Synthesis of Biogenic Silver Nanoparticles Using Caesalpinia digyna and Investigation of Their Antimicrobial Activity and In Vivo Biocompatibility

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Shakil Ahmed Polash ◽  
...  
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Iasmin R. Ferreira ◽  
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Ravi Naidu

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Priscila Jane Romano de Oliveira Gonçalves ◽  
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Rita de Cássia Ruiz ◽  
Marcelo Antonio Morgano ◽  
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Allan Radaic ◽  
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Wagner José Fávaro ◽  
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Gerson Nakasato ◽  
Marcelo Brocchi ◽  
Patricia S. Melo ◽  
Stephany C. Huber ◽  
...  

The silver nanoparticles production (~8 nm) byFusarium oxysporumwas evidenced by the presence of the plasmon absorption band. These particles were stable by several months due to protein capping originated by the biogenic process as observed by transmission electron microscopy (TEM). The cytotoxicity of silver nanoparticles was assayed on V79 fibroblast cell line and were evaluated by tetrazolium reduction and neutral red uptake giving an IC50of 22 μM. Silver nanoparticles impregnation in textile fabrics was made through the padding method and their impregnation was confirmed by SEM-EDS. The antimicrobial tests on the textile fabric were done with different bacteria. These fabrics showed antimicrobial activity against all the studied bacteria. The antimicrobial activity was maintained until the 30ª washes showing the high adhesion of these nanoparticles on the fabric fibers probably due to interaction between protein capping and fibers. Then, it was demonstrated an efficient method of stable silver nanoparticles production and their high adhesion on the textile fabrics.


Author(s):  
María Belén Estevez ◽  
María Laura Casaux ◽  
Martín Fraga ◽  
Ricardo Faccio ◽  
Silvana Alborés

Infectious diseases are one of the most important health problems worldwide, one of the main causes being the development of multi-resistant microorganisms. Likewise, the zoonotic potential of some pathogens and their ability to transfer resistance mechanisms, reduce the therapeutic options in both humans and animals. Salmonella enterica is an important pathogen that affects a wide range of animal species and humans, being Salmonella Typhimurium one of the most frequent serotypes affecting cattle, causing enteritis, diarrhea, and septicemia. The search for alternative therapeutic approaches has gained importance since the emergence of multidrug resistance to antibiotics and periodic outbreaks of salmonellosis. In this sense, the discovery of new drugs and the development of new strategies, such as the use of nanoparticles with antimicrobial activity, are very promising. The aim of this work was the extracellular production of biogenic silver nanoparticles using fungal extracts and the evaluation of their antimicrobial activity against resistant and multi-resistant Salmonella Typhimurium strains. We here demonstrated the potential of the biogenic nanoparticles as effective bacteriostatic and bactericidal agents for use in biomedical applications. In addition, Confocal Raman Microscopy and Atomic Force Microscopy were used to advance the understanding of the antimicrobial mechanism of biogenic nanoparticles against these pathogenic strains, the results of which suggested that the nanoparticles produced damage in several bacterial cell structures.


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