scholarly journals Interfacing Live Cells with Surfaces: A Concurrent Control Technique for Quantifying Surface Ligand Activity

2021 ◽  
Author(s):  
Michael C. Robitaille ◽  
Joseph A. Christodoulides ◽  
Patrick J. Calhoun ◽  
Jeff M. Byers ◽  
Marc P. Raphael
Keyword(s):  
Control Technique ◽  
Live Cells ◽  
Concurrent Control ◽  
Surface Ligand ◽  
Ligand Activity

scholarly journals Interfacing Live Cells with Surfaces: A Concurrent Control Technique for Quantifying Surface Ligand Activity

2021 ◽  
Author(s):  
Michael C. Robitaille ◽  
Joseph A. Christodoulides ◽  
Patrick Calhoun ◽  
Jeff M. Byers ◽  
Marc P. Raphael
Keyword(s):  
Cellular Adhesion ◽  
Live Cell ◽  
Control Technique ◽  
Live Cells ◽  
Chip Surface ◽  
Concurrent Control ◽  
Activity Measurements ◽  
Surface Ligand ◽  
Ligand Activity

AbstractSurface ligand activity is a key design parameter for successfully interfacing surfaces with cells - whether in the context of in vitro investigations for understanding cellular signaling pathways or more applied applications in drug delivery and medical implants. Unlike other crucial surface parameters, such as stiffness and roughness, surface ligand activity currently lacks a standardized measurement approach that can be readily paired with live cell investigations. To fill this void, we have developed a concurrent control technique for characterizing in vitro ligand surface activity. Pairs of gold-coated glass chips were biofunctionalized with RGD ligand in a parallel workflow: one chip for in vitro applications and the other for surface plasmon resonance (SPR) based RGD activity characterization. Recombinant αVβ3 integrins were injected over the SPR chip surface as mimics of the cellular membrane bound receptors and the resulting binding kinetics parameterized to quantify ligand activity. These activity measurements were correlated with cell morphological features, measured by interfacing MDA-MB-231 cells with the in vitro chip surfaces on the live cell microscope. We show that the SPR concurrent control approach has multiple advantages based on the facts that SPR is a standardized technique and has the sensitivity to measure ligand activity across the most relevant range of extracellular surface densities. Furthermore, by pairing both SPR and in vitro approaches, a comparison of the results can provide biological insights into the nature of cellular adhesion and dynamics.

Improve the loop frequency of liquid crystal adaptive optics by concurrent control technique

2010 ◽  
Vol 283 (6) ◽  
pp. 946-950 ◽  
Author(s):  
Zhaoliang Cao ◽  
Quanquan Mu ◽  
Lifa Hu ◽  
Yonggang Liu ◽  
Li Xuan
Keyword(s):  
Liquid Crystal ◽  
Adaptive Optics ◽  
Control Technique ◽  
Concurrent Control

4-dimensional, high-resolution imaging of living cells

1992 ◽  
Vol 50 (1) ◽  
pp. 416-417
Author(s):  
Shinya Inoué
Keyword(s):  
Light Microscope ◽  
Living Cells ◽  
Live Cells ◽  
Video Tape ◽  
Active Living ◽  
High Resolution Imaging ◽  
3 Dimensional ◽  
Dynamic Architecture ◽  
Resolution Imaging ◽  
Disk Memory

This paper reports progress of our effort to rapidly capture, and display in time-lapsed mode, the 3-dimensional dynamic architecture of active living cells and developing embryos at the highest resolution of the light microscope. Our approach entails: (A) real-time video tape recording of through-focal, ultrathin optical sections of live cells at the highest resolution of the light microscope; (B) repeat of A at time-lapsed intervals; (C) once each time-lapsed interval, an image at home focus is recorded onto Optical Disk Memory Recorder (OMDR); (D) periods of interest are selected using the OMDR and video tape records; (E) selected stacks of optical sections are converted into plane projections representing different view angles (±4 degrees for stereo view, additional angles when revolving stereos are desired); (F) analysis using A - D.

