A Cell Membrane Fluorogenic Probe for Gram-Positive Bacteria Imaging and Real-Time Tracking of Bacterial Viability

2020 ◽  
Author(s):  
Weiwei Liu ◽  
Ruihua Li ◽  
Fei Deng ◽  
Chunyu Yan ◽  
Xuelian Zhou ◽  
...  
Keyword(s):  
Cell Membrane ◽  
Real Time ◽  
Gram Positive ◽  
Bacterial Viability ◽  
Gram Positive Bacteria ◽  
Fluorogenic Probe ◽  
A Cell ◽  
Real Time Tracking

scholarly journals Promysalin is a salicylate-containing antimicrobial with a cell-membrane-disrupting mechanism of action on Gram-positive bacteria

2017 ◽  
Vol 7 (1) ◽  
Author(s):  
Rahul D. Kaduskar ◽  
Giulia Della Scala ◽  
Zaaima J. H. Al Jabri ◽  
Stefania Arioli ◽  
Loana Musso ◽  
...  
Keyword(s):  
Cell Membrane ◽  
Mechanism Of Action ◽  
Gram Positive ◽  
Gram Positive Bacteria ◽  
A Cell

scholarly journals L-Ficolin Specifically Binds to Lipoteichoic Acid, a Cell Wall Constituent of Gram-Positive Bacteria, and Activates the Lectin Pathway of Complement

2004 ◽  
Vol 172 (2) ◽  
pp. 1198-1202 ◽  
Author(s):  
Nicholas J. Lynch ◽  
Silke Roscher ◽  
Thomas Hartung ◽  
Siegfried Morath ◽  
Misao Matsushita ◽  
...  
Keyword(s):  
Cell Wall ◽  
Lipoteichoic Acid ◽  
Lectin Pathway ◽  
Gram Positive ◽  
Gram Positive Bacteria ◽  
Cell Wall Constituent ◽  
A Cell

A purpurin-peptide derivative for selective killing of Gram-positive bacteria via insertion into cell membrane

2016 ◽  
Vol 4 (28) ◽  
pp. 4855-4861 ◽  
Author(s):  
Jin Zhou ◽  
Guo-Bin Qi ◽  
Hao Wang
Keyword(s):  
Cell Membrane ◽  
Gram Positive ◽  
Gram Positive Bacteria ◽  
Peptide Derivative

A purpurin-peptide derivative was used for discriminating and killing bacteria based on the different surface components of bacteria.

Fmoc-phenylalanine displays antibacterial activity against Gram-positive bacteria in gel and solution phases

Soft Matter ◽  
2018 ◽  
Vol 14 (12) ◽  
pp. 2234-2244 ◽  
Author(s):  
Avinash Y. Gahane ◽  
Pritish Ranjan ◽  
Virender Singh ◽  
Raj K. Sharma ◽  
Neeraj Sinha ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Cell Membrane ◽  
Osmotic Stress ◽  
Gram Positive ◽  
Gram Positive Bacteria

Fmoc-F incurs antibacterial activity by inducing oxidative and osmotic stress and disrupting cell membrane through the formation of micelle-like structures.

scholarly journals Methods for Confirming the Gram Reaction of Gram-variable Bacillus Species Isolated from Tobacco

2000 ◽  
Vol 19 (2) ◽  
pp. 97-101
Author(s):  
A Morin ◽  
N Poirier ◽  
S Vallee ◽  
A Porter
Keyword(s):  
Cell Wall ◽  
Bacillus Species ◽  
Gram Negative Bacteria ◽  
Gram Positive ◽  
Gram Negative ◽  
Gram Positive Bacteria ◽  
A Cell ◽  
Staining Techniques ◽  
Susceptibility Tests ◽  
Hydrolysis Of

