scholarly journals Chaotropic Agents Boosting the Performance of Photoionic Cells

2015 ◽  
Vol 119 (9) ◽  
pp. 4728-4735 ◽  
Author(s):  
Romain Bourdon ◽  
Pekka Peljo ◽  
Manuel A. Méndez ◽  
Astrid J. Olaya ◽  
Jelissa De Jonghe-Risse ◽  
...  
Keyword(s):  
Chaotropic Agents

scholarly journals Evaluation of Chemical Protocols for Inactivating SARS-CoV-2 Infectious Samples

Viruses ◽  
2020 ◽  
Vol 12 (6) ◽  
pp. 624 ◽  
Author(s):  
Boris Pastorino ◽  
Franck Touret ◽  
Magali Gilles ◽  
Lea Luciani ◽  
Xavier de Lamballerie ◽  
...  
Keyword(s):  
Culture Supernatant ◽  
Rna Extraction ◽  
Cell Culture Supernatant ◽  
Molecular Diagnostic ◽  
Clinical Samples ◽  
European Standard ◽  
Lysis Buffer ◽  
Level 2 ◽  
Biosafety Level ◽  
Chaotropic Agents

Clinical samples collected in coronavirus disease 19 (COVID-19), patients are commonly manipulated in biosafety level 2 laboratories for molecular diagnostic purposes. Here, we tested French norm NF-EN-14476+A2 derived from European standard EN-14885 to assess the risk of manipulating infectious viruses prior to RNA extraction. SARS-CoV-2 cell-culture supernatant and nasopharyngeal samples (virus-spiked samples and clinical samples collected in COVID-19 patients) were used to measure the reduction of infectivity after 10 min contact with lysis buffer containing various detergents and chaotropic agents. A total of thirteen protocols were evaluated. Two commercially available formulations showed the ability to reduce infectivity by at least 6 log 10, whereas others proved less effective.

Effective interactions between chaotropic agents and proteins

2005 ◽  
Vol 61 (3) ◽  
pp. 492-499 ◽  
Author(s):  
Giovanni Salvi ◽  
Paolo De Los Rios ◽  
Michele Vendruscolo
Keyword(s):  
Effective Interactions ◽  
Chaotropic Agents

Metal island coated polymer sensor for direct determination of the volume effect of chaotropic agents

1995 ◽  
Vol 121 (1-4) ◽  
pp. 259-268 ◽  
Author(s):  
Th. Schalkhammer ◽  
Ch. Lobmaier ◽  
F. Pittner ◽  
A. Leitner ◽  
H. Brunner ◽  
...  
Keyword(s):  
Direct Determination ◽  
Volume Effect ◽  
Metal Island ◽  
Chaotropic Agents

scholarly journals Glucose dehydrogenase from the thermoacidophilic archaebacterium Sulfolobus solfataricus

1986 ◽  
Vol 239 (3) ◽  
pp. 517-522 ◽  
Author(s):  
P Giardina ◽  
M G de Biasi ◽  
M de Rosa ◽  
A Gambacorta ◽  
V Buonocore
Keyword(s):  
Oxidation Rate ◽  
Glucose Dehydrogenase ◽  
Sulfolobus Solfataricus ◽  
Maximal Activity ◽  
Equatorial Orientation ◽  
Guanidinium Chloride ◽  
Cell Extracts ◽  
Hydroxy Groups ◽  
Acid Substrate ◽  
Chaotropic Agents

Glucose dehydrogenase has been purified to homogeneity from cell extracts of the extreme thermoacidophilic archaebacterium Sulfolobus solfataricus. The enzyme utilizes both NAD+ and NADP+ as coenzyme and catalyses the oxidation of several monosaccharides to the corresponding glyconic acid. Substrate specificity and oxidation rate depend on the coenzyme present; when NAD+ is used, the enzyme binds and oxidizes specifically sugars presenting equatorial orientation of hydroxy groups at C-2, C-3 and C-4. The Mr of the native enzyme is 124,000 and decreases to about 60,000 in the presence of 6 M-guanidinium chloride and to about 30,000 in the presence of 5% (w/v) SDS. The enzyme shows maximal activity at pH 9, 77 degrees C and 20 mM-Mg2+, -Mn2+ or -Ca2+ and is fairly stable in the presence of chaotropic agents and water-miscible organic solvents such as methanol or acetone.

scholarly journals Correction: Structural stability of DNA origami nanostructures in the presence of chaotropic agents

Nanoscale ◽  
2017 ◽  
Vol 9 (24) ◽  
pp. 8508-8508
Author(s):  
Saminathan Ramakrishnan ◽  
Georg Krainer ◽  
Guido Grundmeier ◽  
Michael Schlierf ◽  
Adrian Keller
Keyword(s):  
Structural Stability ◽  
Dna Origami ◽  
Chaotropic Agents

Fluorescence Microscopic Study of Microorganisms Treated with Chaotropic Agents

Microbiology ◽  
2005 ◽  
Vol 74 (4) ◽  
pp. 434-439
Author(s):  
V. I. Duda ◽  
V. N. Danilevich ◽  
V. N. Akimov ◽  
N. E. Suzina ◽  
V. V. Dmitriev ◽  
...  
Keyword(s):  
Microscopic Study ◽  
Chaotropic Agents
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