Multi-mode light microscopy of microtubule assembly dynamics and chromosome movement in vivo and In vitro

1996 ◽  
Vol 54 ◽  
pp. 730-731
Author(s):  
E. D. Salmon ◽  
J. C. Waters ◽  
C. Waterman-Storer
Keyword(s):  
Imaging System ◽  
Ccd Camera ◽  
Transmitted Light ◽  
Microtubule Assembly ◽  
Live Cells ◽  
Optical Efficiency ◽  
Multiple Band ◽  
Multi Mode

We have developed a multi-mode digital imaging system which acquires images with a cooled CCD camera (Figure 1). A multiple band pass dichromatic mirror and robotically controlled filter wheels provide wavelength selection for epi-fluorescence. Shutters select illumination either by epi-fluorescence or by transmitted light for phase contrast or DIC. Many of our experiments involve investigations of spindle assembly dynamics and chromosome movements in live cells or unfixed reconstituted preparations in vitro in which photodamage and phototoxicity are major concerns. As a consequence, a major factor in the design was optical efficiency: achieving the highest image quality with the least number of illumination photons. This principle applies to both epi-fluorescence and transmitted light imaging modes. In living cells and extracts, microtubules are visualized using X-rhodamine labeled tubulin. Photoactivation of C2CF-fluorescein labeled tubulin is used to locally mark microtubules in studies of microtubule dynamics and translocation. Chromosomes are labeled with DAPI or Hoechst DNA intercalating dyes.

Characterization of a Motile Cellular Domain Arising During the Amoebo-Flagellate Transformation of Physarum Polycephalum

1980 ◽  
Vol 38 ◽  
pp. 552-553
Author(s):  
K.I. Pagh ◽  
M.R. Adelman
Keyword(s):  
Cell Shape ◽  
Physarum Polycephalum ◽  
Slime Mold ◽  
Live Cells ◽  
Cellular Domain

Unicellular amoebae of the slime mold Physarum polycephalum undergo marked changes in cell shape and motility during their conversion into flagellate swimming cells (l). To understand the processes underlying motile activities expressed during the amoebo-flagellate transformation, we have undertaken detailed investigations of the organization, formation and functions of subcellular structures or domains of the cell which are hypothesized to play a role in movement. One focus of our studies is on a structure, termed the “ridge” which appears as a flattened extension of the periphery along the length of transforming cells (Fig. 1). Observations of live cells using Nomarski optics reveal two types of movement in this region:propagation of undulations along the length of the ridge and formation and retraction of filopodial projections from its edge. The differing activities appear to be associated with two characteristic morphologies, illustrated in Fig. 1.

Wide-area Power System Oscillation Damping using Model Predictive Control Technique

2011 ◽  
Vol 131 (7) ◽  
pp. 536-541 ◽  
Author(s):  
Tarek Hassan Mohamed ◽  
Abdel-Moamen Mohammed Abdel-Rahim ◽  
Ahmed Abd-Eltawwab Hassan ◽  
Takashi Hiyama
Keyword(s):  
Model Predictive Control ◽  
Power System ◽  
Predictive Control ◽  
Wide Area ◽  
Control Technique ◽  
Oscillation Damping

Parametric Study of Damping Characteristics of Magneto-Rheological Damper: Mathematical and Experimental Approach

2020 ◽  
Vol 15 (3) ◽  
pp. 37-48
Author(s):  
Zubair Rashid Wani ◽  
Manzoor Ahmad Tantray
Keyword(s):  
Structural Control ◽  
Research Work ◽  
Testing Machine ◽  
Input Voltage ◽  
Damping Coefficient ◽  
Control Technique ◽  
Damping Force ◽  
Active Devices ◽  
Active Structural Control ◽  
Magneto Rheological

