Antibacterial Vitamin K3 Carnosine Peptide-Laden Silk Fibroin Electrospun Fibers for Improvement of Skin Wound Healing in Diabetic Rats

2021 ◽  
Author(s):  
Subramani Kandhasamy ◽  
Bo Liang ◽  
Da-Peng Yang ◽  
Yiming Zeng
Keyword(s):  
Wound Healing ◽  
Silk Fibroin ◽  
Skin Wound ◽  
Diabetic Rats ◽  
Electrospun Fibers ◽  
Vitamin K3 ◽  
Skin Wound Healing

Electrospun Fibers with Plasmid bFGF Polyplex Loadings Promote Skin Wound Healing in Diabetic Rats

2011 ◽  
Vol 9 (1) ◽  
pp. 48-58 ◽  
Author(s):  
Ye Yang ◽  
Tian Xia ◽  
Fang Chen ◽  
Wei Wei ◽  
Chaoyu Liu ◽  
...  
Keyword(s):  
Wound Healing ◽  
Skin Wound ◽  
Diabetic Rats ◽  
Electrospun Fibers ◽  
Skin Wound Healing

scholarly journals Correction: Oral Administration of Linoleic Acid Induces New Vessel Formation and Improves Skin Wound Healing in Diabetic Rats

PLoS ONE ◽  
2017 ◽  
Vol 12 (5) ◽  
pp. e0179071 ◽  
Author(s):  
Hosana G. Rodrigues ◽  
Marco A. R. Vinolo ◽  
Fabio T. Sato ◽  
Juliana Magdalon ◽  
Carolina M. C. Kuhl ◽  
...  
Keyword(s):  
Wound Healing ◽  
Linoleic Acid ◽  
Oral Administration ◽  
Skin Wound ◽  
Diabetic Rats ◽  
Skin Wound Healing ◽  
Vessel Formation

scholarly journals EFFECTS OF PROBIOTICS SUPPLEMENTATION ON SKIN WOUND HEALING IN DIABETIC RATS

2020 ◽  
Vol 33 (1) ◽  
Author(s):  
Letícia Fuganti CAMPOS ◽  
Eliane TAGLIARI ◽  
Thais Andrade Costa CASAGRANDE ◽  
Lúcia de NORONHA ◽  
Antônio Carlos L. CAMPOS ◽  
...  
Keyword(s):  
Diabetes Mellitus ◽  
Wound Healing ◽  
Type I Collagen ◽  
Chronic Wounds ◽  
Skin Wound ◽  
Diabetic Rats ◽  
Control Group ◽  
Type I ◽  
Collagen Deposition ◽  
Skin Wound Healing

ABSTRACT Background: Chronic wounds in patients with Diabetes Mellitus often become incurable due to prolonged and excessive production of inflammatory cytokines. The use of probiotics modifies the intestinal microbiota and modulates inflammatory reactions. Aim: To evaluate the influence of perioperative supplementation with probiotics in the cutaneous healing process in diabetic rats. Methods: Forty-six rats were divided into four groups (C3, P3, C10, P10) according to the treatment (P=probiotic or C=control, both orally administered) and day of euthanasia, 3rd or 10th postoperative days. All rats were induced to Diabetes Mellitus 72 h before starting the experiment with alloxan. Supplementation was initiated five days before the incision and maintained until euthanasia. Scalpel incision was guided by a 2x2 cm mold and the wounds were left to heal per second-intention. The wounds were digitally measured. Collagen densitometry was done with Picrosirius Red staining. Histological parameters were analyzed by staining by H&E. Results: The contraction of the wound was faster in the P10 group which resulted in a smaller scar area (p=0.011). There was an increase in type I collagen deposition from the 3rd to the 10th postoperative day in the probiotic groups (p=0.016), which did not occur in the control group (p=0.487). The histological analysis showed a better degree of healing in the P10 group (p=0.005), with fewer polymorphonuclear (p<0.001) and more neovessels (p=0.001). Conclusions: Perioperative supplementation of probiotics stimulates skin wound healing in diabetic rats, possibly due to attenuation of the inflammatory response and increased neovascularization and type I collagen deposition.

Adipose tissue derived mesenchymal stem cell (AD-MSC) promotes skin wound healing in diabetic rats

2011 ◽  
Vol 93 (2) ◽  
pp. 228-234 ◽  
Author(s):  
Mohsen Khosravi Maharlooei ◽  
Mansooreh Bagheri ◽  
Zhabiz Solhjou ◽  
Behnam Moein Jahromi ◽  
Majid Akrami ◽  
...  
Keyword(s):  
Wound Healing ◽  
Adipose Tissue ◽  
Stem Cell ◽  
Mesenchymal Stem Cell ◽  
Skin Wound ◽  
Diabetic Rats ◽  
Skin Wound Healing

scholarly journals Oral Administration of Linoleic Acid Induces New Vessel Formation and Improves Skin Wound Healing in Diabetic Rats

