Interactome Analysis of the NS1 Protein Encoded by Influenza A H1N1 Virus Reveals a Positive Regulatory Role of Host Protein PRP19 in Viral Replication

2016 ◽  
Vol 15 (5) ◽  
pp. 1639-1648 ◽  
Author(s):  
Rei-Lin Kuo ◽  
Zong-Hua Li ◽  
Li-Hsin Li ◽  
Kuo-Ming Lee ◽  
Ee-Hong Tam ◽  
...  
Keyword(s):  
Viral Replication ◽  
Influenza A ◽  
H1n1 Virus ◽  
Host Protein ◽  
Regulatory Role ◽  
Ns1 Protein ◽  
Influenza A H1n1

scholarly journals Origins of the new influenza A(H1N1) virus: time to take action

2009 ◽  
Vol 14 (22) ◽  
Author(s):  
G M Nava ◽  
M S Attene-Ramos ◽  
J K Ang ◽  
M Escorcia
Keyword(s):  
Influenza A ◽  
H1n1 Virus ◽  
Phylogenetic Analyses ◽  
Protein Sequences ◽  
Interspecies Transmission ◽  
Genetic Evolution ◽  
Protein Homology ◽  
Influenza A H1n1 ◽  
Insight Into

To gain insight into the possible origins of the 2009 outbreak of new influenza A(H1N1), we performed two independent analyses of genetic evolution of the new influenza A(H1N1) virus. Firstly, protein homology analyses of more than 400 sequences revealed that this virus most likely evolved from recent swine viruses. Secondly, phylogenetic analyses of 5,214 protein sequences of influenza A(H1N1) viruses (avian, swine and human) circulating in North America for the last two decades (from 1989 to 2009) indicated that the new influenza A(H1N1) virus possesses a distinctive evolutionary trait (genetic distinctness). This appears to be a particular characteristic in pig-human interspecies transmission of influenza A. Thus these analyses contribute to the evidence of the role of pig populations as “mixing vessels” for influenza A(H1N1) viruses.

The PDZ-binding motif of the avian NS1 protein affects transmission of the 2009 influenza A(H1N1) virus

2014 ◽  
Vol 449 (1) ◽  
pp. 19-25 ◽  
Author(s):  
Jin Il Kim ◽  
Min-Woong Hwang ◽  
Ilseob Lee ◽  
Sehee Park ◽  
Sangmoo Lee ◽  
...  
Keyword(s):  
Influenza A ◽  
H1n1 Virus ◽  
Binding Motif ◽  
Ns1 Protein ◽  
Influenza A H1n1

In vitro Inhibitory Effects of Combinations of anti-Influenza Agents

1995 ◽  
Vol 6 (2) ◽  
pp. 109-113 ◽  
Author(s):  
L. K. Madren ◽  
C. Shipman ◽  
F. G. Hayden
Keyword(s):  
Viral Replication ◽  
Influenza A ◽  
H1n1 Virus ◽  
Mdck Cells ◽  
Clinical Isolates ◽  
H1n1 Subtype ◽  
Additive Effects ◽  
Inhibitory Effects ◽  
Influenza A H1n1

To assess the possible interactions among candidate anti-influenza agents, dual combinations of rimantadine, ribavirin, 2′-deoxy-2′-fluoroguanosine (2-FDG) and 4-guanidino-Neu5Ac2en (GG167) were tested against clinical isolates of influenza A H3N2 and H1N1 subtype viruses in MDCK cells by ELISA. Each of the dual combinations showed additive effects, except for the combination of 2-FDG and ribavirin which was synergistic against the influenza A (H1N1) virus. However, this combination also showed enhanced cytotoxicity. In this assay system, influenza agents with differing mechanisms of antiviral interaction interacted in an additive fashion with respect to inhibition of viral replication.

scholarly journals Role of tumor necrosis factor gene single nucleotide polymorphisms in the natural course of 2009 influenza A H1N1 virus infection

2012 ◽  
Vol 16 (3) ◽  
pp. e204-e208 ◽  
Author(s):  
Anastasia Antonopoulou ◽  
Fotini Baziaka ◽  
Thomas Tsaganos ◽  
Maria Raftogiannis ◽  
Pantelis Koutoukas ◽  
...  
Keyword(s):  
Influenza A ◽  
Tumor Necrosis ◽  
H1n1 Virus ◽  
Nucleotide Polymorphisms ◽  
Single Nucleotide ◽  
Factor Gene ◽  
Influenza A H1n1 ◽  
Necrosis Factor ◽  
H1n1 Virus Infection

Critical role of microRNAs in host and influenza A (H1N1) virus interactions

Life Sciences ◽  
2021 ◽  
pp. 119484
Author(s):  
Yuchong Wang ◽  
Xujun Zhang ◽  
Kefan Bi ◽  
Hongyan Diao
Keyword(s):  
Influenza A ◽  
H1n1 Virus ◽  
Critical Role ◽  
Influenza A H1n1 ◽  
Virus Interactions

scholarly journals Virulence Determinants of Avian H5N1 Influenza A Virus in Mammalian and Avian Hosts: Role of the C-Terminal ESEV Motif in the Viral NS1 Protein

2010 ◽  
Vol 84 (20) ◽  
pp. 10708-10718 ◽  
Author(s):  
Florian Zielecki ◽  
Ilia Semmler ◽  
Donata Kalthoff ◽  
Daniel Voss ◽  
Susanne Mauel ◽  
...  
Keyword(s):  
Influenza Virus ◽  
Avian Influenza ◽  
Viral Replication ◽  
Influenza A ◽  
Pdz Domain ◽  
Influenza Viruses ◽  
Dependent Manner ◽  
Ns1 Protein ◽  
H5n1 Influenza

ABSTRACT We assessed the prediction that access of the viral NS1 protein to cellular PDZ domain protein networks enhances the virulence of highly pathogenic avian influenza A viruses. The NS1 proteins of most avian influenza viruses bear the C-terminal ligand sequence Glu-Ser-Glu-Val (ESEV) for PDZ domains present in multiple host proteins, whereas no such motif is found in the NS1 homologues of seasonal human virus strains. Previous analysis showed that a C-terminal ESEV motif increases viral virulence when introduced into the NS1 protein of mouse-adapted H1N1 influenza virus. To examine the role of the PDZ domain ligand motif in avian influenza virus virulence, we generated three recombinants, derived from the prototypic H5N1 influenza A/Vietnam/1203/04 virus, expressing NS1 proteins that either have the C-terminal ESEV motif or the human influenza virus RSKV consensus or bear a natural truncation of this motif, respectively. Cell biological analyses showed strong control of NS1 nuclear migration in infected mammalian and avian cells, with only minor differences between the three variants. The ESEV sequence attenuated viral replication on cultured human, murine, and duck cells but not on chicken fibroblasts. However, all three viruses caused highly lethal infections in mice and chickens, with little difference in viral titers in organs, mean lethal dose, or intravenous pathogenicity index. These findings demonstrate that a PDZ domain ligand sequence in NS1 contributes little to the virulence of H5N1 viruses in these hosts, and they indicate that this motif modulates viral replication in a strain- and host-dependent manner.

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