Interfacial Liquid–Liquid Phase Separation-Driven Polymerization-Induced Electrostatic Self-Assembly

2021 ◽  
Author(s):  
Ye Wang ◽  
Chao Li ◽  
Lei Ma ◽  
Xiyu Wang ◽  
Kai Wang ◽  
...  
Keyword(s):  
Phase Separation ◽  
Liquid Phase ◽  
Self Assembly ◽  
Liquid Phase Separation ◽  
Liquid Liquid Phase Separation

scholarly journals Spider silk self-assembly via modular liquid-liquid phase separation and nanofibrillation

2020 ◽  
Vol 6 (45) ◽  
pp. eabb6030
Author(s):  
Ali D. Malay ◽  
Takehiro Suzuki ◽  
Takuya Katashima ◽  
Nobuaki Kono ◽  
Kazuharu Arakawa ◽  
...  
Keyword(s):  
Phase Separation ◽  
Liquid Phase ◽  
Self Assembly ◽  
Spider Silk ◽  
Solid Phase ◽  
Silk Gland ◽  
Liquid Phase Separation ◽  
Hierarchical Organization ◽  
Amino Terminal ◽  
Liquid Liquid Phase Separation

Spider silk fiber rapidly assembles from spidroin protein in soluble state via an incompletely understood mechanism. Here, we present an integrated model for silk formation that incorporates the effects of multiple chemical and physical gradients on the different spidroin functional domains. Central to the process is liquid-liquid phase separation (LLPS) that occurs in response to multivalent anions such as phosphate, mediated by the carboxyl-terminal and repetitive domains. Acidification coupled with LLPS triggers the swift self-assembly of nanofibril networks, facilitated by dimerization of the amino-terminal domain, and leads to a liquid-to-solid phase transition. Mechanical stress applied to the fibril structures yields macroscopic fibers with hierarchical organization and enriched for β-sheet conformations. Studies using native silk gland material corroborate our findings on spidroin phase separation. Our results suggest an intriguing parallel between silk assembly and other LLPS-mediated mechanisms, such as found in intracellular membraneless organelles and protein aggregation disorders.

Polymerization-Induced Self-Assembly Promoted by Liquid–Liquid Phase Separation

2019 ◽  
Vol 8 (8) ◽  
pp. 943-946 ◽  
Author(s):  
Yi Ding ◽  
Qingqing Zhao ◽  
Lei Wang ◽  
Leilei Huang ◽  
Qizhou Liu ◽  
...  
Keyword(s):  
Phase Separation ◽  
Liquid Phase ◽  
Self Assembly ◽  
Liquid Phase Separation ◽  
Liquid Liquid Phase Separation

scholarly journals Liquid–liquid phase separation during amphiphilic self-assembly

2019 ◽  
Vol 11 (4) ◽  
pp. 320-328 ◽  
Author(s):  
Alessandro Ianiro ◽  
Hanglong Wu ◽  
Mark M. J. van Rijt ◽  
M. Paula Vena ◽  
Arthur D. A. Keizer ◽  
...  
Keyword(s):  
Phase Separation ◽  
Liquid Phase ◽  
Self Assembly ◽  
Liquid Phase Separation ◽  
Liquid Liquid Phase Separation

scholarly journals Liquid-Liquid Phase Separation of Tau Driven by Hydrophobic Interaction Facilitates Fibrillization of Tau

2021 ◽  
Vol 433 (2) ◽  
pp. 166731
Author(s):  
Yanxian Lin ◽  
Yann Fichou ◽  
Andrew P. Longhini ◽  
Luana C. Llanes ◽  
Pengyi Yin ◽  
...  
Keyword(s):  
Phase Separation ◽  
Liquid Phase ◽  
Hydrophobic Interaction ◽  
Liquid Phase Separation ◽  
Liquid Liquid Phase Separation

scholarly journals Amyloid Aggregation under the Lens of Liquid–Liquid Phase Separation

2020 ◽  
pp. 368-378
Author(s):  
Yanting Xing ◽  
Aparna Nandakumar ◽  
Aleksandr Kakinen ◽  
Yunxiang Sun ◽  
Thomas P. Davis ◽  
...  
Keyword(s):  
Phase Separation ◽  
Liquid Phase ◽  
Liquid Phase Separation ◽  
Amyloid Aggregation ◽  
Liquid Liquid Phase Separation

scholarly journals Observation of liquid-liquid phase separation of ataxin-3 and quantitative evaluation of its concentration in a single droplet using Raman microscopy

2021 ◽  
Author(s):  
Kazuki Murakami ◽  
Shinji Kajimoto ◽  
Daiki Shibata ◽  
Kunisato Kuroi ◽  
Fumihiko Fujii ◽  
...  
Keyword(s):  
Phase Separation ◽  
Liquid Phase ◽  
Neurodegenerative Diseases ◽  
Protein Aggregation ◽  
Quantitative Evaluation ◽  
Raman Microscopy ◽  
Liquid Phase Separation ◽  
Biological Processes ◽  
Single Droplet ◽  
Liquid Liquid Phase Separation

Liquid–liquid phase separation (LLPS) plays an important role in a variety of biological processes and is also associated with protein aggregation in neurodegenerative diseases. Quantification of LLPS is necessary to...

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