scholarly journals Insight into Size- and Metal-Dependent Activity and the Mechanism for Steam Methane Re-forming in Nanocatalysis

2020 ◽  
Vol 124 (4) ◽  
pp. 2501-2512
Author(s):  
Yalan Wang ◽  
Ling Xiao ◽  
Yanying Qi ◽  
Jia Yang ◽  
Yi-An Zhu ◽  
...  
Keyword(s):  
Steam Methane ◽  
Dependent Activity ◽  
Insight Into

scholarly journals Model and Algorithm for Dependent Activity Schedule Optimization Combining with BIM

2020 ◽  
Vol 2020 ◽  
pp. 1-14
Author(s):  
Guofeng Ma ◽  
Xiaoye Liu
Keyword(s):  
Monte Carlo Simulation ◽  
Communication Strategies ◽  
Dynamic Control ◽  
Project Duration ◽  
Schedule Optimization ◽  
Activity Schedule ◽  
Dependent Activity ◽  
Before And After ◽  
Reward Amount ◽  
Insight Into

The project duration can be shortened by overlapping construction activities. However, the continuous changing of the environment tends to cause problems such as rework and the failure of the overlapping plan. In order to solve these problems, communication strategies for the overlapping of dependent activities are first introduced and optimized from a revenue perspective. We first consider the different maturities of upstream activity before and after the overlapping, the downstream sensitivity which is decided by involving communication strategies, and the learning and error-correcting ability of workers. Then, the overlap and communication strategies are decided by calculating the maximum revenue using Monte Carlo simulation and MATLAB based on overlap cost, communication cost, rework cost, and reward amount. Finally, the algorithm and BIM are combined to provide a visual overlap plan and dynamic control platform framework. This research is valuable for practitioners as it provides a dynamic overlap plan which can maximize the revenue in changing the environment and ensure the duration of the project. This research also provides researchers a new insight into combining overlap problems and BIM technology.

scholarly journals Quantitative Proteome Profiling of a S-Nitrosoglutathione Reductase (GSNOR) Null Mutant Reveals a New Class of Enzymes Involved in Nitric Oxide Homeostasis in Plants

2021 ◽  
Vol 12 ◽  
Author(s):  
Patrick Treffon ◽  
Jacopo Rossi ◽  
Giuseppe Gabellini ◽  
Paolo Trost ◽  
Mirko Zaffagnini ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Oxidation Products ◽  
Null Mutant ◽  
Chlorophyll Metabolism ◽  
New Class ◽  
Reduction Activity ◽  
Dependent Component ◽  
Dependent Activity ◽  
Major Route ◽  
Insight Into

Nitric oxide (NO) is a short-lived radical gas that acts as a signaling molecule in all higher organisms, and that is involved in multiple plant processes, including germination, root growth, and fertility. Regulation of NO-levels is predominantly achieved by reaction of oxidation products of NO with glutathione to form S-nitrosoglutathione (GSNO), the principal bioactive form of NO. The enzyme S-nitrosoglutathione reductase (GSNOR) is a major route of NADH-dependent GSNO catabolism and is critical to NO homeostasis. Here, we performed a proteomic analysis examining changes in the total leaf proteome of an Arabidopsis thaliana GSNOR null mutant (hot5-2/gsnor1-3). Significant increases or decreases in proteins associated with chlorophyll metabolism and with redox and stress metabolism provide insight into phenotypes observed in hot5-2/gsnor1-3 plants. Importantly, we identified a significant increase in proteins that belong to the aldo-keto reductase (AKR) protein superfamily, AKR4C8 and 9. Because specific AKRs have been linked to NO metabolism in mammals, we expressed and purified A. thaliana AKR4C8 and 9 and close homologs AKR4C10 and 11 and determined that they have NADPH-dependent activity in GSNO and S-nitroso-coenzyme A (SNO-CoA) reduction. Further, we found an increase of NADPH-dependent GSNO reduction activity in hot5-2/gsnor1-3 mutant plants. These data uncover a new, NADPH-dependent component of NO metabolism that may be integrated with NADH-dependent GSNOR activity to control NO homeostasis in plants.

scholarly journals The Structure- and Metal-dependent Activity ofEscherichia coliPgaB Provides Insight into the Partial De-N-acetylation of Poly-β-1,6-N-acetyl-d-glucosamine

2012 ◽  
Vol 287 (37) ◽  
pp. 31126-31137 ◽  
Author(s):  
Dustin J. Little ◽  
Joanna Poloczek ◽  
John C. Whitney ◽  
Howard Robinson ◽  
Mark Nitz ◽  
...  
Keyword(s):  
Dependent Activity ◽  
Insight Into

scholarly journals Electrophoretic Characterization of Venom Proteins of Montivipera xanthina (Gray, 1849) (Ophidia: Viperidae)

