiya ◽  
...  
Keyword(s):  
Polyethylene Glycol ◽  
Extracellular Vesicles ◽  
Lipid Conjugate

scholarly journals Extracellular Vesicles as Natural Nanosized Delivery Systems for Small-Molecule Drugs and Genetic Material: Steps towards the Future Nanomedicines

2015 ◽  
Vol 18 (3) ◽  
pp. 396 ◽  
Author(s):  
Mustafa Kotmakçı ◽  
Vildan Bozok Çetintaş
Keyword(s):  
Extracellular Vesicles ◽  
Small Molecule ◽  
Protein Delivery ◽  
Drug Loading ◽  
Genetic Material ◽  
Delivery Systems ◽  
Small Molecule Drugs ◽  
The Common ◽  
Therapeutic Protocols ◽  
Targeting Ability

A new platform for drug, gene and peptide-protein delivery is emerging, under the common name of “extracellular vesicles”. Extracellular vesicles (EVs) are 30-1000 nm-sized cell-derived, liposome-like vesicles. Current research on EVs as nano-delivery systems for small-molecule drugs and genetic material, reveal that these tiny, biologically-derived vesicles carry a great potential to boost the efficacy of many therapeutic protocols. Several features of EVs; from efficacy to safety, from passive to active targeting ability, the opportunity to be biologically or chemically labelled, and most importantly, their eobiotic origin make them promising candidate for development of the next generation personalized nanomedicines. The aim of this article is to provide a view on the current research in which EVs are used as drug/genetic material delivery systems. Their application areas, drug loading and targeting strategies, and biodistribution properties are discussed.This article is open to POST-PUBLICATION REVIEW. Registered readers (see “For Readers”) may comment by clicking on ABSTRACT on the issue’s contents page.

Advances in microfluidics for lipid nanoparticles and extracellular vesicles and applications in drug delivery systems

2018 ◽  
Vol 128 ◽  
pp. 84-100 ◽  
Author(s):  
Masatoshi Maeki ◽  
Niko Kimura ◽  
Yusuke Sato ◽  
Hideyoshi Harashima ◽  
Manabu Tokeshi
Keyword(s):  
Drug Delivery ◽  
Extracellular Vesicles ◽  
Drug Delivery Systems ◽  
Lipid Nanoparticles ◽  
Delivery Systems

Gated hybrid delivery systems: En route to sensory materials with inherent signal amplification

2013 ◽  
Vol 257 (17-18) ◽  
pp. 2589-2606 ◽  
Author(s):  
Mandy Hecht ◽  
Estela Climent ◽  
Mustafa Biyikal ◽  
Félix Sancenón ◽  
Ramón Martínez-Máñez ◽  
...  
Keyword(s):  
Signal Amplification ◽  
Delivery Systems ◽  
Hybrid Delivery

Extracellular vesicles as drug delivery systems: Lessons from the liposome field

2014 ◽  
Vol 195 ◽  
pp. 72-85 ◽  
Author(s):  
Roy van der Meel ◽  
Marcel H.A.M. Fens ◽  
Pieter Vader ◽  
Wouter W. van Solinge ◽  
Omolola Eniola-Adefeso ◽  
...  
Keyword(s):  
Drug Delivery ◽  
Extracellular Vesicles ◽  
Drug Delivery Systems ◽  
Delivery Systems
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