Ordering Transitions in Liquid Crystals Triggered by Bioactive Cyclic Amphiphiles: Potential Application in Label-Free Detection of Amyloidogenic Peptides

2019 ◽  
Vol 123 (11) ◽  
pp. 6526-6536 ◽  
Author(s):  
Indu Verma ◽  
Neelima Rajeev ◽  
Golam Mohiuddin ◽  
Santanu Kumar Pal
Keyword(s):  
Liquid Crystals ◽  
Potential Application ◽  
Label Free ◽  
Transitions In Liquid Crystals ◽  
Label Free Detection ◽  
Ordering Transitions ◽  
Amyloidogenic Peptides

A novel method for label-free detection of ricin using liquid crystals supported on chemically functionalized surfaces

2011 ◽  
Vol 155 (1) ◽  
pp. 351-356 ◽  
Author(s):  
Ya-Bin Zhao ◽  
Jian-Hua Yu ◽  
Hong-Fei Zhao ◽  
Chao-Yang Tong ◽  
Pu-Hong Wang
Keyword(s):  
Liquid Crystals ◽  
Label Free ◽  
Functionalized Surfaces ◽  
Label Free Detection ◽  
Novel Method

scholarly journals Label-Free Detection and Spectrometrically Quantitative Analysis of the Cancer Biomarker CA125 Based on Lyotropic Chromonic Liquid Crystal

Biosensors ◽  
2021 ◽  
Vol 11 (8) ◽  
pp. 271
Author(s):  
Hassanein Shaban ◽  
Mon-Juan Lee ◽  
Wei Lee
Keyword(s):  
Liquid Crystals ◽  
Limit Of Detection ◽  
Optical Signal ◽  
Cancer Biomarker ◽  
Disodium Cromoglycate ◽  
Label Free ◽  
Light Spectrum ◽  
Linear Calibration ◽  
Label Free Detection ◽  
Anchoring Strength

Compared with thermotropic liquid crystals (LCs), the biosensing potential of lyotropic chromonic liquid crystals (LCLCs), which are more biocompatible because of their hydrophilic nature, has scarcely been investigated. In this study, the nematic phase, a mesophase shared by both thermotropic LCs and LCLCs, of disodium cromoglycate (DSCG) was employed as the sensing mesogen in the LCLC-based biosensor. The biosensing platform was constructed so that the LCLC was homogeneously aligned by the planar anchoring strength of polyimide, but was disrupted in the presence of proteins such as bovine serum albumin (BSA) or the cancer biomarker CA125 captured by the anti-CA125 antibody, with the level of disturbance (and the optical signal thus produced) predominated by the amount of the analyte. The concentration- and wavelength-dependent optical response was analyzed by transmission spectrometry in the visible light spectrum with parallel or crossed polarizers. The concentration of CA125 can be quantified with spectrometrically derived parameters in a linear calibration curve. The limit of detection for both BSA and CA125 of the LCLC-based biosensor was superior or comparable to that of thermotropic LC-based biosensing techniques. Our results provide, to the best of our knowledge, the first evidence that LCLCs can be applied in spectrometrically quantitative biosensing.

scholarly journals The Role of Surface Chemistry in the Efficacy of Protein and DNA Microarrays for Label-Free Detection: An Overview

Polymers ◽  
2021 ◽  
Vol 13 (7) ◽  
pp. 1026
Author(s):  
Elisa Chiodi ◽  
Allison M. Marn ◽  
Matthew T. Geib ◽  
M. Selim Ünlü
Keyword(s):  
Surface Chemistry ◽  
Surface Functionalization ◽  
Dna Microarrays ◽  
Label Free ◽  
Polymeric Coatings ◽  
Label Free Detection ◽  
Particle Imaging ◽  
Single Particle Imaging ◽  
The Impact

The importance of microarrays in diagnostics and medicine has drastically increased in the last few years. Nevertheless, the efficiency of a microarray-based assay intrinsically depends on the density and functionality of the biorecognition elements immobilized onto each sensor spot. Recently, researchers have put effort into developing new functionalization strategies and technologies which provide efficient immobilization and stability of any sort of molecule. Here, we present an overview of the most widely used methods of surface functionalization of microarray substrates, as well as the most recent advances in the field, and compare their performance in terms of optimal immobilization of the bioreceptor molecules. We focus on label-free microarrays and, in particular, we aim to describe the impact of surface chemistry on two types of microarray-based sensors: microarrays for single particle imaging and for label-free measurements of binding kinetics. Both protein and DNA microarrays are taken into consideration, and the effect of different polymeric coatings on the molecules’ functionalities is critically analyzed.

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