scholarly journals Three-Dimensional Circular Surface Curvature of a Spherule-Based Electrode for Selective Signaling and Dynamic Mobility of Norepinephrine in Living Cells

2020 ◽  
Vol 3 (12) ◽  
pp. 8496-8506
Author(s):  
Mohammed Y. Emran ◽  
Mohamed A. Shenashen ◽  
Sherif A. El-Safty ◽  
Mahmoud M. Selim ◽  
Takashi Minowa ◽  
...  
Keyword(s):  
Three Dimensional ◽  
Living Cells ◽  
Surface Curvature ◽  
Dynamic Mobility ◽  
Circular Surface

Autonomous Operation of 3D DNA Walkers in Living Cells for MicroRNA Imaging

Nanoscale ◽  
2021 ◽  
Author(s):  
Hui Hu ◽  
Fu Zhou ◽  
Baojuan Wang ◽  
Xin Chang ◽  
Tianyue Dai ◽  
...  
Keyword(s):  
Signal Transduction ◽  
Three Dimensional ◽  
Living Cells ◽  
Autonomous Operation ◽  
Dna Walkers

Three dimensional (3D) DNA walkers possesses the potential as ideal candidates for signal transduction and amplification in bioassays. However, intracellularly autonomous operation of 3D DNA walkers is still limitedly implemented...

scholarly journals 2P-022 Investigating protein three-dimensional structures inside living cells by in-cell NMR spectroscopy(The 46th Annual Meeting of the Biophysical Society of Japan)

2008 ◽  
Vol 48 (supplement) ◽  
pp. S78
Author(s):  
Junpei Hamatsu ◽  
Daisuke Sakakibara ◽  
Atsuko Sasaki ◽  
Teppei Ikeya ◽  
Masaki Mishima ◽  
...  
Keyword(s):  
Nmr Spectroscopy ◽  
Annual Meeting ◽  
Three Dimensional ◽  
Living Cells ◽  
Biophysical Society

New method of holographic three-dimensional tracking of living cells exploiting their morphological properties

2013 ◽  
Author(s):  
Pasquale Memmolo ◽  
Maria Iannone ◽  
Maurizio Ventre ◽  
Paolo Antonio Netti ◽  
Andrea Finizio ◽  
...  
Keyword(s):  
Three Dimensional ◽  
Living Cells ◽  
New Method ◽  
Morphological Properties

scholarly journals Developmental enhancers and chromosome topology

Science ◽  
2018 ◽  
Vol 361 (6409) ◽  
pp. 1341-1345 ◽  
Author(s):  
Eileen E. M. Furlong ◽  
Michael Levine
Keyword(s):  
Three Dimensional ◽  
Cell Types ◽  
Living Cells ◽  
Specific Cell ◽  
Transcriptional Dynamics ◽  
Spatiotemporal Expression ◽  
Chromosome Topology ◽  
Classical Models ◽  
And Function ◽  
Target Promoters

Developmental enhancers mediate on/off patterns of gene expression in specific cell types at particular stages during metazoan embryogenesis. They typically integrate multiple signals and regulatory determinants to achieve precise spatiotemporal expression. Such enhancers can map quite far—one megabase or more—from the genes they regulate. How remote enhancers relay regulatory information to their target promoters is one of the central mysteries of genome organization and function. A variety of contrasting mechanisms have been proposed over the years, including enhancer tracking, linking, looping, and mobilization to transcription factories. We argue that extreme versions of these mechanisms cannot account for the transcriptional dynamics and precision seen in living cells, tissues, and embryos. We describe emerging evidence for dynamic three-dimensional hubs that combine different elements of the classical models.

A High Fidelity Quality Assessment of High Pressure Turbine Blades Using Surface Curvature and Gradient-Based Adjoint

2018 ◽  
Author(s):  
Akhil Mulloth ◽  
Gabriel Banks ◽  
Giulio Zamboni ◽  
Simon Bather
Keyword(s):  
Quality Assessment ◽  
Turbine Blades ◽  
Three Dimensional ◽  
Surface Curvature ◽  
Assessment Process ◽  
Screening Process ◽  
Profile Tolerance ◽  
Gradient Based ◽  
Parts Manufacturing ◽  
The Impact

Gas turbine performance is highly dependent on the quality of the manufactured parts. Manufacturing variations in the parts can significantly alter the performance, especially efficiency and thus SFC. The legacy process is to accept variations within predefined profile tolerance limits and a few other qualitative parameters, mostly at a few, key two-dimensional aerofoil sections. With the widespread use of White light scans and other similar three-dimensional scans, this has improved to include the three-dimensional profile. The future however may lie with performance based quality assessment of manufactured parts, combined with quantitative surface quality assessment to implement an intelligent screening process for the parts. The adjoint method, typically used for shape optimization is adapted to provide a prediction of the impact on performance due to manufacturing variations. The work presented outlines a three stage quality assessment process for manufactured parts, involving three-dimensional profile tolerance based screening, followed by a surface curvature based screening and finally an Adjoint based performance prediction.

scholarly journals Label-free prediction of three-dimensional fluorescence images from transmitted light microscopy

