None-Loss Target Release of Biomimetic CaCO3 Nanocomposites for Screening Bioactive Components and Target Proteins

2020 ◽  
Author(s):  
Yingying Tang ◽  
Wenhui Qian ◽  
Bei Zhang ◽  
Wenya Liu ◽  
Xuetong Sun ◽  
...  
Keyword(s):  
Bioactive Components ◽  
Target Proteins

scholarly journals GEPSI: A Gene Expression Profile Similarity-Based Identification Method of Bioactive Components in Traditional Chinese Medicine Formula

2018 ◽  
Vol 2018 ◽  
pp. 1-9
Author(s):  
Baixia Zhang ◽  
Shuaibing He ◽  
Chenyang Lv ◽  
Yanling Zhang ◽  
Yun Wang
Keyword(s):  
Gene Expression ◽  
Molecular Docking ◽  
Chinese Medicine ◽  
Traditional Chinese Medicine ◽  
Small Molecule ◽  
Expression Profiles ◽  
Gene Expression Profiles ◽  
Bioactive Components ◽  
Target Proteins ◽  
Small Molecule Drugs

The identification of bioactive components in traditional Chinese medicine (TCM) is an important part of the TCM material foundation research. Recently, molecular docking technology has been extensively used for the identification of TCM bioactive components. However, target proteins that are used in molecular docking may not be the actual TCM target. For this reason, the bioactive components would likely be omitted or incorrect. To address this problem, this study proposed the GEPSI method that identified the target proteins of TCM based on the similarity of gene expression profiles. The similarity of the gene expression profiles affected by TCM and small molecular drugs was calculated. The pharmacological action of TCM may be similar to that of small molecule drugs that have a high similarity score. Indeed, the target proteins of the small molecule drugs could be considered TCM targets. Thus, we identified the bioactive components of a TCM by molecular docking and verified the reliability of this method by a literature investigation. Using the target proteins that TCM actually affected as targets, the identification of the bioactive components was more accurate. This study provides a fast and effective method for the identification of TCM bioactive components.

Comparative Study of Contents of Several Bioactive Components in Fruiting Bodies and Mycelia of Culinary-Medicinal Mushrooms

2013 ◽  
Vol 15 (3) ◽  
pp. 315-323 ◽  
Author(s):  
Shin-Yi Lin ◽  
Yu-Kai Chen ◽  
Hui-Tzu Yu ◽  
Gayane S. Barseghyan ◽  
Mikheil D. Asatiani ◽  
...  
Keyword(s):  
Comparative Study ◽  
Fruiting Bodies ◽  
Bioactive Components ◽  
Medicinal Mushrooms

Effect of Culture Time on the Bioactive Components in the Fruit Bodies of Caterpillar Mushroom, Cordyceps militaris CM-H0810 (Ascomycetes)

2019 ◽  
Vol 21 (11) ◽  
pp. 1107-1114
Author(s):  
Lina Zhu ◽  
Hongxia Zhang ◽  
Yangfang Liu ◽  
Jing-Song Zhang ◽  
Xinhua Gao ◽  
...  
Keyword(s):  
Cordyceps Militaris ◽  
Bioactive Components ◽  
Culture Time

Determination of Antioxidant Activities and Bioactive Components of Foeniculum vulgare Mill. (Samonsabar)

2019 ◽  
Vol 30 (3) ◽  
pp. 16-22
Keyword(s):  
Antioxidant Activities ◽  
Biological Activities ◽  
Scientific Information ◽  
Ethanolic Extract ◽  
The Body ◽  
World Health ◽  
Gas Chromatography Mass Spectrometry ◽  
Molecular Formula ◽  
Bioactive Components ◽  
Foeniculum Vulgare

World Health Organization (WHO) estimated that 80% of the population of developing countries use traditional medicines, mostly natural plant products, for their primary health care needs. In the past few decades, the medicinal value of plants has been assumed more important dimension owing largely to the discovery that extracts from plants contain not only primary metabolites but also a diverse array of secondary metabolites with antioxidant potential. Medicinal plants are potential sources of natural compounds with biological activities and therefore attract the attention of researchers worldwide. Antioxidants are vital substances which possess ability to protect the body from damage due to free radical-induced oxidative stress. The purpose of current study was to determine the antioxidant activities and bioactive components of Foeniculum vulgare (fennel) (Samonsabar) seeds by using UV Visible Spectrophotometer (UV-Vis) and Gas Chromatography-Mass Spectrometry (GC-MS). Aqueous extract of fennel seeds showed more antioxidant activity (IC50: 0.28 ug/ml) than ethanolic extract (IC50: 0.83 ug/ml) and comparable to standard antioxidant, ascorbic acid (IC50: 0.59 ug/ml). GC-MS analysis was fruitful in identification of compounds based on peak area, retention time, molecular formula, molecular weight, MS Fragmentions and pharmacological actions. Ten bioactive phytochemical compounds from aqueous extracts and 11 from ethanolic extract of fennel seeds were identified. These findings indicated that fennel seeds are potential to provide preventive properties against oxidative damage. These results will give scientific information for quality control of indigenous drug to herbal medicine users and local practitioners using fennel for different types of ailments

Properties and Biotechnological Application of mutant Derivatives of Mini-intein PRP8 from Penicillium chrysogenum with Improved Control of C-terminal Processing

2019 ◽  
Vol 35 (6) ◽  
pp. 91-101
Author(s):  
F.A. Klebanov ◽  
S.E. Cheperegin ◽  
D.G. Kozlov
Keyword(s):  
Penicillium Chrysogenum ◽  
Protein Denaturation ◽  
Biologically Active ◽  
Cultivation Temperature ◽  
Target Proteins ◽  
E Coli ◽  
Complete Inactivation ◽  
Target Molecules ◽  
Proteins And Peptides

Mutant variants of mini-intein PRP8 from Penicillium chrysogenum (Int4b) with improved control of C-terminal processing were characterized. The presented variants can serve as a basis for self-removed polypeptide tags capable of carrying an affine label and allowing to optimize the process of obtaining target proteins and peptides in E. coli cells. They allow to synthesize target molecules in the composition of soluble and insoluble hybrid proteins (fusions), provide their afnne purification, autocatalytic processing and obtaining mature target products. The presented variants have a number of features in comparison with the known prototypes. In particular the mutant mini-intein Int4bPRO, containing the L93P mutation, has temperature-dependent properties. At cultivation temperature below 30 °C it allows the production of target molecules as part of soluble fusions, but after increasing of cultivation temperature to 37 °C it directs the most of synthesized fusions into insoluble intracellular aggregates. The transition of Int4bPRO into insoluble form is accompanied by complete inactivation of C-terminal processing. Further application of standard protein denaturation-renaturation procedures enable efficiently reactivate Int4bPRO and to carry out processing of its fusions in vitro. Two other variants, Int4b56 and Int4b36, containing a point mutation T62N or combination of mutations D144N and L146T respectively, have a reduced rate of C-terminal processing. Their use in E. coli cells allows to optimize the biosynthesis of biologically active target proteins and peptides in the composition of soluble fusions, suitable for afnne purification and subsequent intein-dependent processing without the use of protein denaturation-renaturation procedures. intein, fusion, processing, processing rate, gelonin The work was supported within the framework of the State Assignment no. 595-00003-19 PR.

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