Self-Healable Porous Polyampholyte Hydrogels with Higher Water Content as Cell Culture Scaffolds for Tissue Engineering Applications

2020 ◽  
Vol 3 (8) ◽  
pp. 5446-5453 ◽  
Author(s):  
Jui-Hsiang Liu ◽  
Yi-Hua Hung ◽  
Kai-Ti Chang ◽  
Chun-Yu Kao ◽  
Yu-Ting Lin ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Cell Culture ◽  
Water Content ◽  
Engineering Applications

Platelet-Rich Plasma in Tissue Engineering: Hype and Hope

2018 ◽  
Vol 59 (3-4) ◽  
pp. 265-275 ◽  
Author(s):  
Siegmund Lang ◽  
Markus Loibl ◽  
Marietta Herrmann
Keyword(s):  
Tissue Engineering ◽  
Cell Culture ◽  
Growth Factors ◽  
Progenitor Cells ◽  
Platelet Rich Plasma ◽  
Engineering Applications ◽  
Preparation Methods ◽  
Platelet Suspension ◽  
A Cell ◽  
Promising Source

Background: Platelet-rich plasma (PRP) refers to an enriched platelet suspension in plasma. In addition to the clinical application of PRP in the context of various orthopedic diseases and beyond, PRP and platelet lysate (PL) have been in focus in the field of tissue engineering. In this review, we discuss the application of PRP as a cell culture supplement and as part of tissue engineering strategies, particularly emphasizing current hurdles and ambiguities regarding the efficacy of PRP in these approaches. Summary: As a putative autologous replacement for animal-derived supplements such as fetal calf serum (FCS), PRP has been applied as cell culture supplement for the expansion of stem and progenitor cells for tissue engineering applications and cell therapies. Attributed to the high content of growth factors in platelets, PRP has been shown to promote cell growth, which was mostly superior to standard cultures supplemented with FCS, while the differentiation capacity of progenitor cells seems not to be affected. However, it was also suggested that cultivation of cells with PRP significantly alters the protein expression profile in cells in comparison to FCS, indicating that the influence of PRP on cell behavior should be thoroughly investigated. Moreover, different PRP preparation methods and donor variations have to be considered for the use of PRP under good manufacturing practice conditions. PRP has been used for various tissue engineering applications in the context of bone, cartilage, skin, and soft tissue repair, where most studies were conducted in the field of bone tissue engineering. These approaches take either advantage of the release of chemoattractive, angiogenic, proliferative, and putatively pro-regenerative growth factors from PRP, and/or the hydrogel properties of activated PRP, making it suitable as a cell delivery vehicle. In many of these studies, PRP is combined with biomaterials, cells, and in some cases recombinant growth factors. Although the experimental design often does not allow conclusions on the pro-regenerative effect of PRP itself, most publications report beneficial effects if PRP is added to the tissue-engineered construct. Furthermore, it was demonstrated that the release of growth factors from PRP may be tailored and controlled when PRP is combined with materials able to capture growth factors. Key Messages: Platelet-derived preparations such as PRP and PL represent a promising source of autologous growth factors, which may be applied as cell culture supplement or to promote regeneration in tissue-engineered constructs. Furthermore, activated PRP is a promising candidate as an autologous cell carrier. However, the studies investigating PRP in these contexts often show conflicting results, which most likely can be attributed to the lack of standardized preparation methods, particularly with regard to the platelet content and donor variation of PRP. Ultimately, the use of PRP has to be tailored for the individual application.

A Study on Bioscaffolds of Polysialic Acid and β-Glucan for Cell Culture and Tissue Engineering Applications

2010 ◽  
pp. 567-572
Author(s):  
Yvonne Stark ◽  
Stephanie Bruns ◽  
Bastian Rode ◽  
Silke Berski ◽  
Gerald Dräger ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Cell Culture ◽  
Polysialic Acid ◽  
Engineering Applications

Nanofibrous Substrate For Tissue Engineering Applications—A Review

2021 ◽  
Vol 8 (6) ◽  
pp. 13-21
Author(s):  
Odia Osemwegie ◽  
Lihua Lou ◽  
Ernest Smith ◽  
Seshadri Ramkumar
Keyword(s):  
Drug Delivery ◽  
Tissue Engineering ◽  
Cell Culture ◽  
Tissue Regeneration ◽  
Biomedical Applications ◽  
High Volume ◽  
Clinical Applications ◽  
Critical Factors ◽  
Engineering Applications

Nanofiber substrates have been used for various biomedical applications, including tissue regeneration, drug delivery, and in-vitro cell culture. However, despite the high volume of studies in this field, current clinical applications remain minimal. Innovations for their applications continuously generate exciting prospects. In this review, we discuss some of these novel innovations and identify critical factors to consider before their adoption for biomedical applications.

Effects of 3D-bioplotted polycaprolactone scaffold geometry on human adipose-derived stem cell viability and proliferation

2017 ◽  
Vol 23 (3) ◽  
pp. 534-542 ◽  
Author(s):  
Saahil V. Mehendale ◽  
Liliana F. Mellor ◽  
Michael A. Taylor ◽  
Elizabeth G. Loboa ◽  
Rohan A. Shirwaiker
Keyword(s):  
Tissue Engineering ◽  
Cell Culture ◽  
Stem Cell ◽  
Pore Size ◽  
Compression Modulus ◽  
Engineering Applications ◽  
Content Type ◽  
Musculoskeletal Tissue ◽  
Adipose Derived Stem Cell

Purpose This study aims to investigate the effect of three-dimensional (3D)- bioplotted polycaprolactone (PCL) scaffold geometry on the biological and mechanical characteristics of human adipose-derived stem cell (hASC) seeded constructs. Design/methodology/approach Four 3D-bioplotted scaffold disc designs (Ø14.5 × 2 mm) with two levels of strand–pore feature sizes and two strand laydown patterns (0°/90° or 0°/120°/240°) were evaluated for hASC viability, proliferation and construct compressive stiffness after 14 days of in vitro cell culture. Findings Scaffolds with the highest porosity (smaller strand–pore size in 0°/120°/240°) yielded the highest hASC proliferation and viability. Further testing of this design in a 6-mm thick configuration showed that cells were able to penetrate and proliferate throughout the scaffold thickness. The design with the lowest porosity (larger strand–pore size in 0°/90°) had the highest compression modulus after 14 days of culture, but resulted in the lowest hASC viability. The strand laydown pattern by itself did not influence the compression modulus of scaffolds. The 14-day cell culture also did not cause significant changes in compressive properties in any of the four designs. Originality/value hASC hold great potential for musculoskeletal tissue engineering applications because of their relative ease of harvest, abundance and differentiation abilities. This study reports on the effects of 3D-bioplotted scaffold geometry on mechanical and biological characteristics of hASC-seeded PCL constructs. The results provide the basis for future studies which will use this optimal scaffold design to develop constructs for hASC-based osteochondral tissue engineering applications.

Design and validation of a dynamic cell-culture system for bone biology research and exogenous tissue-engineering applications

2013 ◽  
Vol 10 (10) ◽  
pp. E327-E336 ◽  
Author(s):  
Alexander C. Allori ◽  
Edward H. Davidson ◽  
Derek D. Reformat ◽  
Alexander M. Sailon ◽  
James Freeman ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Cell Culture ◽  
Culture System ◽  
Cell Culture System ◽  
Bone Biology ◽  
Engineering Applications ◽  
Dynamic Cell ◽  
Biology Research
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