Lifting the Spectral Crosstalk in Multifluorophore Assemblies

2017 ◽  
Vol 121 (47) ◽  
pp. 26226-26232 ◽  
Author(s):  
Pavel Moroz ◽  
William P. Klein ◽  
Kiernan Akers ◽  
Abigail Vore ◽  
Natalia Kholmicheva ◽  
...  
Keyword(s):  
Spectral Crosstalk

Application of Spectral Crosstalk Correction for Improving Multiplexed MicroRNA Detection Using a Single Excitation Wavelength

2017 ◽  
Vol 89 (6) ◽  
pp. 3430-3436 ◽  
Author(s):  
Yuanjian Liu ◽  
Min Wei ◽  
Ying Li ◽  
Anran Liu ◽  
Wei Wei ◽  
...  
Keyword(s):  
Excitation Wavelength ◽  
Microrna Detection ◽  
Spectral Crosstalk ◽  
Crosstalk Correction

Spectral crosstalk by radiative recombination in sequential-mode, dual mid-wavelength infrared band HgCdTe detectors

2004 ◽  
Vol 33 (6) ◽  
pp. 517-525 ◽  
Author(s):  
R. A. Coussa ◽  
A. M. Gallagher ◽  
K. Kosai ◽  
L. T. Pham ◽  
G. K. Pierce ◽  
...  
Keyword(s):  
Radiative Recombination ◽  
Infrared Band ◽  
Sequential Mode ◽  
Spectral Crosstalk

Studies of the spectral crosstalk in two-color IR projector systems

2003 ◽  
Author(s):  
Richard B. Sisko ◽  
David S. Flynn ◽  
Breck A. Sieglinger ◽  
James D. Norman
Keyword(s):  
Spectral Crosstalk

Partial Acceptor Photobleaching-Based Quantitative FRET Method Completely Overcoming Emission Spectral Crosstalks

2012 ◽  
Vol 18 (5) ◽  
pp. 1021-1029 ◽  
Author(s):  
Huali Li ◽  
Huaina Yu ◽  
Tongsheng Chen
Keyword(s):  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Correction Method ◽  
Living Cells ◽  
Acceptor Pair ◽  
Donor And Acceptor ◽  
Acceptor Photobleaching ◽  
Before And After ◽  
The Absolute ◽  
Spectral Crosstalk

AbstractBased on the quantitative fluorescence resonance energy transfer (FRET) method named PbFRET we reported recently, we herein developed a partial acceptor photobleaching-based quantitative FRET algorithm named B-PbFRET method. B-PbFRET overcomes not only the acceptor excitation crosstalk and donor emission spectral crosstalk but also the acceptor emission spectral crosstalk that harasses previous methods including fluorescence lifetime (FLIM), fluorescence recovery of donor after acceptor photobleaching, and acceptor sensitized emission (SE)-based methods. B-PbFRET method is implemented by simultaneously measuring the fluorescence intensity of both donor and acceptor channels at donor excitation before and after partial acceptor photobleaching, and it can directly measure the FRET efficiency (E) without any verified references. Based on the theoretical analysis of B-PbFRET, we also developed a more straightforward correction method named C-PbFRET to obtain the absolute E from the value measured by PbFRET for a given donor-acceptor pair. We validated both B-PbFRET and C-PbFRET methods by measuring the E of two linked constructs, 18AA and SCAT3 proteins, in single living cells, and our data demonstrated that both B-PbFRET and C-PbFRET methods can directly measure the absolute E of the linked constructs inside living cells under different degrees of acceptor emission spectral crosstalk.

Modeling and mitigation of spectral crosstalk in OFDM WDM-VLC system

2021 ◽  
Vol 478 ◽  
pp. 126361
Author(s):  
Luis Carlos Mathias ◽  
Leonimer Flávio de Melo ◽  
Taufik Abrao
Keyword(s):  
Spectral Crosstalk
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