AbstractBacillusis a predominant genus of bacteria isolated from tobacco. The Gram stain is the most commonly used and most important of all diagnostic staining techniques in microbiology. In order to help confirm the Gram positivity ofBacillusisolates from tobacco, three methods using the chemical differences of the cell wall and membrane of Gram-positive and Gram-negative bacteria were investigated: the KOH (potassium hydroxide), the LANA (L-alanine-4-nitroanilide), and the vancomycin susceptibility tests. When colonies of Gram-negative bacteria are treated with 3% KOH solution, a slimy suspension is produced, probably due to destruction of the cell wall and liberation of deoxyribonucleic acid (DNA). Gram-positive cell walls resist KOH treatment. The LANA test reveals the presence of a cell wall aminopeptidase that hydrolyzes the L-alanine-4-nitroanilide in Gram-negative bacteria. This enzyme is absent in Gram-positive bacteria. Vancomycin is a glycopeptide antibiotic inhibiting the cell wall peptido-glycan synthesis of Gram-positive microorganisms. Absence of lysis with KOH, absence of hydrolysis of LANA, and susceptibility to vancomycin were used with the Gram reaction to confirm the Gram positivity of variousBacillusspecies isolated from tobacco.B. laevolacticusexcepted, all Bacillus species tested showed negative reactions to KOH and LANA tests, and all species were susceptible to vancomycin (5 and 30 µg).

scholarly journals Rapid Phenotypic Detection of Microbial Resistance in Gram-Positive Bacteria by a Real-Time Laser Scattering Method†

2017 ◽  
Vol 8 ◽  
Author(s):  
Evgeny A. Idelevich ◽  
Matthias Hoy ◽  
Dennis Görlich ◽  
Dennis Knaack ◽  
Barbara Grünastel ◽  
...  
Keyword(s):  
Real Time ◽  
Scattering Method ◽  
Gram Positive ◽  
Laser Scattering ◽  
Microbial Resistance ◽  
Gram Positive Bacteria

scholarly journals Detecting Bacterial Cell Viability in Few µL Solutions from Impedance Measurements on Silicon-Based Biochips

2021 ◽  
Vol 22 (7) ◽  
pp. 3541
Author(s):  
Vinayak J. Bhat ◽  
Sahitya V. Vegesna ◽  
Mahdi Kiani ◽  
Xianyue Zhao ◽  
Daniel Blaschke ◽  
...  
Keyword(s):  
Live Cells ◽  
Gram Positive ◽  
Impedance Measurements ◽  
Lysinibacillus Sphaericus ◽  
Gram Positive Bacteria ◽  
High Throughput Drug Screening ◽  
A Cell ◽  
Bacterial Cell Viability ◽  
Measured Impedance ◽  
Silicon Based

Using two different types of impedance biochips (PS5 and BS5) with ring top electrodes, a distinct change of measured impedance has been detected after adding 1–5 µL (with dead or live Gram-positive Lysinibacillus sphaericus JG-A12 cells to 20 µL DI water inside the ring top electrode. We relate observed change of measured impedance to change of membrane potential of L. sphaericus JG-A12 cells. In contrast to impedance measurements, optical density (OD) measurements cannot be used to distinguish between dead and live cells. Dead L. sphaericus JG-A12 cells have been obtained by adding 0.02 mg/mL of the antibiotics tetracycline and 0.1 mg/mL chloramphenicol to a batch with OD0.5 and by incubation for 24 h, 30 °C, 120 rpm in the dark. For impedance measurements, we have used batches with a cell density of 25.5 × 108 cells/mL (OD8.5) and 270.0 × 108 cells/mL (OD90.0). The impedance biochip PS5 can be used to detect the more resistive and less capacitive live L. sphaericus JG-A12 cells. Also, the impedance biochip BS5 can be used to detect the less resistive and more capacitive dead L. sphaericus JG-A12 cells. An outlook on the application of the impedance biochips for high-throughput drug screening, e.g., against multi-drug-resistant Gram-positive bacteria, is given.

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