Parylene-Based Porous Scaffold with Functionalized Encapsulation of Platelet-Rich Plasma and Living Stem Cells for Tissue Engineering Applications

2020 ◽  
Vol 3 (10) ◽  
pp. 7193-7201
Author(s):  
Chih-Yu Wu ◽  
Chin-Lin Guo ◽  
Yen-Ching Yang ◽  
Chao-Wei Huang ◽  
Jun-Yu Zeng ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Porous Scaffold ◽  
Platelet Rich Plasma ◽  
Engineering Applications

Regenerative Medicine: Applications and Development in Urology

2007 ◽  
Vol 74 (4) ◽  
pp. 197-205
Author(s):  
F. Pinto ◽  
A. Calarco ◽  
A. Brescia ◽  
E. Sacco ◽  
A. D'addessi ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Regenerative Medicine ◽  
Cell Transplantation ◽  
Materials Science ◽  
Experimental Studies ◽  
Embryonic Stem ◽  
Human Embryos ◽  
Engineering Applications

Purpose Congenital abnormalities and acquired disorders can lead to organ damage and loss. Nowadays, transplantation represents the only effective treatment option. However, there is a marked decrease in the number of organ donors, which is even yearly worsening due to the population aging. The regenerative medicine represents a realistic option that allows to restore and maintain the normal functions of tissues and organs. This article reviews the principles of regenerative medicine and the recent advances with regard to its application to the genitourinary tract. Recent findings The field of regenerative medicine involves different areas of technology, such as tissue engineering, stem cells and cloning. Tissue engineering involves the field of cell transplantation, materials science and engineering in order to create functional replacement tissues. Stem cells and cloning permit the extraction of pluripotent, embryonic stem cells offering a potentially limitless source of cells for tissue engineering applications. Most current strategies for tissue engineering depend upon a sample of autologous cells from the patient's diseased organ. Biopsies from patients with extensive end-stage organ failure, however, may not yield enough normal cells. In these situations, stem cells are envisaged as being an alternative source. Stem cells can be derived from discarded human embryos (human embryonic stem cells), from fetal tissue or from adult sources (bone marrow, fat, skin). Therapeutic cloning offers a potentially limitless source of cells for tissue engineering applications. Regenerative medicine and tissue engineering scientists have increasingly applied the principles of cell transplantation, materials science and bioengineering to construct biological substitutes that will restore and maintain normal function in urological diseased and injured tissues such as kidney, ureter, bladder, urethra and penis. Conclusions Regenerative medicine offers several applications in acquired and congenital genitourinary diseases. Tissue engineering, stem cells and, mostly, cloning have been applied in experimental studies with excellent results. Few preliminary human applications have been developed with promising results.

Bioengineered Teeth from Cultured Rat Tooth Bud Cells

2004 ◽  
Vol 83 (7) ◽  
pp. 523-528 ◽  
Author(s):  
M.T. Duailibi ◽  
S.E. Duailibi ◽  
C.S. Young ◽  
J.D. Bartlett ◽  
J.P. Vacanti ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Engineering Applications ◽  
Adult Rat ◽  
Dental Tissues ◽  
Biodegradable Scaffolds ◽  
Bioengineered Tooth ◽  
Tooth Tissue

The recent bioengineering of complex tooth structures from pig tooth bud tissues suggests the potential for the regeneration of mammalian dental tissues. We have improved tooth bioengineering methods by comparing the utility of cultured rat tooth bud cells obtained from three- to seven-day post-natal (dpn) rats for tooth-tissue-engineering applications. Cell-seeded biodegradable scaffolds were grown in the omenta of adult rat hosts for 12 wks, then harvested. Analyses of 12-week implant tissues demonstrated that dissociated 4-dpn rat tooth bud cells seeded for 1 hr onto PGA or PLGA scaffolds generated bioengineered tooth tissues most reliably. We conclude that tooth-tissue-engineering methods can be used to generate both pig and rat tooth tissues. Furthermore, our ability to bioengineer tooth structures from cultured tooth bud cells suggests that dental epithelial and mesenchymal stem cells can be maintained in vitro for at least 6 days.

scholarly journals Tissue Engineering Scaffold Materials to Repair Sports Cartilage Injury

2021 ◽  
Vol 2021 ◽  
pp. 1-9
Author(s):  
Mo Xing
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Cell Suspension ◽  
Cell Density ◽  
Porous Scaffold ◽  
Cartilage Damage ◽  
Tissue Engineering Scaffold ◽  
Cartilage Injury ◽  
Culture Bottle ◽  
Scaffold Materials

With the continued development of sports in China, sports sometimes cause cartilage damage. The purpose of this research is to study the tissue engineering scaffold material for sports cartilage damage repair. In this study, mesenchymal rat bone marrow stem cells (to put it simply, stem cells are a type of cell with unlimited or immortal self-renewal capacity, capable of producing at least one type of highly differentiated progeny cells) were obtained by the total bone wash method. The cells were inoculated into the cell culture bottle. When the primary cultured cells proliferated to about 80% of the culture bottle area, the cells were digested with trypsin to open the cell link, then the medium containing 10% serum was added to terminate the cell digestion, and then the passage expansion was carried out according to the cell density. PLGA/NHA and PLGA were heated to 65°C under ultrasonic vibration until uniform PLGA/NHA and PLGA solutions were obtained. Then, the samples were added to the tube mold and then heated and cooled to obtain the composite porous scaffold of mesenchymal stem cells. 10 μl MSCs cell suspension was extracted with a microinjector, and the needle was injected from the inside of the scaffold, and the cell suspension was added outside the scaffold to ensure that there were composite cells inside and outside the scaffold. The subcutaneous tissue of the skin was cut along the medial side of the knee joint and the capsule of the switch segment was cut. The scaffold materials were filled into the osteochondral defect to observe the cartilage healing. The mechanical strength of 0.5% PLGA-MSCs composite porous scaffold was increased to 1.1 MPa, and the cell density was high. The repair of cartilage in rats was the best. The results showed that the porous scaffolds designed in this study have good compatibility and are beneficial to repair sports cartilage injury.

Fabrication and characterization of platelet-rich plasma scaffolds for tissue engineering applications

2017 ◽  
Vol 71 ◽  
pp. 372-380 ◽  
Author(s):  
Mahmoud Sadeghi-Ataabadi ◽  
Zohreh Mostafavi-pour ◽  
Zahra Vojdani ◽  
Mahsa Sani ◽  
Mona Latifi ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Platelet Rich Plasma ◽  
Engineering Applications ◽  
Fabrication And Characterization
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