2017 ◽  
Author(s):  
Hüseyin Arıkan ◽  
Nurşen Alpagut Keskin ◽  
Kerim Çiçek
Keyword(s):  
Serine Proteases ◽  
Polyacrylamide Gel Electrophoresis ◽  
Sodium Dodecyl ◽  
Sds Page ◽  
Dependent Activity ◽  
Fibrinogenolytic Activity ◽  
Identification And Characterization ◽  
Insight Into ◽  
Venom Proteins

In this study, with the aim of evaluating coagulant activities in the venom of M. xanthina, we analyzed venom proteins, digestion patterns of fibrinogen chains with venom and the effects of protease inhibitors on M. xanthina venom proteases using Tricine-sodium dodecyl sulfate polyacrylamide gel electrophoresis. Venom samples were obtained from four adult specimens of Montivipera xanthina collected in Gümüldür (Izmir, Turkey). SDS-PAGE analysis demonstrated that 17 protein bands in the range of 20–250 kDa were present. The specific digestion patterns of fibrinogen chains revealed that M. xanthina venom possesses fibrinogenolytic enzymes which could be included in coagulation processes during envenomation Fibrinogenolytic activity directed exclusively towards the Aa-chain with a time-dependent activity towards Bb-chains suggests the presence of both metalloproteinases and serine proteases in M. xanthine venom. In the present study, the occurrence and inhibition of fibrinogenolytic activity of M. xanthina venom were clearly observed. For further analysis, the isolation, identification, and characterization of individual venom components will provide insight into their function and biological roles.

scholarly journals Human pancreatic islet 3D chromatin architecture provides insights into the genetics of type 2 diabetes

2018 ◽  
Author(s):  
Irene Miguel-Escalada ◽  
Silvia Bonàs-Guarch ◽  
Inês Cebola ◽  
Ponsa-Cobas Joan ◽  
Julen Mendieta-Esteban ◽  
...  
Keyword(s):  
Type 2 Diabetes ◽  
Molecular Mechanisms ◽  
Target Genes ◽  
Genetic Studies ◽  
Chromatin Architecture ◽  
Genome Maps ◽  
3D Space ◽  
Dependent Activity ◽  
Insight Into

AbstractGenetic studies promise to provide insight into the molecular mechanisms underlying type 2 diabetes (T2D). Variants associated with T2D are often located in tissue-specific enhancer regions (enhancer clusters, stretch enhancers or super-enhancers). So far, such domains have been defined through clustering of enhancers in linear genome maps rather than in 3D-space. Furthermore, their target genes are generally unknown. We have now created promoter capture Hi-C maps in human pancreatic islets. This linked diabetes-associated enhancers with their target genes, often located hundreds of kilobases away. It further revealed sets of islet enhancers, super-enhancers and active promoters that form 3D higher-order hubs, some of which show coordinated glucose-dependent activity. Hub genetic variants impact the heritability of insulin secretion, and help identify individuals in whom genetic variation of islet function is important for T2D. Human islet 3D chromatin architecture thus provides a framework for interpretation of T2D GWAS signals.

scholarly journals Thiouracil-Forming Bacteria Identified and Characterized upon PorcineIn VitroDigestion of Brassicaceae Feed

2014 ◽  
Vol 80 (23) ◽  
pp. 7433-7442 ◽  
Author(s):  
Julie A. L. Kiebooms ◽  
Jella Wauters ◽  
Julie Vanden Bussche ◽  
Kurt Houf ◽  
Paul De Vos ◽  
...  
Keyword(s):  
Lactobacillus Reuteri ◽  
Bacterial Species ◽  
Underlying Mechanism ◽  
Molecular Techniques ◽  
Microbial Conversion ◽  
Content Type ◽  
Dependent Activity ◽  
Liquid Chromatography Tandem Mass ◽  
Insight Into

