Metallic Nanofilm Enhanced Fluorescence Cell Imaging: A Study of Distance-Dependent Intensity and Lifetime by Optical Sectioning Microscopy

2020 ◽  
Vol 124 (14) ◽  
pp. 2760-2768
Author(s):  
Yan-Yun Zhai ◽  
Qian Liu ◽  
Wei-Peng Cai ◽  
Shuo-Hui Cao ◽  
Li-Xiang Zhang ◽  
...  
Keyword(s):  
Cell Imaging ◽  
Optical Sectioning ◽  
Enhanced Fluorescence ◽  
Metallic Nanofilm

scholarly journals Challenges in 3D Live Cell Imaging

Photonics ◽  
2021 ◽  
Vol 8 (7) ◽  
pp. 275
Author(s):  
Herbert Schneckenburger ◽  
Verena Richter
Keyword(s):  
Live Cell Imaging ◽  
Laser Scanning ◽  
Cell Imaging ◽  
Live Cell ◽  
Laser Scanning Microscopy ◽  
Radiative Energy ◽  
Wide Field ◽  
Optical Sectioning ◽  
Continuous Increase ◽  
Radiative Energy Transfer

A short overview on 3D live cell imaging is given. Relevant samples are described and various problems and challenges—including 3D imaging by optical sectioning, light scattering and phototoxicity—are addressed. Furthermore, enhanced methods of wide-field or laser scanning microscopy together with some relevant examples and applications are summarized. In the future one may profit from a continuous increase in microscopic resolution, but also from molecular sensing techniques in the nanometer range using e.g., non-radiative energy transfer (FRET).

scholarly journals Monitoring individual cell-signaling activity using combined metal-clad waveguide and surface-enhanced fluorescence imaging

The Analyst ◽  
2018 ◽  
Vol 143 (22) ◽  
pp. 5559-5567 ◽  
Author(s):  
Thomas Söllradl ◽  
Kevin Chabot ◽  
Ulrike Fröhlich ◽  
Michael Canva ◽  
Paul G. Charette ◽  
...  
Keyword(s):  
Cell Signaling ◽  
Fluorescence Imaging ◽  
Live Cell Imaging ◽  
Cell Imaging ◽  
Individual Cell ◽  
Live Cell ◽  
Enhanced Fluorescence ◽  
Surface Enhanced Fluorescence ◽  
Surface Enhanced ◽  
Signaling Activity

Validation of a combined metal-clad waveguide and surface enhanced fluorescence imaging platform for live cell imaging.

A novel fluorescent probe for Hg2+ detection in a wide pH range and its application in living cell imaging

2018 ◽  
Vol 10 (46) ◽  
pp. 5554-5558 ◽  
Author(s):  
Jing Xu ◽  
Honglin Li ◽  
Yanchi Chen ◽  
Bing Yang ◽  
Qingcai Jiao ◽  
...  
Keyword(s):  
Amino Acids ◽  
Metal Ions ◽  
Fluorescent Probe ◽  
Living Cell ◽  
Cell Imaging ◽  
Enhanced Fluorescence ◽  
Wide Ph Range ◽  
Ph Range ◽  
Living Cell Imaging

A novel fluorescent probe (XL-1) exhibited over 50-fold enhanced fluorescence in the presence of Hg2+ and no interference could be produced by other metal ions, anions and amino acids.

Enhanced-fluorescence of europium–copper nanoclusters for cell imaging

2016 ◽  
Vol 51 (15) ◽  
pp. 7229-7235 ◽  
Author(s):  
Hong Miao ◽  
Yuanjiao Feng ◽  
Dan Zhong ◽  
Xiaoming Yang
Keyword(s):  
Cell Imaging ◽  
Enhanced Fluorescence ◽  
Copper Nanoclusters

A novel peptide-based fluorescent chemosensor for measuring zinc ions using different excitation wavelengths and application in live cell imaging

2015 ◽  
Vol 3 (17) ◽  
pp. 3617-3624 ◽  
Author(s):  
Peng Wang ◽  
Jiang Wu ◽  
Panpan Zhou ◽  
Weisheng Liu ◽  
Yu Tang
Keyword(s):  
Aqueous Solution ◽  
Live Cell Imaging ◽  
Cell Imaging ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Living Cells ◽  
Fluorescent Chemosensor ◽  
Zinc Ions ◽  
Enhanced Fluorescence ◽  
Chelation Enhanced Fluorescence

A novel peptide-based fluorescent chemosensor containing both tryptophan and a dansyl fluorophore has been designed to detect Zn2+ in 100% aqueous solution and living cells via two pathways including fluorescence resonance energy transfer and chelation enhanced fluorescence.

Substituent enhanced fluorescence properties of star α-cyanostilbenes and their application in bioimaging

2019 ◽  
Vol 7 (39) ◽  
pp. 6010-6023 ◽  
Author(s):  
A. Gopinath ◽  
N. Manivannan ◽  
Sudip Mandal ◽  
N. Mathivanan ◽  
A. Sultan Nasar
Keyword(s):  
Cell Imaging ◽  
Fluorescence Properties ◽  
Imaging Studies ◽  
Nuclear Movement ◽  
Enhanced Fluorescence

In this paper, we report the fluorescence properties of new star α-cyanostilbene molecules. Fungus cell imaging studies using one of the molecules allowed observing nuclear movement in the live mycelium.

scholarly journals Enhanced fluorescence cell imaging with metal-coated slides

2007 ◽  
Author(s):  
Eric Le Moal ◽  
Emmanuel Fort ◽  
Sandrine Lévêque-Fort ◽  
Anne Janin ◽  
Hideyuki Murata ◽  
...  
Keyword(s):  
Cell Imaging ◽  
Enhanced Fluorescence

High Speed Two Photon Excitation Microscopy in Live Cell Imaging using Image Correlation Spectroscopy (ICS)

2001 ◽  
Vol 7 (S2) ◽  
pp. 22-23
Author(s):  
P. W. Wiseman ◽  
J. C. Bouwer ◽  
S. Peltier ◽  
M. H. Ellisman
Keyword(s):  
Live Cell Imaging ◽  
High Speed ◽  
Cell Imaging ◽  
Live Cell ◽  
Image Correlation ◽  
Scanning System ◽  
Optical Sectioning ◽  
Two Photon ◽  
Photon Excitation ◽  
Two Photon Excitation

For live-cell imaging, two-photon excitation microscopy (TPEM) is proving to be a significant technological advancement. The unique features offered by TPEM are the ability to image thick sections, excellent optical sectioning capabilities, low damage to living cells, and less out of focus fluorescence and out of focus photobleaching. of these features, the most useful for the biological microscopist, is optical sectioning. Optical sectioning is an intrinsic property of the two-photon process, whereby, two infrared (IR) photons are absorbed quickly to excite a single UV/blue transition. The probability for exciting a two photon transition is proportional to the instantaneous excitation intensity squared. Therefore, for a focused laser beam, only light at the focal point of the excitation beam excites a fluorescent transition. Thus, the need for confocal apertures and time consuming deconvolution algorithms are, for the most part, eliminated.We have continued to develop and enhance our ability to perform high-speed, two-photon excitation fluorescence microscopy. in 1998, we successfully deployed a prototype, video-rate twophoton laser scanning system (30 frames/sec or faster at reduced scan width) developed with support from Nikon Corporation. That system was built upon a Nikon RCM 8000 confocal microscope.

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