Mesenchymal Stem Cell and Oxygen Modulate the Cocultured Endothelial Cells in the Presence of Magnesium Degradation Products

2021 ◽  
Vol 4 (3) ◽  
pp. 2398-2407
Author(s):  
Lei Xu ◽  
Regine Willumeit-Römer ◽  
Bérengère J. C. Luthringer-Feyerabend
Keyword(s):  
Endothelial Cells ◽  
Stem Cell ◽  
Mesenchymal Stem Cell ◽  
Degradation Products

scholarly journals Synergy of molecularly mobile polyrotaxane surfaces with endothelial cell co-culture for mesenchymal stem cell mineralization

RSC Advances ◽  
2021 ◽  
Vol 11 (30) ◽  
pp. 18685-18692
Author(s):  
Hiroki Masuda ◽  
Yoshinori Arisaka ◽  
Masahiro Hakariya ◽  
Takanori Iwata ◽  
Tetsuya Yoda ◽  
...  
Keyword(s):  
Stem Cells ◽  
Endothelial Cells ◽  
Mesenchymal Stem Cells ◽  
Stem Cell ◽  
Endothelial Cell ◽  
Mesenchymal Stem Cell ◽  
Molecular Mobility ◽  
Culture System

Molecular mobility of polyrotaxane surfaces promoted mineralization in a co-culture system of mesenchymal stem cells and endothelial cells.

scholarly journals Effects of magnesium degradation products on mesenchymal stem cell fate and osteoblastogenesis

Gene ◽  
2016 ◽  
Vol 575 (1) ◽  
pp. 9-20 ◽  
Author(s):  
Bérengère J.C. Luthringer ◽  
Regine Willumeit-Römer
Keyword(s):  
Stem Cell ◽  
Mesenchymal Stem Cell ◽  
Cell Fate ◽  
Degradation Products ◽  
Stem Cell Fate

Mesenchymal stem cell-conditioned media ameliorate diabetic endothelial dysfunction by improving mitochondrial bioenergetics via the Sirt1/AMPK/PGC-1α pathway

2016 ◽  
Vol 130 (23) ◽  
pp. 2181-2198 ◽  
Author(s):  
Yujia Yuan ◽  
Meimei Shi ◽  
Lan Li ◽  
Jingping Liu ◽  
Bo Chen ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Endothelial Cells ◽  
Stem Cell ◽  
Protein Kinase ◽  
Endothelial Dysfunction ◽  
Mesenchymal Stem Cell ◽  
Mitochondrial Biogenesis ◽  
Mitochondrial Function ◽  
Mitochondrial Bioenergetics ◽  
Sirt1 Expression

Vasculopathy is a major complication of diabetes. Impaired mitochondrial bioenergetics and biogenesis due to oxidative stress are a critical causal factor for diabetic endothelial dysfunction. Sirt1, an NAD+-dependent enzyme, is known to play an important protective role through deacetylation of many substrates involved in oxidative phosphorylation and reactive oxygen species generation. Mesenchymal stem cell-conditioned medium (MSC-CM) has emerged as a promising cell-free therapy due to the trophic actions of mesenchymal stem cell (MSC)-secreted molecules. In the present study, we investigated the therapeutic potential of MSC-CMs in diabetic endothelial dysfunction, focusing on the Sirt1 signalling pathway and the relevance to mitochondrial function. We found that high glucose-stimulated MSC-CM attenuated several glucotoxicity-induced processes, oxidative stress and apoptosis of endothelial cells of the human umbilical vein. MSC-CM perfusion in diabetic rats ameliorated compromised aortic vasodilatation and alleviated oxidative stress in aortas. We further demonstrated that these effects were dependent on improved mitochondrial function and up-regulation of Sirt1 expression. MSC-CMs activated the phosphorylation of phosphoinositide 3-kinase (PI3K) and protein kinase B (Akt), leading to direct interaction between Akt and Sirt1, and subsequently enhanced Sirt1 expression. In addition, both MSC-CM and Sirt1 activation could increase the expression of peroxisome proliferator-activated receptor γ co-activator-1α (PGC-1α), as well as increase the mRNA expression of its downstream, mitochondrial, biogenesis-related genes. This indirect regulation was mediated by activation of AMP-activated protein kinase (AMPK). Overall our findings indicated that MSC-CM had protective effects on endothelial cells, with respect to glucotoxicity, by ameliorating mitochondrial dysfunction via the PI3K/Akt/Sirt1 pathway, and Sirt1 potentiated mitochondrial biogenesis, through the Sirt1/AMPK/PGC-1α pathway.

Regulation of Mesenchymal Stem Cell Activity by Endothelial Cells

2011 ◽  
Vol 20 (3) ◽  
pp. 391-403 ◽  
Author(s):  
Fatima A. Saleh ◽  
Muna Whyte ◽  
Peter Ashton ◽  
Paul G. Genever
Keyword(s):  
Endothelial Cells ◽  
Stem Cell ◽  
Mesenchymal Stem Cell ◽  
Cell Activity ◽  
Stem Cell Activity

Declined presentation mesenchymal stem cell-like cell mediated hematopoietic stem cell generation from non-hemogenic endothelial cells

2017 ◽  
Vol 53 ◽  
pp. S118-S119
Author(s):  
Vashe Chandrakanthan ◽  
Young Chan Kang ◽  
Kathy Knezevic ◽  
Qiao Qiao ◽  
Rema Oliver ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Stem Cell ◽  
Mesenchymal Stem Cell ◽  
Hematopoietic Stem Cell ◽  
Cell Generation ◽  
Hematopoietic Stem
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