PLoS ONE ◽  
2016 ◽  
Vol 11 (10) ◽  
pp. e0165115 ◽  
Author(s):  
Hosana G. Rodrigues ◽  
Marco A. R. Vinolo ◽  
Fabio T. Sato ◽  
Juliana Magdalon ◽  
Carolina M. C. Kuhl ◽  
...  
Keyword(s):  
Wound Healing ◽  
Linoleic Acid ◽  
Oral Administration ◽  
Skin Wound ◽  
Diabetic Rats ◽  
Skin Wound Healing ◽  
Vessel Formation

scholarly journals Effect of Equal Daily Doses Achieved by Different Power Densities of Low-Level Laser Therapy at 635 nm on Open Skin Wound Healing in Normal and Diabetic Rats

2014 ◽  
Vol 2014 ◽  
pp. 1-9 ◽  
Author(s):  
Róbert Kilík ◽  
Lucia Lakyová ◽  
Ján Sabo ◽  
Peter Kruzliak ◽  
Kamila Lacjaková ◽  
...  
Keyword(s):  
Wound Healing ◽  
Laser Therapy ◽  
Skin Wound ◽  
Diabetic Rats ◽  
Low Level Laser Therapy ◽  
Collagen Fibres ◽  
Skin Wound Healing ◽  
Level Laser ◽  
Significant Difference ◽  
Power Densities

Background and Objective.Despite the fact that the molecular mechanism of low-level laser therapy (LLLT) is not yet known, the exploitation of phototherapy in clinical medicine and surgery is of great interest. The present study investigates the effects of LLLT on open skin wound healing in normal and diabetic rats.Materials and Methods.Four round full-thickness skin wounds on dorsum were performed in male adult nondiabetic (n=24) and diabetic (n=24) Sprague–Dawley rats. AlGaInP (635 nm, wavelength; 5 J/cm2, daily dose) was used to deliver power densities of 1, 5, and 15 mW/cm2three times daily until euthanasia.Results.PMNL infiltration was lower in the irradiated groups (15 mW/cm2). The synthesis and organisation of collagen fibres were consecutively enhanced in the 5 mW/cm2and 15 mW/cm2groups compared to the others in nondiabetic rats. In the diabetic group the only significant difference was recorded in the ratio PMNL/Ma at 15 mW/cm2. A significant difference in the number of newly formed capillaries in the irradiated group (5, 15 mW/cm2) was recorded on day six after injury compared to the control group.Conclusion.LLLT confers a protective effect against excessive inflammatory tissue response; it stimulates neovascularization and the early formation of collagen fibres.

Bioimaging assessment and effect of skin wound healing using bone-marrow-derived mesenchymal stromal cells with the artificial dermis in diabetic rats

2008 ◽  
Vol 13 (6) ◽  
pp. 064036 ◽  
Author(s):  
Hirokazu Inoue ◽  
Takashi Murakami ◽  
Takashi Ajiki ◽  
Mayumi Hara ◽  
Yuichi Hoshino ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Wound Healing ◽  
Mesenchymal Stromal Cells ◽  
Stromal Cells ◽  
Skin Wound ◽  
Diabetic Rats ◽  
Skin Wound Healing ◽  
Artificial Dermis

scholarly journals Simple method of open skin wound healing model in corticosteroid-treated and diabetic rats: standardization of semi-quantitative and quantitative histological assessments

2008 ◽  
Vol 53 (No. 12) ◽  
pp. 652-659 ◽  
Author(s):  
P. Gal ◽  
Kilik ◽  
R ◽  
M. Mokry ◽  
B. Vidinsky ◽  
...  
Keyword(s):  
Wound Healing ◽  
Quantitative Methods ◽  
Healing Process ◽  
Skin Wound ◽  
Diabetic Rats ◽  
Control Group ◽  
Wound Healing Process ◽  
Polymorphonuclear Leucocytes ◽  
Skin Wound Healing ◽  
Simple Method

The use of a simple and reproducible model is inevitable for objective statement of the effects of external factors on wound healing. Hence, present study was conducted to establish an excisional model of skin wound healing in corticosteroid treated, and streptozotocine induced diabetic rats as well as to standardized the semi-quantitative and quantitative evaluation of selected parameters. Round full thickness skin wounds were performed on the back of male Sprague-Dawley rats. Animals were sacrificed two, six, and fourteen days after surgery. Sections were stained with hematoxylin-eosin and van Gieson. Both semi-quantitative (wound reepithelization; presence of: inflammatory cells, fibroblasts, new wessels, and collagen) and quantitative methods (polymorphonuclear leucocytes/tissue macrophages ratio, percentage of re-epithelization, area of the granulation tissue) were used to evaluate the histological changes during wound healing. As compared to the control group the wound healing process of both experimental groups was decelerated. Interestingly, wound reepithelization and angiogenesis were significantly inhibited only in the steroid rats while epithelization was accelerated in diabetic rats. In conclusion, when compared to primary sutured wound healing it can be concluded that the excisional model is more appropriate for histological assessment of the effect of various factors on wound healing. In addition, administration of corticosteroids represents simple and inexpensive model of a complex skin wound healing impairment.

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