ABSTRACTIn recent years, the frequent detection of the banned thyreostat thiouracil (TU) in livestock urine has been related to endogenous TU formation following digestion of glucosinolate-rich Brassicaceae crops. Recently, it was demonstrated that, uponin vitrodigestion of Brassicaceae, fecal bacteria induce TU detection in livestock (porcine livestock > bovines). Therefore, the present study was intended to isolate and identify bacteria involved in this intestinal TU formation upon Brassicaceae digestion and to gain more insight into the underlying mechanism in porcine livestock. Twenty porcine fecal inocula (gilts and multiparous sows) were assessed through staticin vitrocolonic-digestion simulations with rapeseed. After derivatization and extraction of the fecal suspensions, TU was analyzed using liquid chromatography-tandem mass spectrometry (LC-MS2). On average, lower TU concentrations were observed in fecal colonic simulations in gilts (8.35 ng g−1rapeseed ± 3.42 [mean ± standard deviation]) than in multiparous sows (52.63 ng g−1± 16.17), which correlates with maturation of the gut microbial population with age. Further exploration of the mechanism showed cell-dependent activity of the microbial conversion and sustained TU-forming activity after subjection of the fecal inoculum to moderate heat over a time span of up to 30 min. Finally, nine TU-producing bacterial species were successfully isolated and identified by a combination of biochemical and molecular techniques asEscherichia coli(n= 5),Lactobacillus reuteri(n= 2),Enterococcus faecium(n= 1), andSalmonella entericasubsp.arizonae(n= 1). This report demonstrates that endogenous formation of TU is Brassicaceae induced and occurs under colonic conditions most likely through myrosinase-like enzyme activity expressed by different common intestinal bacterial species.

scholarly journals Mechanistic Insight into Size-Dependent Activity and Durability in Pt/CNT Catalyzed Hydrolytic Dehydrogenation of Ammonia Borane

2014 ◽  
Vol 136 (48) ◽  
pp. 16736-16739 ◽  
Author(s):  
Wenyao Chen ◽  
Jian Ji ◽  
Xiang Feng ◽  
Xuezhi Duan ◽  
Gang Qian ◽  
...  
Keyword(s):  
Ammonia Borane ◽  
Size Dependent ◽  
Mechanistic Insight ◽  
Dependent Activity ◽  
Insight Into

Calibration problems in hydrogen luminosities

1966 ◽  
Vol 24 ◽  
pp. 322-330
Author(s):  
A. Beer
Keyword(s):  
Galactic Structure ◽  
B Stars ◽  
Insight Into

The investigations which I should like to summarize in this paper concern recent photo-electric luminosity determinations of O and B stars. Their final aim has been the derivation of new stellar distances, and some insight into certain patterns of galactic structure.

Stochasting modeling of planetary ring systems

1984 ◽  
Vol 75 ◽  
pp. 461-469 ◽  
Author(s):  
Robert W. Hart
Keyword(s):  
Maximum Entropy ◽  
Ring System ◽  
The Sun ◽  
Ultimate State ◽  
Interparticle Interactions ◽  
Ring Systems ◽  
First Order ◽  
Planetary Ring ◽  
Insight Into

ABSTRACTThis paper models maximum entropy configurations of idealized gravitational ring systems. Such configurations are of interest because systems generally evolve toward an ultimate state of maximum randomness. For simplicity, attention is confined to ultimate states for which interparticle interactions are no longer of first order importance. The planets, in their orbits about the sun, are one example of such a ring system. The extent to which the present approximation yields insight into ring systems such as Saturn's is explored briefly.

Skeletal elements of crinoid echinoderms: High-resolution structural and microanalytical results

1983 ◽  
Vol 41 ◽  
pp. 194-195
Author(s):  
D. F. Blake ◽  
L. F. Allard ◽  
D. R. Peacor
Keyword(s):  
High Resolution ◽  
Marine Invertebrates ◽  
Sea Urchins ◽  
Structural Features ◽  
Sea Stars ◽  
High Resolution Tem ◽  
Relationship Of ◽  
The Relationship ◽  
Insight Into

Echinodermata is a phylum of marine invertebrates which has been extant since Cambrian time (c.a. 500 m.y. before the present). Modern examples of echinoderms include sea urchins, sea stars, and sea lilies (crinoids). The endoskeletons of echinoderms are composed of plates or ossicles (Fig. 1) which are with few exceptions, porous, single crystals of high-magnesian calcite. Despite their single crystal nature, fracture surfaces do not exhibit the near-perfect {10.4} cleavage characteristic of inorganic calcite. This paradoxical mix of biogenic and inorganic features has prompted much recent work on echinoderm skeletal crystallography. Furthermore, fossil echinoderm hard parts comprise a volumetrically significant portion of some marine limestones sequences. The ultrastructural and microchemical characterization of modern skeletal material should lend insight into: 1). The nature of the biogenic processes involved, for example, the relationship of Mg heterogeneity to morphological and structural features in modern echinoderm material, and 2). The nature of the diagenetic changes undergone by their ancient, fossilized counterparts. In this study, high resolution TEM (HRTEM), high voltage TEM (HVTEM), and STEM microanalysis are used to characterize tha ultrastructural and microchemical composition of skeletal elements of the modern crinoid Neocrinus blakei.

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