Sulfonate Hydrogel–siRNA Conjugate Facilitates Osteogenic Differentiation of Mesenchymal Stem Cells by Controlled Gene Silencing and Activation of BMP Signaling

2021 ◽  
Author(s):  
Soyon Kim ◽  
Jiabing Fan ◽  
Chung-Sung Lee ◽  
Chen Chen ◽  
Min Lee
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Gene Silencing ◽  
Osteogenic Differentiation ◽  
Bmp Signaling

scholarly journals SIRT6 promotes osteogenic differentiation of mesenchymal stem cells through BMP signaling

2017 ◽  
Vol 7 (1) ◽  
Author(s):  
Ping Zhang ◽  
Yunsong Liu ◽  
Yuejun Wang ◽  
Min Zhang ◽  
Longwei Lv ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Osteogenic Differentiation ◽  
Bmp Signaling

scholarly journals Bone Morphogenetic Protein-2 Signaling in the Osteogenic Differentiation of Human Bone Marrow Mesenchymal Stem Cells Induced by Pulsed Electromagnetic Fields

2020 ◽  
Vol 21 (6) ◽  
pp. 2104 ◽  
Author(s):  
Fernanda Martini ◽  
Agnese Pellati ◽  
Elisa Mazzoni ◽  
Simona Salati ◽  
Gaetano Caruso ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Osteogenic Differentiation ◽  
Bmp Signaling ◽  
Bone Morphogenetic Protein 2 ◽  
Pulsed Electromagnetic Fields ◽  
Synergistic Activity ◽  
Molecular Events ◽  
Morphogenetic Protein ◽  
Pulsed Electromagnetic

Pulsed electromagnetic fields (PEMFs) are clinically used with beneficial effects in the treatment of bone fracture healing. This is due to PEMF ability to favor the osteogenic differentiation of mesenchymal stem cells (MSCs). Previous studies suggest that PEMFs enhance the osteogenic activity of bone morphogenetic protein-2 (BMP2) which is used in various therapeutic interventions. This study investigated the molecular events associated to the synergistic activity of PEMFs and BMP2 on osteogenic differentiation. To this aim, human MSCs (hMSCs) were exposed to PEMFs (75 Hz, 1.5 mT) in combination with BMP2, upon detection of the minimal dose able to induce differentiation. Changes in the expression of BMP signaling pathway genes including receptors and ligands, as well as in the phosphorylation of BMP downstream signaling proteins, such as SMAD1/5/8 and MAPK, were analyzed. Results showed the synergistic activity of PEMFs and BMP2 on osteogenic differentiation transcription factors and markers. The PEMF effects were associated to the increase in BMP2, BMP6, and BMP type I receptor gene expression, as well as SMAD1/5/8 and p38 MAPK activation. These results increase knowledge concerning the molecular events involved in PEMF stimulation showing that PEMFs favor hMSCs osteogenic differentiation by the modulation of BMP signaling components.

TGF-β/BMP signaling pathway is involved in cerium-promoted osteogenic differentiation of mesenchymal stem cells

2013 ◽  
Vol 114 (5) ◽  
pp. 1105-1114 ◽  
Author(s):  
Dan-Dan Liu ◽  
Jin-Chao Zhang ◽  
Qun Zhang ◽  
Shu-Xiang Wang ◽  
Meng-Su Yang
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Osteogenic Differentiation ◽  
Signaling Pathway ◽  
Bmp Signaling

scholarly journals MiRNA-20a promotes osteogenic differentiation of human mesenchymal stem cells by co-regulating BMP signaling

RNA Biology ◽  
2011 ◽  
Vol 8 (5) ◽  
pp. 829-838 ◽  
Author(s):  
Jin-fang Zhang ◽  
Wei-ming Fu ◽  
Ming-liang He ◽  
Wei-dong Xie ◽  
Qing Lv ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Osteogenic Differentiation ◽  
Human Mesenchymal Stem Cells ◽  
Bmp Signaling

scholarly journals Systems biology analysis of osteogenic differentiation behavior by canine mesenchymal stem cells derived from bone marrow and dental pulp

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Sirirat Nantavisai ◽  
Trairak Pisitkun ◽  
Thanaphum Osathanon ◽  
Prasit Pavasant ◽  
Chanin Kalpravidh ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Systems Biology ◽  
Osteogenic Differentiation ◽  
Dental Pulp ◽  
Bmp Signaling ◽  
Functional Assay ◽  
Matrix Mineralization

AbstractUtilization of canine mesenchymal stem cells (cMSCs) for regenerating incorrigible bone diseases has been introduced. However, cMSCs harvested from different sources showed distinct osteogenicity. To clarify this, comparative proteomics-based systems biology analysis was used to analyze osteogenic differentiation behavior by cMSCs harvested from bone marrow and dental pulp. The results illustrated that canine dental pulp stem cells (cDPSCs) contained superior osteogenicity comparing with canine bone marrow-derived MSCs (cBM-MSCs) regarding alkaline phosphatase activity, matrix mineralization, and osteogenic marker expression. Global analyses by proteomics platform showed distinct protein clustering and expression pattern upon an in vitro osteogenic induction between them. Database annotation using Reactome and DAVID revealed contrast and unique expression profile of osteogenesis-related proteins, particularly on signaling pathways, cellular components and processes, and cellular metabolisms. Functional assay and hierarchical clustering for tracking protein dynamic change confirmed that cBM-MSCs required the presences of Wnt, transforming growth factor (TGF)-beta, and bone-morphogenetic protein (BMP) signaling, while cDPSCs mainly relied on BMP signaling presentation during osteogenic differentiation in vitro. Therefore, these findings illustrated the comprehensive data regarding an in vitro osteogenic differentiation behavior by cBM-MSCs and cDPSCs which is crucial for further mechanism study and the establishment of cMSC-based bone tissue engineering (BTE) for veterinary practice.

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