Structure of Diisobutylene Maleic Acid Copolymer (DIBMA) and Its Lipid Particle as a “Stealth” Membrane-Mimetic for Membrane Protein Research

2021 ◽  
Author(s):  
Rong Guo ◽  
Jacob Sumner ◽  
Shuo Qian
Keyword(s):  
Membrane Protein ◽  
Maleic Acid ◽  
Acid Copolymer ◽  
Membrane Mimetic ◽  
Lipid Particle ◽  
Protein Research ◽  
Maleic Acid Copolymer

scholarly journals Diisobutylene Maleic Acid Copolymer (DIBMA) Lipid Particle: A “Stealth” Membrane Mimetic for Neutron Scattering

2020 ◽  
Author(s):  
Rong Guo ◽  
Jacob Sumner ◽  
Shuo Qian
Keyword(s):  
Membrane Proteins ◽  
Neutron Scattering ◽  
Maleic Acid ◽  
Contrast Variation ◽  
Acid Copolymer ◽  
Membrane Mimetic ◽  
Lipid Particles ◽  
Lipid Particle ◽  
Contrast Matching ◽  
Maleic Acid Copolymer

Diisobutylene maleic acid (DIBMA) has been shown to solubilize and purify membrane proteins from a native lipid bilayer into nanodiscs without the need for a detergent. To explore DIBMA lipid particles as a suitable membrane mimetic system for neutron scattering studies of membrane proteins, we measured and determined the contrast matching point of DIBMA to be ~12% (v/v) D2O—similar to that of most protiated lipid molecules, but distinct from that of regular protiated proteins, providing a natural contrast for separating neutron scattering signals. Using SANS contrast variation, we demonstrated that the scattering from the whole lipid particle can be annihilated. Further, the lipid part of the particle shows a well-defined discoidal shape with DIBMA contrast matched. These results demonstrate that the DIBMA lipid particle is an outstanding “stealth” membrane mimetic for membrane proteins.<br>

scholarly journals Diisobutylene Maleic Acid Copolymer (DIBMA) Lipid Particle: A “Stealth” Membrane Mimetic for Neutron Scattering

2020 ◽  
Author(s):  
Rong Guo ◽  
Jacob Sumner ◽  
Shuo Qian
Keyword(s):  
Membrane Proteins ◽  
Neutron Scattering ◽  
Maleic Acid ◽  
Contrast Variation ◽  
Acid Copolymer ◽  
Membrane Mimetic ◽  
Lipid Particles ◽  
Lipid Particle ◽  
Contrast Matching ◽  
Maleic Acid Copolymer

Diisobutylene maleic acid (DIBMA) has been shown to solubilize and purify membrane proteins from a native lipid bilayer into nanodiscs without the need for a detergent. To explore DIBMA lipid particles as a suitable membrane mimetic system for neutron scattering studies of membrane proteins, we measured and determined the contrast matching point of DIBMA to be ~12% (v/v) D2O—similar to that of most protiated lipid molecules, but distinct from that of regular protiated proteins, providing a natural contrast for separating neutron scattering signals. Using SANS contrast variation, we demonstrated that the scattering from the whole lipid particle can be annihilated. Further, the lipid part of the particle shows a well-defined discoidal shape with DIBMA contrast matched. These results demonstrate that the DIBMA lipid particle is an outstanding “stealth” membrane mimetic for membrane proteins.<br>

Functional Integrity of Membrane Protein Rhodopsin Solubilized by Styrene-Maleic Acid Copolymer

2021 ◽  
Author(s):  
Stephanie G. Pitch ◽  
Weekie Yao ◽  
Istvan Szundi ◽  
Jonathan Fay ◽  
Eefei Chen ◽  
...  
Keyword(s):  
Membrane Protein ◽  
Maleic Acid ◽  
Functional Integrity ◽  
Acid Copolymer ◽  
Maleic Acid Copolymer

Comparative Analysis of Maleic Acid Copolymer-Based Lipid Nanodiscs Reveals Preferential Lipid and Protein Solubilization

2020 ◽  
Author(s):  
Marta Barniol-Xicota ◽  
Steven Verhelst
Keyword(s):  
Comparative Analysis ◽  
Membrane Proteins ◽  
Membrane Protein ◽  
Protein Content ◽  
Lipid Bilayer ◽  
Maleic Acid ◽  
Chemical Properties ◽  
Acid Copolymer ◽  
Lipid Nanodiscs ◽  
Maleic Acid Copolymer