2018 ◽  
Author(s):  
Chawin Ounkomol ◽  
Sharmishtaa Seshamani ◽  
Mary M. Maleckar ◽  
Forrest Collman ◽  
Gregory R. Johnson
Keyword(s):  
Fluorescence Microscopy ◽  
Three Dimensional ◽  
Living Cells ◽  
Transmitted Light ◽  
Integrated Systems ◽  
Label Free ◽  
Subcellular Structure ◽  
Modern Biology ◽  
Transmitted Light Microscopy ◽  
Fluorescence Images

Understanding living cells as integrated systems, a challenge central to modern biology, is complicated by limitations of available imaging methods. While fluorescence microscopy can resolve subcellular structure in living cells, it is expensive, slow, and damaging to cells. Here, we present a label-free method for predicting 3D fluorescence directly from transmitted light images and demonstrate that it can be used to generate multi-structure, integrated images.

scholarly journals Super-Resolution Imaging by Dual Iterative Structured Illumination Microscopy

2021 ◽  
Author(s):  
Anna Loeschberger ◽  
Yauheni Novikau ◽  
Ralf Netz ◽  
Marie-Christin Spindler ◽  
Ricardo Benavente ◽  
...  
Keyword(s):  
Three Dimensional ◽  
Brain Slices ◽  
Super Resolution ◽  
Reconstruction Algorithm ◽  
Living Cells ◽  
Structured Illumination ◽  
Structured Illumination Microscopy ◽  
Spatiotemporal Resolution ◽  
Coated Pits ◽  
Resolution Imaging

Three-dimensional (3D) multicolor super-resolution imaging in the 50-100 nm range in fixed and living cells remains challenging. We extend the resolution of structured illumination microscopy (SIM) by an improved nonlinear iterative reconstruction algorithm that enables 3D multicolor imaging with improved spatiotemporal resolution at low illumination intensities. We demonstrate the performance of dual iterative SIM (diSIM) imaging cellular structures in fixed cells including synaptonemal complexes, clathrin coated pits and the actin cytoskeleton with lateral resolutions of 60-100 nm with standard fluorophores. Furthermore, we visualize dendritic spines in 70 micrometer thick brain slices with an axial resolution < 200 nm. Finally, we image dynamics of the endoplasmatic reticulum and microtubules in living cells with up to 255 frames/s.

Interaction of Transient Waves With a Circular Surface-Piercing Body

1995 ◽  
Vol 117 (3) ◽  
pp. 382-388 ◽  
Author(s):  
Xing Yu ◽  
Ronald W. Yeung
Keyword(s):  
Large Time ◽  
Fluid Motion ◽  
Three Dimensional ◽  
Transient Waves ◽  
Generalized Poisson ◽  
Poisson Solver ◽  
Potential Applications ◽  
Circular Surface ◽  
High Degree ◽  
Pseudo Spectral

A pseudo-spectral formulation for solving unsteady, three-dimensional fluid motion with a free surface in cylindrical coordinates is presented. An effective method for treating the Laplace equation, as a special application of a generalized Poisson solver, is developed. This approach is demonstrated by studying the evolution of transient surface waves near a vertical circular cylinder enclosed in open or closed domains. Results are observed to have a high degree of precision and spatial resolution even at large time. Potential applications of this method to other problems are discussed.

scholarly journals Transport and localization of exogenous myelin basic protein mRNA microinjected into oligodendrocytes.

1993 ◽  
Vol 123 (2) ◽  
pp. 431-441 ◽  
Author(s):  
K Ainger ◽  
D Avossa ◽  
F Morgan ◽  
S J Hill ◽  
C Barry ◽  
...  
Keyword(s):  
Three Dimensional ◽  
Living Cells ◽  
Branch Points ◽  
Rna Granules ◽  
Intracellular Movement ◽  
Directional Movement ◽  
Ionic Detergent ◽  
Mrna Granules

We have studied transport and localization of MBP mRNA in oligodendrocytes in culture by microinjecting labeled mRNA into living cells and analyzing the intracellular distribution of the injected RNA by confocal microscopy. Injected mRNA initially appears dispersed in the perikaryon. Within minutes, the RNA forms granules which, in the case of MBP mRNA, are transported down the processes to the periphery of the cell where the distribution again becomes dispersed. In situ hybridization shows that endogenous MBP mRNA in oligodendrocytes also appears as granules in the perikaryon and processes and dispersed in the peripheral membranes. The granules are not released by extraction with non-ionic detergent, indicating that they are associated with the cytoskeletal matrix. Three dimensional visualization indicates that MBP mRNA granules are often aligned in tracks along microtubules traversing the cytoplasm and processes. Several distinct patterns of granule movement are observed. Granules in the processes undergo sustained directional movement with a velocity of approximately 0.2 micron/s. Granules at branch points undergo oscillatory motion with a mean displacement of 0.1 micron/s. Granules in the periphery of the cell circulate randomly with a mean displacement of approximately 1 micron/s. The results are discussed in terms of a multi-step pathway for transport and localization of MBP mRNA in oligodendrocytes. This work represents the first characterization of intracellular movement of mRNA in living cells, and the first description of the role of RNA granules in transport and localization of mRNA in cells.

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