Molecular Mechanism of the Cell Membrane Pore Formation Induced by Bubble Stable Cavitation

2018 ◽  
Vol 123 (1) ◽  
pp. 71-78 ◽  
Author(s):  
Viet Hoang Man ◽  
Phan Minh Truong ◽  
Mai Suan Li ◽  
Junmei Wang ◽  
Nguyen-Thi Van-Oanh ◽  
...  
Keyword(s):  
Cell Membrane ◽  
Molecular Mechanism ◽  
Pore Formation ◽  
Membrane Pore

Membrane pore formation and ion selectivity of the Ebola virus delta peptide

2019 ◽  
Vol 21 (10) ◽  
pp. 5578-5585 ◽  
Author(s):  
Rudramani Pokhrel ◽  
Elumalai Pavadai ◽  
Bernard S. Gerstman ◽  
Prem P. Chapagain
Keyword(s):  
Cell Membrane ◽  
Host Cell ◽  
Pore Formation ◽  
Ebola Virus ◽  
Ion Selectivity ◽  
Membrane Properties ◽  
Host Cell Membrane ◽  
Membrane Pore

The Ebola virus delta peptide homo-oligomerizes in the host cell membrane to form amphipathic pores that alter the membrane properties.

scholarly journals Molecular mechanism of pyroptosis of mononuclear macrophages induced by pathogenic E. coli high pathogenicity island (HPI) in Yunnan Saba pigs

2020 ◽  
Author(s):  
Chunlan Shan ◽  
Shushu Miao ◽  
Chaoying Liu ◽  
Weiwei Zhao ◽  
Bo Zhang ◽  
...  
Keyword(s):  
Cell Membrane ◽  
Molecular Mechanism ◽  
Pathogenicity Island ◽  
Control Group ◽  
Tunel Staining ◽  
Membrane Pore ◽  
Expression Levels ◽  
E Coli ◽  
Caspase 1 ◽  
High Pathogenicity

Abstract Background In this study we evaluated the molecular mechanism by which pyroptosis is induced in mononuclear macrophages isolated from Saba pigs following infection with pathogenic E. coli high pathogenicity island (HPI). Mononuclear macrophages were divided into four treatment groups: control, Lipopolysaccharide (LPS) + adenosine triphosphate (ATP), HPI positive (+) strain and HPI negative (-) strain. The mononuclear macrophages and their culture supernatants were collected at 0.5, 3, 6, 9, 12 and 24 h after infection. DNA changes were detected by TUNEL staining and the integrity of the cell membrane was evaluated by propidium iodide (PI) staining. Changes in mRNA expression levels of NLRP3, caspase-1, IL-1β, and IL-18 gene in mononuclear macrophages were analyzed by quantitative real-time polymerase chain reaction (RT-PCR) and caspase-1 protein expression was detected by indirect immunofluorescence. IL-1β and IL-18 concentration in the mononuclear macrophage culture supernatant were measured by ELISA. Results Compared with the control group, TUNEL and PI staining of mononuclear macrophages was significantly increased following infection with the HPI + /HPI - strains ( P < 0.01 or P < 0.05), with significantly higher levels detected in the HPI + group compared with those in the HPI - group ( P < 0.01 and P < 0.05). Compared with the control group, the expression levels of NLRP3, caspase-1, IL-1β, and IL-18 in the HPI groups were upregulated after pathogenic E. coli infection, with significantly higher levels detected in the HPI + group compared with those in the HPI - group ( P < 0.01 or P < 0.05). Conclusions These findings showed that pathogenic E. coli HPI infection of Saba pigs results induced pyroptosis of mononuclear macrophages characterized by increased expression of NLRP3, caspase-1, IL-1β and IL-18 mRNA in mononuclear macrophages, the induction of cell membrane pore formation, nuclear DNA damage, and the secretion of IL-1β and IL-18 to enhance the inflammatory response.

scholarly journals Induction of macrophage pyroptosis-related factors by pathogenic E. coli high pathogenicity island (HPI) in Yunnan Saba pigs

2021 ◽  
Vol 17 (1) ◽  
Author(s):  
Chunlan Shan ◽  
Shushu Miao ◽  
Chaoying Liu ◽  
Bo Zhang ◽  
Weiwei Zhao ◽  
...  
Keyword(s):  
Cell Membrane ◽  
Inflammatory Diseases ◽  
Pathogenicity Island ◽  
Cell Membrane Permeability ◽  
Inflammatory Factor ◽  
Related Factors ◽  
Membrane Pore ◽  
E Coli ◽  
Caspase 1 ◽  
High Pathogenicity

Abstract Background Pyroptosis plays a pivotal role in the pathogenesis of many inflammatory diseases. The molecular mechanism by which pyroptosis is induced in macrophages following infection with pathogenic E. coli high pathogenicity island (HPI) will be evaluated in our study. Results After infection with the HPI+/HPI− strains and LPS, decreased macrophage cell membrane permeability and integrity were demonstrated with propidium iodide (PI) staining and the lactate dehydrogenase (LDH) assay. HPI+/HPI−-infection was accompanied by upregulated expression levels of NLRP3, ASC, caspase-1, IL-1β, IL-18 and GSDMD, with significantly higher levels detected in the HPI+ group compared to those in the HPI− group (P < 0.01 or P < 0.05). HPI+ strain is more pathogenic than HPI− strain. Conclusion Our findings indicate that pathogenic E. coli HPI infection of Saba pigs causes pyroptosis of macrophages characterized by upregulated expression of pyroptosis key factors in the NLRP3/ASC/caspase-1 signaling pathway, direct cell membrane pore formation, and secretion of the inflammatory factor IL-1β and IL-18 downstream of NLRP3 and caspase-1 activation to enhance the inflammatory response.

Membrane pore formation at protein–lipid interfaces

2014 ◽  
Vol 39 (11) ◽  
pp. 510-516 ◽  
Author(s):  
Robert J.C. Gilbert ◽  
Mauro Dalla Serra ◽  
Christopher J. Froelich ◽  
Mark I. Wallace ◽  
Gregor Anderluh
Keyword(s):  
Pore Formation ◽  
Membrane Pore

scholarly journals Molecular Basis for Membrane Pore Formation by Bax Protein Carboxyl Terminus

Biochemistry ◽  
2012 ◽  
Vol 51 (46) ◽  
pp. 9406-9419 ◽  
Author(s):  
Suren A. Tatulian ◽  
Pranav Garg ◽  
Kathleen N. Nemec ◽  
Bo Chen ◽  
Annette R. Khaled
Keyword(s):  
Molecular Basis ◽  
Pore Formation ◽  
Carboxyl Terminus ◽  
Membrane Pore ◽  
Bax Protein

scholarly journals Molecular Mechanism of Polycation-Induced Pore Formation in Biomembranes

2018 ◽  
Vol 5 (2) ◽  
pp. 780-794 ◽  
Author(s):  
Neha Awasthi ◽  
Wojciech Kopec ◽  
Natalia Wilkosz ◽  
Dorota Jamróz ◽  
Jochen S. Hub ◽  
...  
Keyword(s):  
Molecular Mechanism ◽  
Pore Formation
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