Biofunctionality of Calmodulin Immobilized on Gold Surface Studied by Surface-Enhanced Infrared Absorption Spectroscopy: Ca2+-Induced Conformational Change and Binding to a Target Peptide

2016 ◽  
Vol 120 (29) ◽  
pp. 16035-16041 ◽  
Author(s):  
Hidenori Noguchi ◽  
Tatsuhiko Adachi ◽  
Akiko Nakatomi ◽  
Michio Yazawa ◽  
Kohei Uosaki
Keyword(s):  
Conformational Change ◽  
Absorption Spectroscopy ◽  
Infrared Absorption ◽  
Gold Surface ◽  
Infrared Absorption Spectroscopy ◽  
Target Peptide ◽  
Surface Enhanced

A Two-Layer Gold Surface with Improved Surface Enhancement for Spectro-Electrochemistry Using Surface-Enhanced Infrared Absorption Spectroscopy

2009 ◽  
Vol 63 (9) ◽  
pp. 1068-1074 ◽  
Author(s):  
C. Nowak ◽  
C. Luening ◽  
W. Knoll ◽  
R. L. C. Naumann
Keyword(s):  
Cytochrome C ◽  
Absorption Spectroscopy ◽  
Infrared Absorption ◽  
Gold Surface ◽  
Growth Conditions ◽  
Infrared Absorption Spectroscopy ◽  
Optimum Conditions ◽  
Self Assembled Monolayer ◽  
Surface Enhanced ◽  
Reversible Reduction

A two-layer gold surface is developed for use with surface-enhanced infrared absorption spectroscopy (SEIRAS) consisting of a conducting underlayer onto which Au nanoparticles (AuNPs) are grown by self-catalyzed electroless deposition. AuNPs are grown on protruding substructures of the 25 nm thin underlayer. The enhancement factor of the two-layer gold surface is controlled by the growth conditions. Cytochrome c adsorbed to a self-assembled monolayer of mercaptoethanol is used as a reference system. Under optimum conditions the absorbance of the amide I band is increased by a factor of 5 versus the classical SEIRAS surface. Reversible reduction/oxidation of cytochrome c on the two-layer gold surface is shown to take place by cyclic voltammetry.

Surfactant-assisted electroless deposition of silver nanoparticles on Ge crystal for ultra-sensitive detection by surface-enhanced infrared absorption spectroscopy

RSC Advances ◽  
2015 ◽  
Vol 5 (26) ◽  
pp. 20390-20395 ◽  
Author(s):  
Gadupudi Purna Chandra Rao ◽  
Vairaperumal Tharmaraj ◽  
Jyisy Yang
Keyword(s):  
Silver Nanoparticles ◽  
Absorption Spectroscopy ◽  
Infrared Absorption ◽  
Electroless Deposition ◽  
Sensitive Detection ◽  
Infrared Absorption Spectroscopy ◽  
Highly Sensitive ◽  
Surface Enhanced ◽  
Surfactant Assisted

Fabrication of highly sensitive substrates for SEIRA measurements via assistance of surfactant to electrolessly deposit silver nanoparticles on Ge crystal.

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