scholarly journals Uncovering the Early Stages of Domain Melting in Calmodulin with Ultrafast Temperature-Jump Infrared Spectroscopy

2019 ◽  
Vol 123 (41) ◽  
pp. 8733-8739 ◽  
Author(s):  
Lucy Minnes ◽  
Gregory M. Greetham ◽  
Daniel J. Shaw ◽  
Ian P. Clark ◽  
Robby Fritzsch ◽  
...  
Keyword(s):  
Infrared Spectroscopy ◽  
Temperature Jump ◽  
Early Stages

scholarly journals On the Role of Poly-Glutamic Acid in the Early Stages of Iron(III) (Oxy)(hydr)oxide Formation

Minerals ◽  
2021 ◽  
Vol 11 (7) ◽  
pp. 715
Author(s):  
Miodrag J. Lukić ◽  
Felix Lücke ◽  
Teodora Ilić ◽  
Katharina Petrović ◽  
Denis Gebauer
Keyword(s):  
Fourier Transform ◽  
Infrared Spectroscopy ◽  
Phase Separation ◽  
Fourier Transform Infrared Spectroscopy ◽  
Fourier Transform Infrared ◽  
Nucleation Theory ◽  
Classical Nucleation Theory ◽  
Oxide Formation ◽  
Early Stages ◽  
Titration Assay

Nucleation of minerals in the presence of additives is critical for achieving control over the formation of solids in biomineralization processes or during syntheses of advanced hybrid materials. Herein, we investigated the early stages of Fe(III) (oxy)(hydr)oxide formation with/without polyglutamic acid (pGlu) at low driving force for phase separation (pH 2.0 to 3.0). We employed an advanced pH-constant titration assay, X-ray diffraction, thermal analysis with mass spectrometry, Fourier Transform infrared spectroscopy, and scanning electron microscopy. Three stages were observed: initial binding, stabilization of Fe(III) pre-nucleation clusters (PNCs), and phase separation, yielding Fe(III) (oxy)(hydr)oxide. The data suggest that organic–inorganic interactions occurred via binding of olation Fe(III) PNC species. Fourier Transform Infrared Spectroscopy (FTIR) analyses revealed a plausible interaction motif and a conformational adaptation of the polypeptide. The stabilization of the aqueous Fe(III) system against nucleation by pGlu contrasts with the previously reported influence of poly-aspartic acid (pAsp). While this is difficult to explain based on classical nucleation theory, alternative notions such as the so-called PNC pathway provide a possible rationale. Developing a nucleation theory that successfully explains and predicts distinct influences for chemically similar additives like pAsp and pGlu is the Holy Grail toward advancing the knowledge of nucleation, early growth, and structure formation.

Early Stages during the Oxidation of HCOOH on Single-Crystal Pt Electrodes As Characterized by Infrared Spectroscopy

Langmuir ◽  
1996 ◽  
Vol 12 (17) ◽  
pp. 4260-4265 ◽  
Author(s):  
Teresa Iwasita ◽  
Xinghua Xia ◽  
Enrique Herrero ◽  
Hans-Dieter Liess
Keyword(s):  
Infrared Spectroscopy ◽  
Single Crystal ◽  
Early Stages ◽  
Pt Electrodes

scholarly journals Direct Evidence of Catalytic Heterogeneity in Lactate Dehydrogenase by Temperature Jump Infrared Spectroscopy

2014 ◽  
Vol 118 (37) ◽  
pp. 10854-10862 ◽  
Author(s):  
Michael J. Reddish ◽  
Huo-Lei Peng ◽  
Hua Deng ◽  
Kunal S. Panwar ◽  
Robert Callender ◽  
...  
Keyword(s):  
Infrared Spectroscopy ◽  
Lactate Dehydrogenase ◽  
Direct Evidence ◽  
Temperature Jump ◽  
Catalytic Heterogeneity

Fast Events in Protein Folding Initiated by Laser-Induced Temperature Jump and Probed by Time-Resolved Infrared Spectroscopy

1995 ◽  
pp. 105-106
Author(s):  
Skip Williams ◽  
Timothy P. Causgrove ◽  
Rudolf Gilmanshin ◽  
R. Brian Dyer ◽  
William H. Woodruff ◽  
...  
Keyword(s):  
Protein Folding ◽  
Infrared Spectroscopy ◽  
Temperature Jump ◽  
Time Resolved ◽  
Time Resolved Infrared Spectroscopy
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