scholarly journals Effect of Silver Nanoparticles on the Photophysics of Riboflavin: Consequences on the ROS Generation

2016 ◽  
Vol 120 (38) ◽  
pp. 21967-21975 ◽  
Author(s):  
María Belén Rivas Aiello ◽  
Juan José Romero ◽  
Sonia G. Bertolotti ◽  
Mónica C. Gonzalez ◽  
Daniel O. Mártire
Keyword(s):  
Silver Nanoparticles ◽  
Ros Generation

scholarly journals Correction to “Effect of Silver Nanoparticles on the Photophysics of Riboflavin: Consequences on the ROS Generation”

2016 ◽  
Vol 120 (44) ◽  
pp. 25638-25638
Author(s):  
María Belén Rivas Aiello ◽  
Juan José Romero ◽  
Sonia G. Bertolotti ◽  
Mónica C. Gonzalez ◽  
Daniel O. Mártire
Keyword(s):  
Silver Nanoparticles ◽  
Ros Generation

scholarly journals Silver Nanoparticles Synthesized with Rumex hymenosepalus: A Strategy to Combat Early Mortality Syndrome (EMS) in a Cultivated White Shrimp

2019 ◽  
Vol 2019 ◽  
pp. 1-15 ◽  
Author(s):  
Francisco Javier Alvarez-Cirerol ◽  
Marco Antonio López-Torres ◽  
Ericka Rodríguez-León ◽  
César Rodríguez-Beas ◽  
Aaron Martínez-Higuera ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Silver Nanoparticles ◽  
Antibacterial Properties ◽  
Early Mortality ◽  
White Shrimp ◽  
Ros Generation ◽  
Early Mortality Syndrome ◽  
Selected Area Electron Diffraction ◽  
Worldwide Production ◽  
Vis Spectroscopy

Early Mortality Syndrome (EMS) or Acute Hepatopancreatic Necrosis Syndrome (AHPNS) is a disease produced by gram-negative bacteria Vibrio parahaemolyticus (V. parahaemolyticus), which has caused declines in worldwide production of a white shrimp Litopenaeus vannamei (L. vannamei). In this work, we propose the implementation of silver nanoparticles (AgNPs) synthesized with Rumex hymenosepalus (Rh) extract as an alternative on V. parahaemolyticus control. AgNPs were characterized by UV-Vis spectroscopy, scanning electron microscopy (SEM), transmission electron microscopy (TEM), and selected area electron diffraction (SAED). AgNP mean sizes by DLS were 80.82±1.16 nm and sizes between 2 and 10 nm by TEM, with a zeta potential of −47.72±1.05 mV. This study evaluated AgNPs and Rh antimicrobial capacity on V. parahaemolyticus at different concentrations; the minimum inhibitory concentration (MIC) found was 25 μg/mL for AgNPs and 220 μg/mL for Rh. Additionally, were carried out time-kill curves and reactive oxygen species (ROS) generation for 1 and 4 MIC. Both concentrations (MIC) were tested for toxicity on Artemia nauplii from Artemia franciscana (A. franciscana), because nauplii were used as biocarriers for AgNPs and Rh extract on L. vannamei. Once the shrimp were treated, they were challenged with Vibrio infection and it was found that those who were treated with both agents showed greater survival than the control. V. parahaemolyticus and postlarval samples were taken from the bioassay and fixed and prepared for TEM and SEM in order to search NPs in internal structure of bacteria and the hepatopancreatic area of shrimps; AgNPs were detected in both cases. AgNPs and Rh extract show antibacterial properties on the infected shrimp with V. parahaemolyticus. The action mechanisms are interaction with the bacterial membrane and ROS generation; these effects are produced by both agents.

