Fluorescence Quenching-Based Evaluation of Glucose Oxidase Composite with Conducting Polymer, Polypyrrole

2018 ◽  
Vol 122 (17) ◽  
pp. 9491-9498 ◽  
Author(s):  
Urte Bubniene ◽  
Raminta Mazetyte ◽  
Almira Ramanaviciene ◽  
Vidmantas Gulbinas ◽  
Arunas Ramanavicius ◽  
...  
Keyword(s):  
Fluorescence Quenching ◽  
Glucose Oxidase ◽  
Conducting Polymer

Immunosensor based on fluorescence quenching matrix of the conducting polymer polypyrrole

2010 ◽  
Vol 398 (7-8) ◽  
pp. 3105-3113 ◽  
Author(s):  
A. Ramanavicius ◽  
N. Ryskevic ◽  
Y. Oztekin ◽  
A. Kausaite-Minkstimiene ◽  
S. Jursenas ◽  
...  
Keyword(s):  
Fluorescence Quenching ◽  
Conducting Polymer

scholarly journals Dispersed Conducting Polymer Nanocomposites with Glucose Oxidase and Gold Nanoparticles for the Design of Enzymatic Glucose Biosensors

Polymers ◽  
2021 ◽  
Vol 13 (13) ◽  
pp. 2173
Author(s):  
Natalija German ◽  
Almira Ramanaviciene ◽  
Arunas Ramanavicius
Keyword(s):  
Glucose Oxidase ◽  
Conducting Polymer ◽  
Limit Of Detection ◽  
High Sensitivity ◽  
Serum Samples ◽  
Glucose Determination ◽  
Constant Potential ◽  
Good Repeatability ◽  
Graphite Rod

Biosensors for the determination of glucose concentration have a great significance in clinical diagnosis, and in the food and pharmaceutics industries. In this research, short-chain polyaniline (PANI) and polypyrrole (Ppy)-based nanocomposites with glucose oxidase (GOx) and 6 nm diameter AuNPs (AuNPs(6 nm)) were deposited on the graphite rod (GR) electrode followed by the immobilization of GOx. Optimal conditions for the modification of GR electrodes by conducting polymer-based nanocomposites and GOx were elaborated. The electrodes were investigated by cyclic voltammetry and constant potential amperometry in the presence of the redox mediator phenazine methosulfate (PMS). The improved enzymatic biosensors based on GR/PANI-AuNPs(6 nm)-GOx/GOx and GR/Ppy-AuNPs(6 nm)-GOx/GOx electrodes were characterized by high sensitivity (65.4 and 55.4 μA mM−1 cm−2), low limit of detection (0.070 and 0.071 mmol L−1), wide linear range (up to 16.5 mmol L−1), good repeatability (RSD 4.67 and 5.89%), and appropriate stability (half-life period (τ1/2) was 22 and 17 days, respectively). The excellent anti-interference ability to ascorbic and uric acids and successful practical application for glucose determination in serum samples was presented for GR/PANI-AuNPs(6 nm)-GOx/GOx electrode.

Sign in / Sign up

Export Citation Format

Share Document