Membrane proteins are key in a large number of physiological and pathological processes. Their study often involves a prior detergent solubilization step, which strips away the membrane and can jeopardize membrane protein integrity. A recent alternative to detergents encompasses maleic acid based copolymers (xMAs), which disrupt the lipid bilayer and form lipid protein nanodiscs (xMALPs) soluble in aqueous buffer. Although xMALPs are often referred to as native nanodiscs, little is known about the resemblance of their lipid and protein content to the native bilayer. Here we have analyzed prokaryotic and eukaryotic xMALPs using lipidomics and in-gel analysis. Our results show that the xMALPs content varies with the chemical properties of the used xMA and that some of these nanodiscs are less native than initially thought.<br>

scholarly journals Characterizing the Structure of Styrene Maleic Acid Copolymer Lipid Nanoparticles (SMALPS) Using Raft Polymerization for Membrane Protein Spectroscopic Studies

2020 ◽  
Vol 118 (3) ◽  
pp. 361a-362a
Author(s):  
Benjamin D. Harding ◽  
Gunjan Dixit ◽  
Kevin M. Burridge ◽  
Gary A. Lorigan ◽  
Indra D. Sahu ◽  
...  
Keyword(s):  
Membrane Protein ◽  
Maleic Acid ◽  
Raft Polymerization ◽  
Lipid Nanoparticles ◽  
Spectroscopic Studies ◽  
Acid Copolymer ◽  
Maleic Acid Copolymer

scholarly journals Characterizing the Structure of Styrene-Maleic Acid Copolymer-Lipid Nanoparticles (SMALPS) using Raft Polymerization for Membrane Protein Spectroscopic Studies

2019 ◽  
Vol 116 (3) ◽  
pp. 517a
Author(s):  
Benjamin D. Harding ◽  
Gunjan Dixit ◽  
Kevin M. Burridge ◽  
Indra D. Sahu ◽  
Carole Dabney-Smith ◽  
...  
Keyword(s):  
Membrane Protein ◽  
Maleic Acid ◽  
Raft Polymerization ◽  
Lipid Nanoparticles ◽  
Spectroscopic Studies ◽  
Acid Copolymer ◽  
Maleic Acid Copolymer

scholarly journals Comparative Analysis of Maleic Acid Copolymer-Based Lipid Nanodiscs Reveals Preferential Lipid and Protein Solubilization

2020 ◽  
Author(s):  
Marta Barniol-Xicota ◽  
Steven Verhelst
Keyword(s):  
Comparative Analysis ◽  
Membrane Proteins ◽  
Membrane Protein ◽  
Protein Content ◽  
Lipid Bilayer ◽  
Maleic Acid ◽  
Chemical Properties ◽  
Acid Copolymer ◽  
Lipid Nanodiscs ◽  
Maleic Acid Copolymer

Membrane proteins are key in a large number of physiological and pathological processes. Their study often involves a prior detergent solubilization step, which strips away the membrane and can jeopardize membrane protein integrity. A recent alternative to detergents encompasses maleic acid based copolymers (xMAs), which disrupt the lipid bilayer and form lipid protein nanodiscs (xMALPs) soluble in aqueous buffer. Although xMALPs are often referred to as native nanodiscs, little is known about the resemblance of their lipid and protein content to the native bilayer. Here we have analyzed prokaryotic and eukaryotic xMALPs using lipidomics and in-gel analysis. Our results show that the xMALPs content varies with the chemical properties of the used xMA and that some of these nanodiscs are less native than initially thought.<br>

scholarly journals Development of discoidal phospholipid bilayer nanoparticles with styrene maleic acid copolymer for diagnosis and therapy of intractable cancers

2021 ◽  
Vol 96-97 ◽  
pp. S83
Author(s):  
Masayuki Munekane ◽  
Wakana Yamaguchi ◽  
Kohei Sano ◽  
Toshihide Yamasaki ◽  
Masafumi Tanaka ◽  
...  
Keyword(s):  
Maleic Acid ◽  
Phospholipid Bilayer ◽  
Diagnosis And Therapy ◽  
Acid Copolymer ◽  
Maleic Acid Copolymer
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