The present research work is a part of a project was a semi-active structural control technique using magneto-rheological damper has to be performed. Magneto-rheological dampers are an innovative class of semi-active devices that mesh well with the demands and constraints of seismic applications; this includes having very low power requirements and adaptability. A small stroke magneto-rheological damper was mathematically simulated and experimentally tested. The damper was subjected to periodic excitations of different amplitudes and frequencies at varying voltage. The damper was mathematically modeled using parametric Modified Bouc-Wen model of magneto-rheological damper in MATLAB/SIMULINK and the parameters of the model were set as per the prototype available. The variation of mechanical properties of magneto-rheological damper like damping coefficient and damping force with a change in amplitude, frequency and voltage were experimentally verified on INSTRON 8800 testing machine. It was observed that damping force produced by the damper depended on the frequency as well, in addition to the input voltage and amplitude of the excitation. While the damping coefficient (c) is independent of the frequency of excitation it varies with the amplitude of excitation and input voltage. The variation of the damping coefficient with amplitude and input voltage is linear and quadratic respectively. More ever the mathematical model simulated in MATLAB was in agreement with the experimental results obtained.

A Proposal of Cyclic Sleep Control Technique for Backup Resources in ROADM Systems to Reduce Power Consumption of Photonic Network

2014 ◽  
Vol E97.B (12) ◽  
pp. 2698-2705
Author(s):  
Tomoyuki HINO ◽  
Hitoshi TAKESHITA ◽  
Kiyo ISHII ◽  
Junya KURUMIDA ◽  
Shu NAMIKI ◽  
...  
Keyword(s):  
Power Consumption ◽  
Control Technique ◽  
Reduce Power Consumption ◽  
Photonic Network ◽  
Sleep Control

Quasi-Periodic Control Technique for Minimizing Fuel Consumption During Record Vehicle Competition

2013 ◽  
Vol 60 (2) ◽  
pp. 185-197 ◽  
Author(s):  
Paweł Sulikowski ◽  
Ryszard Maronski
Keyword(s):  
Fuel Consumption ◽  
Optimal Control Theory ◽  
Slope Angle ◽  
Control Technique ◽  
Decision Variables ◽  
Aeronautical Engineering ◽  
The One ◽  
Optimal Driving ◽  
The Given ◽  
Engine Power

The problem of the optimal driving technique during the fuel economy competition is reconsidered. The vehicle is regarded as a particle moving on a trace with a variable slope angle. The fuel consumption is minimized as the vehicle covers the given distance in a given time. It is assumed that the run consists of two recurrent phases: acceleration with a full available engine power and coasting down with the engine turned off. The most fuel-efficient technique for shifting gears during acceleration is found. The decision variables are: the vehicle velocities at which the gears should be shifted, on the one hand, and the vehicle velocities when the engine should be turned on and off, on the other hand. For the data of students’ vehicle representing the Faculty of Power and Aeronautical Engineering it has been found that such driving strategy is more effective in comparison with a constant speed strategy with the engine partly throttled, as well as a strategy resulting from optimal control theory when the engine is still active.

A Photoactivable Formaldehyde Donor with Fluorescence Monitoring Reveals Threshold to Arrest Cell Migration

2019 ◽  
Author(s):  
Lukas P Smaga ◽  
Nicholas W Pino ◽  
Gabriela E Ibarra ◽  
Vishnu Krishnamurthy ◽  
Jefferson Chan
Keyword(s):  
Wound Healing ◽  
Cell Migration ◽  
Fluorescence Enhancement ◽  
Biological Effects ◽  
Cellular Systems ◽  
Live Cells ◽  
First Report ◽  
Cell Lysate ◽  
Intracellular Release ◽  
Biological Analytes

Controlled light-mediated delivery of biological analytes enables the investigation of highly reactivity molecules within cellular systems. As many biological effects are concentration dependent, it is critical to determine the location, time, and quantity of analyte donation. In this work, we have developed the first photoactivatable donor for formaldehyde (FA). Our optimized photoactivatable donor, photoFAD-3, is equipped with a fluorescence readout that enables monitoring of FA release with a concomitant 139-fold fluorescence enhancement. Tuning of photostability and cellular retention enabled quantification of intracellular FA release through cell lysate calibration. Application of photoFAD-3 uncovered the concentration range necessary for arresting wound healing in live cells. This marks the first report where a photoactivatable donor for any analyte has been used to quantify intracellular release.

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