scholarly journals OH-Radical Specific Addition to Glutathione S-Atom at the Air–Water Interface: Relevance to the Redox Balance of the Lung Epithelial Lining Fluid

2015 ◽  
Vol 6 (19) ◽  
pp. 3935-3943 ◽  
Author(s):  
Shinichi Enami ◽  
Michael R. Hoffmann ◽  
Agustín J. Colussi
Keyword(s):  
Redox Balance ◽  
Oh Radical ◽  
Water Interface ◽  
Epithelial Lining ◽  
Epithelial Lining Fluid ◽  
Lung Epithelial ◽  
Air Water Interface

scholarly journals Lipid phenotyping of lung epithelial lining fluid in healthy human volunteers

Metabolomics ◽  
2018 ◽  
Vol 14 (10) ◽  
Author(s):  
Joost Brandsma ◽  
◽  
Victoria M. Goss ◽  
Xian Yang ◽  
Per S. Bakke ◽  
...  
Keyword(s):  
Epithelial Lining ◽  
Healthy Human ◽  
Epithelial Lining Fluid ◽  
Human Volunteers ◽  
Lung Epithelial

Recombinant secretory leukoprotease inhibitor augments glutathione levels in lung epithelial lining fluid

1993 ◽  
Vol 75 (2) ◽  
pp. 825-832 ◽  
Author(s):  
A. Gillissen ◽  
P. Birrer ◽  
N. G. McElvaney ◽  
R. Buhl ◽  
C. Vogelmeier ◽  
...  
Keyword(s):  
Serine Proteases ◽  
Lung Diseases ◽  
Antioxidant Defenses ◽  
Epithelial Lining ◽  
Upper Airways ◽  
Epithelial Surface ◽  
Epithelial Lining Fluid ◽  
Lung Epithelial ◽  
The Status ◽  
Respiratory Epithelial

Secretory leukoprotease inhibitor (SLPI), a 12-kDa serine antiprotease, serves as the major inhibitor of neutrophil elastase (NE) on the epithelial surface of the upper airways. As a control for studies to evaluate the aerosol administration of recombinant SLPI (rSLPI) to augment the anti-NE defenses of the lung, the status of antioxidants in respiratory epithelial lining fluid (ELF) was evaluated. Unexpectedly, aerosol administration of rSLPI caused an elevation in ELF glutathione, a major component of the epithelial antioxidant screen; i.e., rSLPI may provide not only augmentation of anti-NE defenses but also antioxidant defenses. To evaluate this concept, rSLPI (100 mg) was aerosolized to sheep, and SLPI, glutathione, anti-NE capacity, and anti-H2O2 capacity were evaluated in respiratory ELF over a 30-h period. As expected, aerosolization of rSLPI increased ELF SLPI levels and anti-NE capacity. Strikingly, postaerosol levels of glutathione in ELF were also increased (5-fold 24 h after aerosol), with a concomitant increase in ELF anti-H2O2 capacity; i.e., the rSLPI augmented the antioxidant screen of ELF. This suggests that rSLPI may be particularly well suited for therapy in lung diseases characterized by excess of both serine proteases and oxidants on the respiratory epithelial surface.

Role of transferrin and ceruloplasmin in antioxidant activity of lung epithelial lining fluid

1988 ◽  
Vol 64 (5) ◽  
pp. 2092-2099 ◽  
Author(s):  
E. R. Pacht ◽  
W. B. Davis
Keyword(s):  
Lipid Peroxidation ◽  
Antioxidant Activity ◽  
Serum Proteins ◽  
Antioxidant Properties ◽  
Epithelial Lining ◽  
Epithelial Lining Fluid ◽  
Lung Epithelial ◽  
Ferroxidase Activity

Lung epithelial lining fluid (ELF) is a thin layer of plasma ultrafiltrate and locally secreted substances that may provide antioxidant protection and serve as a "front-line" defense for the lower respiratory tract epithelium. To characterize the antioxidant properties of ELF, young, healthy, nonsmoking volunteers underwent bronchoalveolar lavage with determination of ELF volumes and ELF proteins. ELF (greater than 0.4 ml) is a potent inhibitor of lipid peroxidation as measured by malondialdehyde (MDA) production in an in vitro iron-dependent assay system. Two serum proteins, transferrin and ceruloplasmin, were quantitated in ELF and found to be potent inhibitors of lipid peroxidation. Other ELF components, including vitamin E, vitamin C, and albumin, did not function as antioxidants in this system. Several experimental observations suggest that ELF transferrin was more important than ceruloplasmin in inhibiting lipid peroxidation: 1) ELF concentrations of transferrin were 20-fold higher than those for ceruloplasmin; 2) ELF antioxidant activity was abolished by preincubation with Fe3+; 3) ELF antioxidant activity was minimally affected by sodium azide, which is known to inhibit ceruloplasmin ferroxidase activity; and 4) ELF ceruloplasmin ferroxidase activity was virtually nondetectable. ELF possesses a significant antioxidant activity that may be important in vivo in protecting the lung from oxidant injury.

scholarly journals Pooled population pharmacokinetic model of imipenem in plasma and the lung epithelial lining fluid

2016 ◽  
Vol 81 (6) ◽  
pp. 1113-1123 ◽  
Author(s):  
J. G. Coen van Hasselt ◽  
Matthew L. Rizk ◽  
Mallika Lala ◽  
Cynthia Chavez-Eng ◽  
Sandra A. G. Visser ◽  
...  
Keyword(s):  
Population Pharmacokinetic Model ◽  
Pharmacokinetic Model ◽  
Population Pharmacokinetic ◽  
Epithelial Lining ◽  
Epithelial Lining Fluid ◽  
Lung Epithelial

Proteomic Analysis of Undiluted Lung Epithelial Lining Fluid

CHEST Journal ◽  
2008 ◽  
Vol 134 (2) ◽  
pp. 338-345 ◽  
Author(s):  
Eric Kipnis ◽  
Kirk Hansen ◽  
Teiji Sawa ◽  
Kiyoshi Moriyama ◽  
Ashley Zurawel ◽  
...  
Keyword(s):  
Proteomic Analysis ◽  
Epithelial Lining ◽  
Epithelial Lining Fluid ◽  
Lung Epithelial

scholarly journals Structure-Based Prediction of Anti-infective Drug Concentrations in the Human Lung Epithelial Lining Fluid

2015 ◽  
Vol 33 (4) ◽  
pp. 856-867 ◽  
Author(s):  
Pyry A. J. Välitalo ◽  
Koen Griffioen ◽  
Matthew L. Rizk ◽  
Sandra A. G. Visser ◽  
Meindert Danhof ◽  
...  
Keyword(s):  
Human Lung ◽  
Epithelial Lining ◽  
Epithelial Lining Fluid ◽  
Drug Concentrations ◽  
Lung Epithelial
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