Photophysical and Thermodynamic Properties of Ag29(BDT)12(TPP)x (x = 0–4) Clusters in Secondary Ligand Binding–Dissociation Equilibria Unraveled by Photoluminescence Analysis

2020 ◽  
Vol 124 (10) ◽  
pp. 5880-5886 ◽  
Author(s):  
Yoshiki Niihori ◽  
Naoya Takahashi ◽  
Masaaki Mitsui
Keyword(s):  
Thermodynamic Properties ◽  
Ligand Binding ◽  
Secondary Ligand

scholarly journals Impact of protein–ligand solvation and desolvation on transition state thermodynamic properties of adenosine A2A ligand binding kinetics

2017 ◽  
Vol 5 (1) ◽  
Author(s):  
Giuseppe Deganutti ◽  
Andrei Zhukov ◽  
Francesca Deflorian ◽  
Stephanie Federico ◽  
Giampiero Spalluto ◽  
...  
Keyword(s):  
Thermodynamic Properties ◽  
Ligand Binding ◽  
Transition State ◽  
Binding Kinetics ◽  
Adenosine A2a

scholarly journals Identification of Two Secondary Ligand Binding Sites in 14-3-3 Proteins Using Fragment Screening

Biochemistry ◽  
2017 ◽  
Vol 56 (30) ◽  
pp. 3972-3982 ◽  
Author(s):  
Eline Sijbesma ◽  
Lukasz Skora ◽  
Seppe Leysen ◽  
Luc Brunsveld ◽  
Uwe Koch ◽  
...  
Keyword(s):  
Ligand Binding ◽  
Binding Sites ◽  
Fragment Screening ◽  
Ligand Binding Sites ◽  
Secondary Ligand

Thermodynamic properties of ligand binding by monoclonal anti-fluorescyl antibodies

Biochemistry ◽  
1986 ◽  
Vol 25 (16) ◽  
pp. 4602-4609 ◽  
Author(s):  
James N. Herron ◽  
David M. Kranz ◽  
David M. Jameson ◽  
Edward W. Voss
Keyword(s):  
Thermodynamic Properties ◽  
Ligand Binding

Models of Thermodynamic Properties of Prominences

1979 ◽  
Vol 44 ◽  
pp. 349-355
Author(s):  
R.W. Milkey
Keyword(s):  
Thermodynamic Properties ◽  
Mass Transport ◽  
Emission Spectrum ◽  
Thermodynamic Parameters ◽  
Working Group ◽  
Physical Processes ◽  
Theoretical Concepts ◽  
Group 3 ◽  
Observational Techniques

The focus of discussion in Working Group 3 was on the Thermodynamic Properties as determined spectroscopically, including the observational techniques and the theoretical modeling of physical processes responsible for the emission spectrum. Recent advances in observational techniques and theoretical concepts make this discussion particularly timely. It is wise to remember that the determination of thermodynamic parameters is not an end in itself and that these are interesting chiefly for what they can tell us about the energetics and mass transport in prominences.

CarR, a MarR-family regulator from Corynebacterium glutamicum, modulated antibiotic and aromatic compound resistance

2019 ◽  
Vol 476 (21) ◽  
pp. 3141-3159 ◽  
Author(s):  
Meiru Si ◽  
Can Chen ◽  
Zengfan Wei ◽  
Zhijin Gong ◽  
GuiZhi Li ◽  
...  
Keyword(s):  
Stress Response ◽  
Ligand Binding ◽  
Corynebacterium Glutamicum ◽  
Conformational Changes ◽  
Cysteine Oxidation ◽  
Transcriptional Regulatory ◽  
Ligand Free ◽  
Redox Sensing

Abstract MarR (multiple antibiotic resistance regulator) proteins are a family of transcriptional regulators that is prevalent in Corynebacterium glutamicum. Understanding the physiological and biochemical function of MarR homologs in C. glutamicum has focused on cysteine oxidation-based redox-sensing and substrate metabolism-involving regulators. In this study, we characterized the stress-related ligand-binding functions of the C. glutamicum MarR-type regulator CarR (C. glutamicum antibiotic-responding regulator). We demonstrate that CarR negatively regulates the expression of the carR (ncgl2886)–uspA (ncgl2887) operon and the adjacent, oppositely oriented gene ncgl2885, encoding the hypothetical deacylase DecE. We also show that CarR directly activates transcription of the ncgl2882–ncgl2884 operon, encoding the peptidoglycan synthesis operon (PSO) located upstream of carR in the opposite orientation. The addition of stress-associated ligands such as penicillin and streptomycin induced carR, uspA, decE, and PSO expression in vivo, as well as attenuated binding of CarR to operator DNA in vitro. Importantly, stress response-induced up-regulation of carR, uspA, and PSO gene expression correlated with cell resistance to β-lactam antibiotics and aromatic compounds. Six highly conserved residues in CarR were found to strongly influence its ligand binding and transcriptional regulatory properties. Collectively, the results indicate that the ligand binding of CarR induces its dissociation from the carR–uspA promoter to derepress carR and uspA transcription. Ligand-free CarR also activates PSO expression, which in turn contributes to C. glutamicum stress resistance. The outcomes indicate that the stress response mechanism of CarR in C. glutamicum occurs via ligand-induced conformational changes to the protein, not via cysteine oxidation-based thiol modifications.

Sign in / Sign up

Export Citation Format

Share Document