Sensitive Fluorescence Aptasensor Based on Hybridization Chain Reaction with Upconversion Nanoparticles by Triplex DNA Formation for Bisphenol A Detection

2020 ◽  
Author(s):  
Yu Wang ◽  
Xudong Zhao ◽  
Bingyang Huo ◽  
Shuyue Ren ◽  
Jialei Bai ◽  
...  
Keyword(s):  
Bisphenol A ◽  
Triplex Dna ◽  
Upconversion Nanoparticles ◽  
Hybridization Chain Reaction ◽  
Chain Reaction ◽  
Bisphenol A Detection

An Ag+-stabilized triplex DNA molecular switch controlled hybridization chain reaction

2017 ◽  
Vol 60 (12) ◽  
pp. 1575-1580 ◽  
Author(s):  
Yanwei Luo ◽  
Ruimin Li ◽  
Jing Wang ◽  
Manjun Zhang ◽  
Li Zou ◽  
...  
Keyword(s):  
Molecular Switch ◽  
Triplex Dna ◽  
Hybridization Chain Reaction ◽  
Chain Reaction

scholarly journals An enzyme free fluorescence resonance transfer strategy based on hybrid chain reaction and triplex DNA for Vibrio parahaemolyticus

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Xiao-Hui Tan ◽  
Yu-Bin Li ◽  
Yan Liao ◽  
Hua-Zhong Liu
Keyword(s):  
Vibrio Parahaemolyticus ◽  
Molecular Beacon ◽  
Close Contact ◽  
Double Helix ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Triplex Dna ◽  
Hybridization Chain Reaction ◽  
Chain Reaction ◽  
Fluorescence Resonance

AbstractIn this work, an enzyme-free fluorescence resonance energy transfer (FRET) strategy was established for rapid and specific detection of the DNA sequence from Vibrio parahaemolyticus (VP) using hybridization chain reaction (HCR) amplification and triplex DNA. The triplex forming oligonucleotide (TFO) was labelled with carboxyfluorescein (FAM) as fluorescence donor, and hairpin sequence H1 was labelled by tetramethylrhodamine (TAMRA) as fluorescence receptor. In the present target VP DNA, the hairpin structure of molecular beacon (MB) was opened, the free end was released and hybridized with H1-TAMRA, and the HCR reaction was triggered by the alternate supplementation of H1-TAMRA and H2 to produce the notch double helix analogue. After the addition of TFO-FAM, a triplex structure was formed between HCR products (H1-TAMRA/H2) and TFO-FAM. A close contact between the donor and the receptor resulted in FRET. Under the optimal conditions, the fluorescence quenching value was inversely proportional to the concentration of target VP DNA in the range of 0.1–50 nmol L−1, and the detection limit was 35 pmol L−1.

A new method for the in situ assay of α-glucosidase activity and inhibitor screening through an enzyme substrate-mediated DNA hybridization chain reaction

2019 ◽  
Vol 43 (24) ◽  
pp. 9458-9465
Author(s):  
Xiquan Yue ◽  
Lihong Su ◽  
Xu Chen ◽  
Junfeng Liu ◽  
Longpo Zheng ◽  
...  
Keyword(s):  
Small Molecule ◽  
Dna Hybridization ◽  
New Method ◽  
Hybridization Chain Reaction ◽  
Chain Reaction ◽  
Inhibitor Screening ◽  
Glucosidase Activity ◽  
Enzyme Substrate ◽  
In Situ Assay

The strategy is based on small molecule-mediated hybridization chain reaction.

Ultrasensitive ratiometric detection of Pb2+ using DNA tetrahedron-mediated hyperbranched hybridization chain reaction

2021 ◽  
Vol 1147 ◽  
pp. 170-177
Author(s):  
Pingping Ji ◽  
Guimei Han ◽  
Yan Huang ◽  
Hongxin Jiang ◽  
Qiwen Zhou ◽  
...  
Keyword(s):  
Hybridization Chain Reaction ◽  
Chain Reaction ◽  
Ratiometric Detection ◽  
Dna Tetrahedron

scholarly journals Spatiotemporally programmable cascade hybridization of hairpin DNA in polymeric nanoframework for precise siRNA delivery

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Feng Li ◽  
Wenting Yu ◽  
Jiaojiao Zhang ◽  
Yuhang Dong ◽  
Xiaohui Ding ◽  
...  
Keyword(s):  
Steric Effect ◽  
Sirna Delivery ◽  
Dna Nanotechnology ◽  
Hybridization Chain Reaction ◽  
Dna Nanostructures ◽  
Chain Reaction ◽  
Dna Hairpins ◽  
In Vivo Experiments ◽  
Dna And Rna

AbstractDNA nanostructures have been demonstrated as promising carriers for gene delivery. In the carrier design, spatiotemporally programmable assembly of DNA under nanoconfinement is important but has proven highly challenging due to the complexity–scalability–error of DNA. Herein, a DNA nanotechnology-based strategy via the cascade hybridization chain reaction (HCR) of DNA hairpins in polymeric nanoframework has been developed to achieve spatiotemporally programmable assembly of DNA under nanoconfinement for precise siRNA delivery. The nanoframework is prepared via precipitation polymerization with Acrydite-DNA as cross-linker. The potential energy stored in the loops of DNA hairpins can overcome the steric effect in the nanoframework, which can help initiate cascade HCR of DNA hairpins and achieve efficient siRNA loading. The designer tethering sequence between DNA and RNA guarantees a triphosadenine triggered siRNA release specifically in cellular cytoplasm. Nanoframework provides stability and ease of functionalization, which helps address the complexity–scalability–error of DNA. It is exemplified that the phenylboronate installation on nanoframework enhanced cellular uptake and smoothed the lysosomal escape. Cellular results show that the siRNA loaded nanoframework down-regulated the levels of relevant mRNA and protein. In vivo experiments show significant therapeutic efficacy of using siPLK1 loaded nanoframework to suppress tumor growth.

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