Paper-Based Cell Culture: Paving the Pathway for Liver Tissue Model Development on a Cellulose Paper Chip

2020 ◽  
Vol 3 (7) ◽  
pp. 3956-3974
Author(s):  
Tarun Agarwal ◽  
Mimi R. Borrelli ◽  
Pooyan Makvandi ◽  
Milad Ashrafizadeh ◽  
Tapas Kumar Maiti
Keyword(s):  
Cell Culture ◽  
Liver Tissue ◽  
Model Development ◽  
Tissue Model ◽  
Cellulose Paper ◽  
Paper Chip

scholarly journals Effects of Shiga Toxin Type 2 on a Bioengineered Three-Dimensional Model of Human Renal Tissue

2014 ◽  
Vol 83 (1) ◽  
pp. 28-38 ◽  
Author(s):  
Teresa M. DesRochers ◽  
Erica Palma Kimmerling ◽  
Dakshina M. Jandhyala ◽  
Wassim El-Jouni ◽  
Jing Zhou ◽  
...  
Keyword(s):  
Cell Culture ◽  
Renal Failure ◽  
Shiga Toxin ◽  
Three Dimensional ◽  
Kidney Injury ◽  
Renal Tissue ◽  
Three Dimensional Model ◽  
Drug Induced ◽  
Tissue Model

Shiga toxins (Stx) are a family of cytotoxic proteins that can cause hemolytic-uremic syndrome (HUS), a thrombotic microangiopathy, following infections by Shiga toxin-producingEscherichia coli(STEC). Renal failure is a key feature of HUS and a major cause of childhood renal failure worldwide. There are currently no specific therapies for STEC-associated HUS, and the mechanism of Stx-induced renal injury is not well understood primarily due to a lack of fully representative animal models and an inability to monitor disease progression on a molecular or cellular level in humans at early stages. Three-dimensional (3D) tissue models have been shown to be morein vivo-like in their phenotype and physiology than 2D cultures for numerous disease models, including cancer and polycystic kidney disease. It is unknown whether exposure of a 3D renal tissue model to Stx will yield a morein vivo-like response than 2D cell culture. In this study, we characterized Stx2-mediated cytotoxicity in a bioengineered 3D human renal tissue model previously shown to be a predictor of drug-induced nephrotoxicity and compared its response to Stx2 exposure in 2D cell culture. Our results demonstrate that although many mechanistic aspects of cytotoxicity were similar between 3D and 2D, treatment of the 3D tissues with Stx resulted in an elevated secretion of the kidney injury marker 1 (Kim-1) and the cytokine interleukin-8 compared to the 2D cell cultures. This study represents the first application of 3D tissues for the study of Stx-mediated kidney injury.

In Vitro Liver Tissue Model Established from Transgenic Mice: Role of HIF-1alpha on Hypoxic Gene Expression

2006 ◽  
Vol 12 (11) ◽  
pp. 3135-3147 ◽  
Author(s):  
Jared W. Allen ◽  
Salman R. Khetani ◽  
Randall S. Johnson ◽  
Sangeeta N. Bhatia
Keyword(s):  
Gene Expression ◽  
Transgenic Mice ◽  
Liver Tissue ◽  
Tissue Model

A Systematic Approach for Scale-Down Model Development and Characterization of Commercial Cell Culture Processes

2006 ◽  
Vol 22 (3) ◽  
pp. 696-703 ◽  
Author(s):  
F. Li ◽  
Y. Hashimura ◽  
R. Pendleton ◽  
J. Harms ◽  
E. Collins ◽  
...  
Keyword(s):  
Cell Culture ◽  
Systematic Approach ◽  
Model Development ◽  
Scale Down

Model Development and Analysis of Mammalian Cell Culture Systems

2011 ◽  
pp. 403-439
Author(s):  
Alexandros Kiparissides ◽  
Michalis Koutinas ◽  
Efstratios N. Pistikopoulos ◽  
Athanasios Mantalaris
Keyword(s):  
Cell Culture ◽  
Mammalian Cell ◽  
Mammalian Cell Culture ◽  
Model Development ◽  
Culture Systems ◽  
Cell Culture Systems

Concave microwell based size-controllable hepatosphere as a three-dimensional liver tissue model

Biomaterials ◽  
2011 ◽  
Vol 32 (32) ◽  
pp. 8087-8096 ◽  
Author(s):  
Sau Fung Wong ◽  
Da Yoon No ◽  
Yoon Young Choi ◽  
Dong Sik Kim ◽  
Bong Geun Chung ◽  
...  
Keyword(s):  
Liver Tissue ◽  
Three Dimensional ◽  
Tissue Model

Model Development and Analysis of Mammalian Cell Culture Systems

2014 ◽  
pp. 403-439
Author(s):  
Alexandros Kiparissides ◽  
Michalis Koutinas ◽  
Efstratios N. Pistikopoulos ◽  
Athanasios Mantalaris
Keyword(s):  
Cell Culture ◽  
Mammalian Cell ◽  
Mammalian Cell Culture ◽  
Model Development ◽  
Culture Systems ◽  
Cell Culture Systems
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