Effects of Chloride Ions on Dissolution, ROS Generation, and Toxicity of Silver Nanoparticles under UV Irradiation

2017 ◽  
Vol 52 (8) ◽  
pp. 4842-4849 ◽  
Author(s):  
Yang Li ◽  
Jian Zhao ◽  
Enxiang Shang ◽  
Xinghui Xia ◽  
Junfeng Niu ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Uv Irradiation ◽  
Chloride Ions ◽  
Ros Generation

scholarly journals Cytotoxic Potential and Molecular Pathway Analysis of Silver Nanoparticles in Human Colon Cancer Cells HCT116

2018 ◽  
Vol 19 (8) ◽  
pp. 2269 ◽  
Author(s):  
Sangiliyandi Gurunathan ◽  
Muhammad Qasim ◽  
Chanhyeok Park ◽  
Hyunjin Yoo ◽  
Jin-Hoi Kim ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Cell Cycle ◽  
Colon Cancer ◽  
Dna Damage ◽  
Silver Nanoparticles ◽  
Cancer Cells ◽  
Ros Generation ◽  
Dead Cell ◽  
Colon Cancer Cells ◽  
Colorectal Cancer Cells

Silver nanoparticles (AgNPs) have gained attention for use in cancer therapy. In this study, AgNPs were biosynthesized using naringenin. We investigated the anti-colon cancer activities of biogenic AgNPs through transcriptome analysis using RNA sequencing, and the mechanisms of AgNPs in regulating colon cancer cell growth. The synthesized AgNPs were characterized using UV–visible spectroscopy (UV–vis), X-ray diffraction (XRD), Fourier-transform infrared spectroscopy (FTIR), dynamic light scattering (DLS), and transmission electron microscopy (TEM). The AgNPs were spherical with sizes of 2–10 nm. Cytotoxicity assays indicated that the AgNPs in HCT116 colorectal cancer cells were very effective at low concentrations. The viability and proliferation of colon cancer cells treated with 5 µg/mL biogenic AgNPs were reduced by 50%. Increased lactate dehydrogenase leakage (LDH), reactive oxygen species (ROS) generation, malondialdehyde (MDA), and decreased dead-cell protease activity and ATP generation were observed. This impaired mitochondrial function and DNA damage led to cell death. The AgNPs upregulated and downregulated the most highly ranked biological processes of oxidation–reduction and cell-cycle regulation, respectively. Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis showed that AgNPs upregulated GADD45G in the p53 pathway. Thus, the AgNP tumor suppressive effects were mediated by cell apoptosis following DNA damage, as well as by mitochondrial dysfunction and cell-cycle arrest following aberrant regulation of p53 effector proteins. It is of interest to mention that, to the best of our knowledge, this study is the first report demonstrating cellular responses and molecular pathways analysis of AgNPs in HCT116 colorectal cancer cells.

scholarly journals Cytotoxicity of Biologically Synthesized Silver Nanoparticles in MDA-MB-231 Human Breast Cancer Cells

2013 ◽  
Vol 2013 ◽  
pp. 1-10 ◽  
Author(s):  
Sangiliyandi Gurunathan ◽  
Jae Woong Han ◽  
Vasuki Eppakayala ◽  
Muniyandi Jeyaraj ◽  
Jin-Hoi Kim
Keyword(s):  
Breast Cancer ◽  
Silver Nanoparticles ◽  
Cancer Cells ◽  
Human Breast Cancer ◽  
Breast Cancer Cells ◽  
Ros Generation ◽  
Dependent Manner ◽  
Limited Information ◽  
Human Breast ◽  
Dose Dependent

Silver nanoparticles (AgNPs) have been used as an antimicrobial and disinfectant agents. However, there is limited information about antitumor potential. Therefore, this study focused on determining cytotoxic effects of AgNPs on MDA-MB-231 breast cancer cells and its mechanism of cell death. Herein, we developed a green method for synthesis of AgNPs using culture supernatant ofBacillus funiculus, and synthesized AgNPs were characterized by various analytical techniques such as UV-visible spectrophotometer, particle size analyzer, and transmission electron microscopy (TEM). The toxicity was evaluated using cell viability, metabolic activity, and oxidative stress. MDA-MB-231 breast cancer cells were treated with various concentrations of AgNPs (5 to 25 μg/mL) for 24 h. We found that AgNPs inhibited the growth in a dose-dependent manner using MTT assay. AgNPs showed dose-dependent cytotoxicity against MDA-MB-231 cells through activation of the lactate dehydrogenase (LDH), caspase-3, reactive oxygen species (ROS) generation, eventually leading to induction of apoptosis which was further confirmed through resulting nuclear fragmentation. The present results showed that AgNPs might be a potential alternative agent for human breast cancer therapy.

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