scholarly journals In-Depth N-Glycosylation Reveals Species-Specific Modifications and Functions of the Royal Jelly Protein from Western (Apis mellifera) and Eastern Honeybees (Apis cerana)

2015 ◽  
Vol 14 (12) ◽  
pp. 5327-5340 ◽  
Author(s):  
Mao Feng ◽  
Yu Fang ◽  
Bin Han ◽  
Xiang Xu ◽  
Pei Fan ◽  
...  
Keyword(s):  
Apis Mellifera ◽  
Royal Jelly ◽  
Apis Cerana ◽  
Species Specific ◽  
Royal Jelly Protein

scholarly journals Authentication of Apis cerana Honey and Apis mellifera Honey Based on Major Royal Jelly Protein 2 Gene

Molecules ◽  
2019 ◽  
Vol 24 (2) ◽  
pp. 289 ◽  
Author(s):  
Yan-Zheng Zhang ◽  
Shuai Wang ◽  
Yi-Fan Chen ◽  
Yu-Qi Wu ◽  
Jing Tian ◽  
...  
Keyword(s):  
Apis Mellifera ◽  
Royal Jelly ◽  
Gene Selection ◽  
Apis Cerana ◽  
Melt Curve Analysis ◽  
Pcr Product ◽  
Authentication System ◽  
Rapid Discrimination ◽  
Species Specific ◽  
Royal Jelly Protein

In Asia, honey is mainly produced by Apis mellifera and Apis cerana. However, the price of A. cerana honey is usually much higher than A. mellifera honey. Seeing considerable profits, some dishonest companies and beekeepers mislabel A. mellifera honey as A. cerana honey or incorporate A. mellifera honey into A. cerana honey. In the present study, we developed methods to discriminate A. cerana honey from A. mellifera honey based on the MRJP2 (major royal jelly protein 2) gene. Two pairs of species-specific primers were designed. The amplification products of A. cerana and A. mellifera were 212 and 560 bp, respectively. As little as one percent incorporation of A. mellifera honey in the mixture can be detected by duplex PCR. Additionally, another method based on the melt curve analysis using the same primers was also developed, allowing a rapid discrimination of real-time PCR product of different species. Our study shows that the entomological authentication of honey samples can be identified by nuclear genes other than mitochondrial genes and this extends the possibility of gene selection in identification. The authentication system we proposed could be a useful tool for discriminating A. cerana honey from A. mellifera honey.

New haplotypes of Apis cerana in Indonesia: identification from mitochondrial and major royal jelly protein 2 genes

2021 ◽  
Author(s):  
Rika Raffiudin ◽  
Nurul Insani Shullia ◽  
Adelia Ulfie Damayanti ◽  
Dian Tri Wahyudi ◽  
Tia Vina Febiriani ◽  
...  
Keyword(s):  
Royal Jelly ◽  
Apis Cerana ◽  
Royal Jelly Protein

scholarly journals Calcium effect and pH-dependence on self-association and structural stability of the Apis mellifera major royal jelly protein 1

Apidologie ◽  
2011 ◽  
Vol 42 (3) ◽  
pp. 252-269 ◽  
Author(s):  
Gabriel C. N. Cruz ◽  
Liudy Garcia ◽  
Adelson J. Silva ◽  
João A. R. G. Barbosa ◽  
Carlos A. O. Ricart ◽  
...  
Keyword(s):  
Apis Mellifera ◽  
Structural Stability ◽  
Royal Jelly ◽  
Ph Dependence ◽  
Calcium Effect ◽  
Self Association ◽  
Royal Jelly Protein

scholarly journals Structure of native glycolipoprotein filaments in honeybee royal jelly

2020 ◽  
Vol 11 (1) ◽  
Author(s):  
Simone Mattei ◽  
Arvid Ban ◽  
Armin Picenoni ◽  
Marc Leibundgut ◽  
Rudi Glockshuber ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Apis Mellifera ◽  
Larval Development ◽  
Royal Jelly ◽  
Structural Data ◽  
High Viscosity ◽  
Cryo Electron Microscopy ◽  
Ph Dependent ◽  
High Concentrations ◽  
Royal Jelly Protein

AbstractRoyal jelly (RJ) is produced by honeybees (Apis mellifera) as nutrition during larval development. The high viscosity of RJ originates from high concentrations of long lipoprotein filaments that include the glycosylated major royal jelly protein 1 (MRJP1), the small protein apisimin and insect lipids. Using cryo-electron microscopy we reveal the architecture and the composition of RJ filaments, in which the MRJP1 forms the outer shell of the assembly, surrounding stacked apisimin tetramers harbouring tightly packed lipids in the centre. The structural data rationalize the pH-dependent disassembly of RJ filaments in the gut of the larvae.

scholarly journals Changes in Alternative Splicing in Apis Mellifera Bees Fed Apis Cerana Royal Jelly

2014 ◽  
Vol 58 (2) ◽  
pp. 25-31 ◽  
Author(s):  
Yuan Yuan Shi ◽  
Zachary Y. Huang ◽  
Xiao Bo Wu ◽  
Zi Long Wang ◽  
Wei Yu Yan ◽  
...  
Keyword(s):  
Alternative Splicing ◽  
Apis Mellifera ◽  
Honey Bee ◽  
Royal Jelly ◽  
Intron Retention ◽  
Tricarboxylic Acid Cycle ◽  
Apis Cerana ◽  
Causative Factor ◽  
Caste Differentiation ◽  
Splice Sites

Abstract The Western honey bee (Apis mellifera) is a social insect characterized by caste differentiation in which the queen bee and worker bees display marked differences in morphology, behavior, reproduction, and longevity despite their identical genomes. The main causative factor in caste differentiation is the food fed to queen larvae, termed royal jelly (RJ). Alternative splicing (AS) is an important RNA-mediated post-transcriptional process in eukaryotes. Here we report AS changes in A. mellifera after being fed either A. mellifera RJ or A. cerana RJ. The results demonstrated that the RJ type affected 4 types of AS in adult A. mellifera: exon skipping, intron retention, alternative 5’ splice sites, and alternative 3’splice sites. After feeding with A. cerana RJ, AS occurred in many genes in adult A. mellifera that encode proteins involved in development, growth, the tricarboxylic acid cycle, and substance metabolism. This study provides the first evidence that heterospecific RJ can influence the AS of many genes related to honey bee development and growth.

Honeybee (Apis cerana) major royal jelly protein 4 exhibits antimicrobial activity

2019 ◽  
Vol 22 (1) ◽  
pp. 175-182 ◽  
Author(s):  
Bo Yeon Kim ◽  
Kwang Sik Lee ◽  
Boknam Jung ◽  
Yong Soo Choi ◽  
Hye Kyung Kim ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Royal Jelly ◽  
Apis Cerana ◽  
Royal Jelly Protein

Carbendazim exposure during the larval stage suppresses major royal jelly protein expression in nurse bees (Apis mellifera)

Chemosphere ◽  
2021 ◽  
Vol 266 ◽  
pp. 129011
Author(s):  
Kang Wang ◽  
Heng Chen ◽  
Zhe-Guang Lin ◽  
Qing-Sheng Niu ◽  
Zhi Wang ◽  
...  
Keyword(s):  
Apis Mellifera ◽  
Protein Expression ◽  
Larval Stage ◽  
Royal Jelly ◽  
Royal Jelly Protein
Sign in / Sign up

Export Citation Format

Share Document