In Silico Discovery and Validation of Neuropeptide-Y-Binding Peptides for Sensors

2019 ◽  
Vol 124 (1) ◽  
pp. 61-68 ◽  
Author(s):  
Xingqing Xiao ◽  
Zhifeng Kuang ◽  
B. J. Burke ◽  
Yaroslav Chushak ◽  
Barry L. Farmer ◽  
...  
Keyword(s):  
Neuropeptide Y ◽  
In Silico ◽  
Binding Peptides

scholarly journals Targeting PirAvp and PirBvp Toxins of Vibrio parahaemolyticus with Oilseed Peptides: An In Silico Approach

Antibiotics ◽  
2021 ◽  
Vol 10 (10) ◽  
pp. 1211
Author(s):  
Joe-Hui Ong ◽  
Wey-Lim Wong ◽  
Fai-Chu Wong ◽  
Tsun-Thai Chai
Keyword(s):  
Complex Formation ◽  
In Silico ◽  
Vibrio Parahaemolyticus ◽  
Water Solubility ◽  
Toxin Binding ◽  
Binding Peptides ◽  
Acute Hepatopancreatic Necrosis Disease ◽  
Hydrolysis Of ◽  
Tryptic Hydrolysis ◽  
Dual Target

Acute hepatopancreatic necrosis disease (AHPND), caused by PirAvp- and PirBvp-releasing Vibrio parahaemolyticus strains, has resulted in massive mortality in shrimp aquaculture. Excessive use of antibiotics for AHPND management has led to antibiotic resistance, highlighting the urgency to search for alternatives. Using an in silico approach, we aimed to discover PirAvp/PirBvp-binding peptides from oilseed meals as alternatives to antibiotics. To search for peptides that remain intact in the shrimp digestive tract, and therefore would be available for toxin binding, we focused on peptides released from tryptic hydrolysis of 37 major proteins from seeds of hemp, pumpkin, rape, sesame, and sunflower. This yielded 809 peptides. Further screening led to 24 peptides predicted as being non-toxic to shrimp, fish, and humans, with thermal stability and low water solubility. Molecular docking on the 24 peptides revealed six dual-target peptides capable of binding to key regions responsible for complex formation on both PirAvp and PirBvp. The peptides (ISYVVQGMGISGR, LTFVVHGHALMGK, QSLGVPPQLGNACNLDNLDVLQPTETIK, ISTINSQTLPILSQLR, PQFLVGASSILR, and VQVVNHMGQK) are 1139–2977 Da in mass and 10–28 residues in length. Such peptides are potential candidates for the future development of peptide-based anti-AHPND agents which potentially mitigate V. parahaemolyticus pathogenesis by intercepting PirAvp/PirBvp complex formation.

Insights on peptide topology in the computational design of protein ligands: the example of lysozyme binding peptides

2021 ◽  
Author(s):  
Cristina Cantarutti ◽  
M. Cristina Vargas ◽  
Cedrix J. Dongmo Foumthuim ◽  
Mireille Dumoulin ◽  
Sara La Manna ◽  
...  
Keyword(s):  
Binding Sites ◽  
In Silico ◽  
Cyclic Peptides ◽  
Computational Design ◽  
Protein Ligands ◽  
Binding Peptides

We compared the ability of in silico generated linear and cyclic peptides to target different binding sites on lysozyme. Results demonstrated that cyclic peptides are optimal for solvent exposed sites, while both topologies can target its pocket.

Abnormal levels of neuropeptide Y and peptide YY in colon in the irritable bowel syndrome (IBS)

2001 ◽  
Vol 120 (5) ◽  
pp. A753-A754
Author(s):  
M SIMREN ◽  
G RINGSTROM ◽  
P STOTZER ◽  
H ABRAHAMSSON ◽  
E BJOMSSON
Keyword(s):  
Irritable Bowel Syndrome ◽  
Neuropeptide Y ◽  
Peptide Yy ◽  
Irritable Bowel

Low cerebrospinal fluid neuropeptide Y concentrations in posttraumatic stress disorder

2009 ◽  
Author(s):  
R. Sah ◽  
N. N. Ekhator ◽  
J. R. Strawn ◽  
F. R. Sallee ◽  
D. G. Baker ◽  
...  
Keyword(s):  
Posttraumatic Stress Disorder ◽  
Cerebrospinal Fluid ◽  
Neuropeptide Y ◽  
Posttraumatic Stress ◽  
Stress Disorder

A systematic review with in silico analysis on transcriptomic profile of gallbladder carcinoma

2020 ◽  
Vol 47 (6) ◽  
pp. 398-408
Author(s):  
Sonam Tulsyan ◽  
Showket Hussain ◽  
Balraj Mittal ◽  
Sundeep Singh Saluja ◽  
Pranay Tanwar ◽  
...  
Keyword(s):  
Systematic Review ◽  
Gallbladder Carcinoma ◽  
In Silico ◽  
In Silico Analysis ◽  
Transcriptomic Profile ◽  
Silico Analysis

Common and well-documented Allele List: unverzichtbares Hilfsmittel für die moderne HLA-Typisierung

2019 ◽  
Vol 9 (04) ◽  
pp. 241-245
Author(s):  
Nils Lachmann ◽  
Diana Stauch ◽  
Axel Pruß
Keyword(s):  
In Silico ◽  
Regionale Unterschiede

ZusammenfassungDie Typisierung der humanen Leukozytenantigene (HLA) vor Organ- und hämatopoetischer Stammzelltransplantation zur Beurteilung der Kompatibilität von Spender und Empfänger wird heutzutage in der Regel molekulargenetisch mittels Amplifikation, Hybridisierung oder Sequenzierung durchgeführt. Durch die exponentiell steigende Anzahl an neu entdeckten HLA-Allelen treten vermehrt Mehrdeutigkeiten, sogenannte Ambiguitäten, in der HLA-Typisierung auf, die aufgelöst werden müssen, um zu einem eindeutigen Ergebnis zu gelangen. Mithilfe kategorisierter Allelfrequenzen (häufig, gut dokumentiert und selten) in Form von CWD-Allellisten (CWD: common and well-documented) ist die In-silico-Auflösung von Ambiguitäten durch den Ausschluss seltener Allele als mögliches Ergebnis realisierbar. Ausgehend von einer amerikanischen CWD-Liste existieren derzeit auch eine europäische, deutsche und chinesische CWD-Liste, die jeweils regionale Unterschiede in den Allelfrequenzen erkennbar werden lassen. Durch die Anwendung von CWD-Allelfiltern in der klinischen HLA-Typisierung können Zeit, Kosten und Arbeitskraft eingespart werden.

Sign in / Sign up

Export Citation Format

Share Document